Methods to Establish Drosophila Cell Lines

2016 ◽  
pp. 333-351 ◽  
Author(s):  
Alain Debec ◽  
Timothy L. Megraw ◽  
Antoine Guichet
Keyword(s):  
Cell Lines ◽  
Drosophila Cell

DIFFERENTIAL EFFECTS OF 20-HYDROXYECDYSONE ON CELL INTERACTIONS AND SURFACE PROTEINS IN DROSOPHILA CELL LINES

Ecdysone ◽  
1986 ◽  
pp. 211-224
Author(s):  
WAYNE L. RICKOLL ◽  
JANICE A. STACHOWIAK ◽  
SAMUEL GALEWSKY ◽  
MICHAEL A. JUNIO ◽  
ELIZABETH S. HAYES
Keyword(s):  
Cell Lines ◽  
Cell Interactions ◽  
Surface Proteins ◽  
Drosophila Cell ◽  
Differential Effects

Reoviruses of Drosophila and Ceratitis populations and of Drosophila cell lines: A possible new genus of the Reoviridae family

1981 ◽  
Vol 132 (2) ◽  
pp. 261-270 ◽  
Author(s):  
N. Plus ◽  
L. Gissman ◽  
J.C. Veyrunes ◽  
H. Pfister ◽  
E. Gateff
Keyword(s):  
Cell Lines ◽  
New Genus ◽  
Drosophila Cell

scholarly journals Establishment and characterization of Drosophila cell lines mutant for heparan sulfate modifying enzymes

Glycobiology ◽  
2019 ◽  
Vol 29 (6) ◽  
pp. 479-489
Author(s):  
Eriko Nakato ◽  
Xin Liu ◽  
Inger Eriksson ◽  
Maki Yamamoto ◽  
Akiko Kinoshita-Toyoda ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Cell Lines ◽  
Drosophila Cell

scholarly journals Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9

Biology Open ◽  
2013 ◽  
Vol 3 (1) ◽  
pp. 42-49 ◽  
Author(s):  
A. R. Bassett ◽  
C. Tibbit ◽  
C. P. Ponting ◽  
J.-L. Liu
Keyword(s):  
Homologous Recombination ◽  
Cell Lines ◽  
Drosophila Cell

A single point mutation in Drosophila dihydrofolate reductase confers methotrexate resistance to a transgenic CHO cell line

Genome ◽  
2003 ◽  
Vol 46 (4) ◽  
pp. 707-715 ◽  
Author(s):  
K Neumann ◽  
K M Al-Batayneh ◽  
M J Kuiper ◽  
J Parsons-Sheldrake ◽  
M G Tyshenko ◽  
...  
Keyword(s):  
Cell Line ◽  
Point Mutation ◽  
Cell Lines ◽  
Dihydrofolate Reductase ◽  
Cho Cells ◽  
Chinese Hamster ◽  
Single Point Mutation ◽  
Parental Line ◽  
Wild Type ◽  
Drosophila Cell

Sequence analysis of a cDNA encoding dihydrofolate reductase (DHFR) from a selected methotrexate-resistant Drosophila melanogaster cell line (S3MTX) revealed a substitution of Gln for Leu at position 30. Although the S3MTX cells were ~1000 fold more resistant to methotrexate (MTX), the karyotype was similar to the parental line and did not show elongated chromosomes. Furthermore, kinetic analysis of the recombinant enzyme showed a decreased affinity for MTX by the mutant DHFR. To determine if the resistance phenotype could be attributed to the mutant allele, Drosophila Dhfr cDNAs isolated from wild type and S3MTX cells were expressed in Chinese hamster ovary (CHO) cells lacking endogenous DHFR. The heterologous insect DHFRs were functional in transgenic clonal cell lines, showing ~400-fold greater MTX resistance in the cell line transfected with the mutant Dhfr than the wild type Dhfr. Resistance to other antifolates in the CHO cells was consistent with the drug sensitivities seen in the respective Drosophila cell lines. ELevated Levels of Dhfr transcript and DHFR in transgenic CHO cells bearing the mutant cDNA were not seen. Taken together, these results demonstrate that a single substitution in Drosophila DHFR alone can confer Levels of MTX resistance comparable with that observed after considerable gene amplification in mammalian cells.Key words: dihydrofolate reductase, methotrexate, drug resistance, point mutation.

P1-139: Drosophila cell lines as a model for hypothesis-driven RNAi-based screens for amyloid action

2008 ◽  
Vol 4 ◽  
pp. T247-T247
Author(s):  
Steven D. Buckingham ◽  
David B. Sattelle
Keyword(s):  
Cell Lines ◽  
Drosophila Cell
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