In vitro storage ofXanthosoma spp. under minimal growth conditions

1994 ◽  
Vol 36 (3) ◽  
pp. 309-316 ◽  
Author(s):  
E. A. Zandvoort ◽  
M. J. H. Hulshof ◽  
G. Staritsky
Keyword(s):  
Growth Conditions ◽  
Minimal Growth ◽  
In Vitro Storage

In vitro storage of cedar shoot cultures under minimal growth conditions

2006 ◽  
Vol 25 (7) ◽  
pp. 636-642 ◽  
Author(s):  
Begoña Renau-Morata ◽  
Isabel Arrillaga ◽  
Juan Segura
Keyword(s):  
Growth Conditions ◽  
Shoot Cultures ◽  
Minimal Growth ◽  
In Vitro Storage

scholarly journals In vitro conservation of Vanilla planifolia hybrids in minimal growth conditions

2021 ◽  
Vol 13 (12) ◽  
Author(s):  
Delfino Reyes Lopez ◽  
Enrique Hernández Leal ◽  
Carlos Roman Castillo Martínez ◽  
Carlos Hugo Avendaño Arrazate ◽  
Tarsicio Corona Torres ◽  
...  
Keyword(s):  
Design Methodology ◽  
Culture Medium ◽  
Growth Conditions ◽  
Vanilla Planifolia ◽  
Minimal Growth ◽  
Medium Components ◽  
Differential Behavior ◽  
Different Doses ◽  
Different Origin

Objective: To maintain minimal growth in in vitro Vanilla planifolia hybrids. Design/Methodology/Approach: Explants of seven interspecific hybrids of V. planifolia with different origin parents were used. The treatments consisted of different doses of mannitol and sucrose in the culture medium which varied from 0, 5, 10, 15, 20, 25 and 30 g L-1.The number of nodes, shoots and roots was recorded every 30 d for six months. Results: 30 g L-1 mannitol and no sucrose in the culture medium allowed minimal growth in most of the hybrids. The higher the mannitol and lower the sucrose content, the length, number of between nodes, shoots and roots of the explants was lower (P?0.05). Limitations of the study/implications: There is a differential behavior between the biological material and the used culture medium, particularly in hybrids, due to their new genetic combinations. Therefore, for their conservation, the culture medium components must be adjusted. Conclusions: 30 g L-1 mannitol without sucrose in in vitro culture medium significantly reduces growth during 180 d in vanilla hybrids.

scholarly journals In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions

2019 ◽  
Vol 43 ◽  
Author(s):  
Tecla dos Santos Silva ◽  
Cristina Ferreira Nepomuceno ◽  
Taliane Leila Soares ◽  
José Raniere Ferreira de Santana
Keyword(s):  
Aerial Part ◽  
Dry Mass ◽  
Growth Conditions ◽  
In Vitro Conservation ◽  
Ex Situ ◽  
Minimal Growth ◽  
Survival Percentage ◽  
Medicinal Substances ◽  
Osmotic Agents

ABSTRACT Poincianella pyramidalis (Tul.) L.P. Queiroz, is an arboreal species endemic to Brazil’s Caatinga biome and an important source of lumber and also of medicinal substances. This study aimed to evaluate the effect of the osmotic agents sucrose, sorbitol and mannitol and the growth retardant paclobutrazol (PBZ) on the induction of slow growth in vitro of P. pyramidalis, seeking to establish alternative techniques for ex situ conservation of the species. In the first experiment, plantlets with seven days of age were inoculated on Woody Plant Medium (WPM) supplemented with four concentrations of sucrose (87.64, 131.46, 175.28 or 219.10 mM) combined with 0.0 or 87.64 mM sorbitol or mannitol. In the second experiment, these plantlets were inoculated on WPM medium with 0.0, 1.5, 3.0, 4.5 or 6.0 µM PBZ and supplemented with 87.64 mM sucrose. For both experiments, the survival percentage of the plants was evaluated every 60 days, and at the end of 240 days, the following parameters were recorded: number of green leaves and senescent leaves; length of the aerial part and longest root; and dry mass of the aerial part and roots. The best results to maintain the quality of the P. pyramidalis plants conserved in vitro were obtained on medium supplemented with 219.10 mM sucrose for up to 240 days without subculture. With respect to PBZ, the concentration of 6.0 μM can be recommended for in vitro conservation of P. pyramidalis for 240 days without the need of subculturing.

scholarly journals In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagation

2017 ◽  
Vol 52 (12) ◽  
pp. 1286-1290 ◽  
Author(s):  
Hugo Teixeira Gomes ◽  
Patrícia Monah Cunha Bartos ◽  
Maíra Teixeira de Andrade ◽  
Raphael Ferreira Almeida ◽  
Luciana Florencio de Lacerda ◽  
...  
Keyword(s):  
Large Scale ◽  
Growth Conditions ◽  
In Vitro Conservation ◽  
Nodal Segments ◽  
Minimal Growth

Abstract: The objective of this work was to evaluate the micropropagation of blackberry (Rubus spp.) cultivars, after in vitro conservation under minimal growth conditions. Nodal segments of the 'Guarani', 'Caingangue', 'Ébano', and 'Xavante' genotypes were conserved under minimal growth conditions at 20ºC, for 15 months. Microshoots were regenerated and multiplied by up to five successive subcultures, when they were rooted and acclimatized. After 30 days of acclimatization in a greenhouse, rooted plantlets showed no significant losses. Blackberry cultivars can be conserved in vitro for 15 months, without subcultures and, after this time, they can be micropropagated on a large-scale, maintaining the regenerative potential and multiplication.

Long-term in vitro Storage Technique of Valuable Birch Genotypes and Plant Production on its Basis

2019 ◽  
pp. 57-67
Author(s):  
T.M. Tabatskaya ◽  
N.I. Vnukova
Keyword(s):  
Plant Production ◽  
High Survival ◽  
Ex Vitro ◽  
Regenerative Capacity ◽  
In Vitro Storage ◽  
Interspecific Differences ◽  
Regenerated Plants ◽  
Growing Conditions

A technique for the long-term (up to 27 years) in vitro storage of valuable birch genotypes under normal (25 °C, 2.0 klx, 16-h day and 8-h night) and low temperature (4 °C, 0.5 klx, 6-h day and 18-h night) growing conditions on hormone-free media has been described. The study explored for the first time the influence of different strategies to store the clones of Betula pubescens and B. pendula var. сarelica (6 genotypes) on the regenerative capacity of collection samples, adaptive potential of regenerated plants and plant production by the in vitro and ex vitro techniques. It was established that both storage strategies provided a persistently high survival rate (82-100%) and regenerative capacity of in vitro shoots (the multiplication coefficient of 4.2-6.3 and rhizogenic activity of 90-100%). The clones retained their characteristics of height growth under the in vitro and ex vitro conditions, and demonstrated intraclonal homogeneity and lack of signs of somaclonal variability. The plants showed substantial interspecific differences at the stage of multiplication and transfer to the greenhouse. The highest percentage of acclimated plants (75-98% depending on the clone genotype) was obtained after planting of micro plants straight in the greenhouse, which simplified the technology and made plant production less costly. long-term in vitro storage, birch, species, genotype, micropropagation, ex vitro adaptation, plant material

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