Probing Collagen Organization: Practical Guide for Second-Harmonic Generation (SHG) Imaging

2017 ◽  
pp. 409-425 ◽  
Author(s):  
Riccardo Cicchi ◽  
Francesco S. Pavone
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Collagen Organization ◽  
Practical Guide

scholarly journals Second harmonic generation microscopy of collagen organization in tunable, environmentally responsive alginate hydrogels

2019 ◽  
Vol 7 (3) ◽  
pp. 1188-1199 ◽  
Author(s):  
Anuraag Boddupalli ◽  
Kaitlin M. Bratlie
Keyword(s):  
Tissue Engineering ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Engineering Applications ◽  
Second Harmonic Generation Microscopy ◽  
Collagen Organization ◽  
Environmentally Responsive ◽  
Alginate Hydrogels

We fabricated photocrosslinked, environmentally responsive alginate hydrogels for tissue engineering applications.

scholarly journals Collagen Organization Within the Cartilage of Trpv4 −/− Mice Studied with Two‐Photon Microscopy and Polarized Second Harmonic Generation

2019 ◽  
Vol 97 (5) ◽  
pp. 504-514
Author(s):  
M. Rocio Servin‐Vences ◽  
Kate Poole ◽  
Anje Sporbert ◽  
Gary R. Lewin ◽  
Anca Margineanu
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Collagen Organization

scholarly journals Second-harmonic generation microscopy analysis reveals proteoglycan decorin is necessary for proper collagen organization in prostate

2019 ◽  
Vol 24 (06) ◽  
pp. 1 ◽  
Author(s):  
Kirby R. Campbell ◽  
Rajeev Chaudhary ◽  
Monica Montano ◽  
Renato V. Iozzo ◽  
Wade A. Bushman ◽  
...  
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Second Harmonic Generation Microscopy ◽  
Collagen Organization ◽  
Microscopy Analysis

scholarly journals Collagen reorganization in cartilage under strain probed by polarization sensitive second harmonic generation microscopy

2019 ◽  
Vol 16 (150) ◽  
pp. 20180611 ◽  
Author(s):  
Jessica C. Mansfield ◽  
Vipul Mandalia ◽  
Andrew Toms ◽  
C. Peter Winlove ◽  
Sophie Brasselet
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Collagen Fibril ◽  
Second Harmonic ◽  
Light And Electron Microscopy ◽  
Tensile Modulus ◽  
Diffraction Limit ◽  
Superficial Zone ◽  
Osteoarthritic Cartilage ◽  
Collagen Organization

Type II collagen fibril diameters in cartilage are beneath the diffraction limit of optical microscopy, which makes the assessment of collagen organization very challenging. In this work we use polarization sensitive second harmonic generation (P-SHG) imaging to map collagen organization in articular cartilage, addressing in particular its behaviour under strain and changes which occur in osteoarthritis. P-SHG yields two parameters, molecular order and orientation, which provide measures of the degree of organization both at the molecular scale (below the diffraction limit) and above a few hundred nanometres (at the image pixel size). P-SHG clearly demonstrates the zonal collagen architecture and reveals differences in the structure of the fibrils around chondrocytes. P-SHG also reveals sub-micron scale fibril re-organization in cartilage strips exposed to tensile loading, with an increase in local organization in the superficial zone which weakly correlates with tensile modulus. Finally, P-SHG is used to investigate osteoarthritic cartilage from total knee replacement surgery, and reveals widespread heterogeneity across samples both microscale fibril orientations and their sub-micron organization. By addressing collagen fibril structure on scales intermediate between conventional light and electron microscopy, this study provides new insights into collagen micromechanics and mechanisms of degradation.

Test of directional photoionisation models of second harmonic generation in optical fibres

1992 ◽  
Vol 139 (2) ◽  
pp. 133 ◽  
Author(s):  
N.M. Lawandy ◽  
T.J. Driscoll ◽  
C.L. Adler ◽  
N.M. Lawandy
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Optical Fibres
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