Mouse Model of In Situ Thromboembolic Stroke and Reperfusion

2016 ◽  
pp. 59-68
Author(s):  
Orset Cyrille ◽  
Le Béhot Audrey ◽  
Bonnet Anne-Laure ◽  
Maysami Samaneh ◽  
Vivien Denis
Keyword(s):  
Mouse Model ◽  
Thromboembolic Stroke

scholarly journals A Mouse Model of Hemorrhagic Transformation by Delayed Tissue Plasminogen Activator Administration After In Situ Thromboembolic Stroke

Stroke ◽  
2011 ◽  
Vol 42 (1) ◽  
pp. 196-203 ◽  
Author(s):  
Isaac García-Yébenes ◽  
Mónica Sobrado ◽  
Juan G. Zarruk ◽  
Mar Castellanos ◽  
Natalia Pérez de la Ossa ◽  
...  
Keyword(s):  
Mouse Model ◽  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Hemorrhagic Transformation ◽  
Thromboembolic Stroke ◽  
Tissue Plasminogen

scholarly journals Mouse Model of In Situ Thromboembolic Stroke and Reperfusion

Stroke ◽  
2007 ◽  
Vol 38 (10) ◽  
pp. 2771-2778 ◽  
Author(s):  
Cyrille Orset ◽  
Richard Macrez ◽  
Alan R. Young ◽  
Didier Panthou ◽  
Eduardo Angles-Cano ◽  
...  
Keyword(s):  
Mouse Model ◽  
Thromboembolic Stroke

scholarly journals TMOD-24. GENERATION AND CHARACTERIZATION OF A NOVEL TRANSGENIC IDO REPORTER MOUSE FOR IDO POSTTRANSLATIONAL MODIFICATION ANALYSIS IN SITU

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii233-ii233
Author(s):  
April Bell ◽  
Lijie Zhai ◽  
Erik Ladomersky ◽  
Kristen Lauing ◽  
Lakshmi Bollu ◽  
...  
Keyword(s):  
Mouse Model ◽  
Tumor Cell ◽  
Central Nervous System Tumor ◽  
Post Translational Modifications ◽  
Reporter Mouse ◽  
C Terminus ◽  
Human Gbm

Abstract Glioblastoma (GBM) is the most common and aggressive primary central nervous system tumor in adults with a median survival of 14.6 months. GBM is a potently immunosuppressive cancer due in-part to the prolific expression of immunosuppressive indoleamine 2,3 dioxygenase 1 (IDO). Tumor cell IDO facilitates the intratumoral accumulation of regulatory T cells (Tregs; CD4+CD25+FoxP3+). Although immunosuppressive IDO activity is canonically characterized by the conversion of tryptophan into kynurenine, we have utilized transgenic and syngeneic mouse models and mutant glioma lines to demonstrate that tumor cell IDO increases Treg accumulation independent of tryptophan metabolism. Here, we address the gap in our understanding of IDO signaling activity in vivo. Subcutaneously-engrafted human GBM expressing human IDO-GFP cDNA was isolated from immunodeficient humanized NSG-SGM3 mice. The tumor was immunoprecipitated for the GFP tag using GFP-TRAP followed by mass spectrometry which revealed a novel methylation site on a lysine residue at amino acid 373 in the IDO C-terminus region. Western blot analysis of IDO protein also revealed the presence of tyrosine phosphorylation. Additionally, we recently created a new transgenic IDO reporter mouse model whereby endogenous IDO is fused to GFP via a T2A linker (IDO→GFP). This model allows for the isolation of IDO+ cells in real-time and without causing cell death, thereby creating the opportunity for downstream molecular analysis of in situ-isolated GFP+ cells. Collectively, our work suggests that IDO non-enzyme activity may involve the post-translational modifications we recently identified. As IDO activity may differ between in vitro and in vivo modeling systems, we will use the new IDO→GFP reporter mouse model for an improved mechanistic understanding of how immunosuppressive IDO facilitates Treg accumulation in vivo.

scholarly journals Glucose Uptake and Intracellular pH in a Mouse Model of Ductal Carcinoma In situ (DCIS) Suggests Metabolic Heterogeneity

2016 ◽  
Vol 4 ◽  
Author(s):  
Rebecca C. Lobo ◽  
Neil E. Hubbard ◽  
Patrizia Damonte ◽  
Hidetoshi Mori ◽  
Zsófia Pénzváltó ◽  
...  
Keyword(s):  
Mouse Model ◽  
Glucose Uptake ◽  
Intracellular Ph ◽  
Ductal Carcinoma In Situ ◽  
Carcinoma In Situ ◽  
Ductal Carcinoma ◽  
Metabolic Heterogeneity

scholarly journals Dynamic FDG-PET Imaging to Differentiate Malignancies from Inflammation in Subcutaneous and In Situ Mouse Model for Non-Small Cell Lung Carcinoma (NSCLC)

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0139089 ◽  
Author(s):  
Zhen Yang ◽  
Yunlong Zan ◽  
Xiujuan Zheng ◽  
Wangxi Hai ◽  
Kewei Chen ◽  
...  
Keyword(s):  
Mouse Model ◽  
Lung Carcinoma ◽  
Pet Imaging ◽  
Fdg Pet ◽  
Small Cell Lung Carcinoma ◽  
Small Cell ◽  
Small Cell Lung ◽  
Cell Lung Carcinoma

Chemical shift sodium imaging in a mouse model of thromboembolic stroke at 9.4 T

2011 ◽  
Vol 34 (4) ◽  
pp. 935-940 ◽  
Author(s):  
Patrick M. Heiler ◽  
Friederike L. Langhauser ◽  
Friedrich Wetterling ◽  
Saema Ansar ◽  
Saskia Grudzenski ◽  
...  
Keyword(s):  
Chemical Shift ◽  
Mouse Model ◽  
Thromboembolic Stroke ◽  
Sodium Imaging

scholarly journals A novel mouse model of thromboembolic stroke

2015 ◽  
Vol 256 ◽  
pp. 203-211 ◽  
Author(s):  
Yingxin Chen ◽  
Wenbin Zhu ◽  
Wenri Zhang ◽  
Nicole Libal ◽  
Stephanie J. Murphy ◽  
...  
Keyword(s):  
Mouse Model ◽  
Thromboembolic Stroke

In situ bioluminescent imaging of xenograft progression in an orthotopic mouse model of HNSCC

2008 ◽  
Vol 26 (15_suppl) ◽  
pp. 17006-17006
Author(s):  
R. A. Madero-Visbal ◽  
I. C. Hernandez ◽  
J. N. Myers ◽  
C. H. Baker ◽  
T. D. Shellenberger
Keyword(s):  
Mouse Model ◽  
Bioluminescent Imaging ◽  
Orthotopic Mouse Model

scholarly journals In Situ Transplantation of Alginate Bioencapsulated Adipose Tissues Derived Stem Cells (ADSCs) via Hepatic Injection in a Mouse Model

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0138184 ◽  
Author(s):  
Mong-Jen Chen ◽  
Yuanqing Lu ◽  
Nicholas E. Simpson ◽  
Mark J. Beveridge ◽  
Ahmed S. Elshikha ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mouse Model ◽  
Adipose Tissues
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