In Vitro Conservation of Date Palm Shoot-Tip Explants and Callus Cultures Under Minimal Growth Conditions

2017 ◽  
pp. 49-59
Author(s):  
Maiada M. El-Dawayati
Keyword(s):  
Date Palm ◽  
Growth Conditions ◽  
Callus Cultures ◽  
Shoot Tip ◽  
In Vitro Conservation ◽  
Minimal Growth ◽  
Shoot Tip Explants

scholarly journals IN VITRO CONSERVATION OF DATE PALM SHOOT TIP EXPLANTS UNDER MINIMAL GROWTH CONDITION

2013 ◽  
Vol 91 (3) ◽  
pp. 1043-1062
Author(s):  
MAIADA M. EL-DAWAYATI ◽  
ELSAYED I. BAKER ◽  
AMINA H. GOMAA ◽  
ZEINAB E. ZAYED
Keyword(s):  
Growth Condition ◽  
Date Palm ◽  
Shoot Tip ◽  
In Vitro Conservation ◽  
Minimal Growth ◽  
Shoot Tip Explants

scholarly journals In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions

2019 ◽  
Vol 43 ◽  
Author(s):  
Tecla dos Santos Silva ◽  
Cristina Ferreira Nepomuceno ◽  
Taliane Leila Soares ◽  
José Raniere Ferreira de Santana
Keyword(s):  
Aerial Part ◽  
Dry Mass ◽  
Growth Conditions ◽  
In Vitro Conservation ◽  
Ex Situ ◽  
Minimal Growth ◽  
Survival Percentage ◽  
Medicinal Substances ◽  
Osmotic Agents

ABSTRACT Poincianella pyramidalis (Tul.) L.P. Queiroz, is an arboreal species endemic to Brazil’s Caatinga biome and an important source of lumber and also of medicinal substances. This study aimed to evaluate the effect of the osmotic agents sucrose, sorbitol and mannitol and the growth retardant paclobutrazol (PBZ) on the induction of slow growth in vitro of P. pyramidalis, seeking to establish alternative techniques for ex situ conservation of the species. In the first experiment, plantlets with seven days of age were inoculated on Woody Plant Medium (WPM) supplemented with four concentrations of sucrose (87.64, 131.46, 175.28 or 219.10 mM) combined with 0.0 or 87.64 mM sorbitol or mannitol. In the second experiment, these plantlets were inoculated on WPM medium with 0.0, 1.5, 3.0, 4.5 or 6.0 µM PBZ and supplemented with 87.64 mM sucrose. For both experiments, the survival percentage of the plants was evaluated every 60 days, and at the end of 240 days, the following parameters were recorded: number of green leaves and senescent leaves; length of the aerial part and longest root; and dry mass of the aerial part and roots. The best results to maintain the quality of the P. pyramidalis plants conserved in vitro were obtained on medium supplemented with 219.10 mM sucrose for up to 240 days without subculture. With respect to PBZ, the concentration of 6.0 μM can be recommended for in vitro conservation of P. pyramidalis for 240 days without the need of subculturing.

scholarly journals In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagation

2017 ◽  
Vol 52 (12) ◽  
pp. 1286-1290 ◽  
Author(s):  
Hugo Teixeira Gomes ◽  
Patrícia Monah Cunha Bartos ◽  
Maíra Teixeira de Andrade ◽  
Raphael Ferreira Almeida ◽  
Luciana Florencio de Lacerda ◽  
...  
Keyword(s):  
Large Scale ◽  
Growth Conditions ◽  
In Vitro Conservation ◽  
Nodal Segments ◽  
Minimal Growth

Abstract: The objective of this work was to evaluate the micropropagation of blackberry (Rubus spp.) cultivars, after in vitro conservation under minimal growth conditions. Nodal segments of the 'Guarani', 'Caingangue', 'Ébano', and 'Xavante' genotypes were conserved under minimal growth conditions at 20ºC, for 15 months. Microshoots were regenerated and multiplied by up to five successive subcultures, when they were rooted and acclimatized. After 30 days of acclimatization in a greenhouse, rooted plantlets showed no significant losses. Blackberry cultivars can be conserved in vitro for 15 months, without subcultures and, after this time, they can be micropropagated on a large-scale, maintaining the regenerative potential and multiplication.

scholarly journals In vitro propagation and cytological analysis of Sophora mollis Royle: an endangered medicinal shrub

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Aakriti Bhandari ◽  
Harminder Singh ◽  
Amber Srivastava ◽  
Puneet Kumar ◽  
G. S. Panwar ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Average Length ◽  
Germplasm Conservation ◽  
Basic Chromosome Number ◽  
Shoot Tip ◽  
Ex Situ ◽  
Ms Medium ◽  
Basic Chromosome ◽  
Shoot Tip Explants

Abstract Background Sophora mollis Royle (family Fabaceae, subfamily-Papilionaceae) is a multipurpose legume distributed in plains and foothills of the North-West Himalaya to Nepal and is facing high risk of extinction due to habitat loss and exploitation by the local people for its fuel and fodder values. Therefore, the present study was conducted to standardize a micropropagation protocol for Sophora mollis by using shoot tip explants and to study the meiotic chromosome count in the species. Results Multiple shoots were induced in shoot tip explants of Sophora mollis in Murashige and Skoog medium supplemented with different concentrations of cytokinins alone (BAP, TDZ, and Kinetin) and in combination with varying concentrations of NAA. MS medium supplemented with BAP (8.9 μM) was observed to be the optimal medium for multiple shoot induction and maximum 25.32 shoots per explant was obtained with average length of 4.5 ± 0.8 cm. In vitro developed shoots were transferred onto rooting media supplemented with different concentrations of auxin (IAA, IBA, and NAA). Maximum 86% rooting was observed in half-strength MS medium supplemented with 21.20 μM NAA with an average of 21.26 roots per culture. In vitro raised plantlets were adapted to greenhouse for better acclimatization and 60% plants were successfully transferred to the open environment. Based on the chromosome counts available from the literature and the current study, the species tend to show a basic chromosome number of x = 9. Conclusion The micropropagation protocol standardized can be helpful for the ex situ mass multiplication and germplasm conservation of the endangered species. Moreover, the ex situ conservation approach will be helpful in actively bridging the gap between ex situ and in situ approaches through the reintroduction of species in the wild. The cytological studies revealed the basic chromosome number x = 9 of the species.

scholarly journals In vitro Propagation of Solanum capsicoides All. – An Important Therapeutic Agent 'Kantakari'

1970 ◽  
Vol 20 (2) ◽  
pp. 179-184
Author(s):  
N.P. Anish ◽  
M.G. Rajesh ◽  
Jiby Elias ◽  
N. Jayan
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Therapeutic Agent ◽  
Shoot Tip ◽  
Shoot Induction ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants from in vitro germinated seedlings of Solanum capsicoides All. inoculated on MS containing 2 mg/l BA produced maximum shoot induction response (26 shoots per explant). Rooting of the microshoots (19.4 roots per explant) was obtained better in half strength of MS supplemented with NAA (0.5 mg/l). Well rooted plantlets were successfully hardened with 80 per cent survival rate.   Key words: Solanum capsicoides, Propagation, Therapeutic agent   D.O.I. 10.3329/ptcb.v20i2.6912   Plant Tissue Cult. & Biotech. 20(2): 179-184, 2010 (December)

In vitro storage ofXanthosoma spp. under minimal growth conditions

1994 ◽  
Vol 36 (3) ◽  
pp. 309-316 ◽  
Author(s):  
E. A. Zandvoort ◽  
M. J. H. Hulshof ◽  
G. Staritsky
Keyword(s):  
Growth Conditions ◽  
Minimal Growth ◽  
In Vitro Storage
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