scholarly journals In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions

2019 ◽  
Vol 43 ◽  
Author(s):  
Tecla dos Santos Silva ◽  
Cristina Ferreira Nepomuceno ◽  
Taliane Leila Soares ◽  
José Raniere Ferreira de Santana
Keyword(s):  
Aerial Part ◽  
Dry Mass ◽  
Growth Conditions ◽  
In Vitro Conservation ◽  
Ex Situ ◽  
Minimal Growth ◽  
Survival Percentage ◽  
Medicinal Substances ◽  
Osmotic Agents

ABSTRACT Poincianella pyramidalis (Tul.) L.P. Queiroz, is an arboreal species endemic to Brazil’s Caatinga biome and an important source of lumber and also of medicinal substances. This study aimed to evaluate the effect of the osmotic agents sucrose, sorbitol and mannitol and the growth retardant paclobutrazol (PBZ) on the induction of slow growth in vitro of P. pyramidalis, seeking to establish alternative techniques for ex situ conservation of the species. In the first experiment, plantlets with seven days of age were inoculated on Woody Plant Medium (WPM) supplemented with four concentrations of sucrose (87.64, 131.46, 175.28 or 219.10 mM) combined with 0.0 or 87.64 mM sorbitol or mannitol. In the second experiment, these plantlets were inoculated on WPM medium with 0.0, 1.5, 3.0, 4.5 or 6.0 µM PBZ and supplemented with 87.64 mM sucrose. For both experiments, the survival percentage of the plants was evaluated every 60 days, and at the end of 240 days, the following parameters were recorded: number of green leaves and senescent leaves; length of the aerial part and longest root; and dry mass of the aerial part and roots. The best results to maintain the quality of the P. pyramidalis plants conserved in vitro were obtained on medium supplemented with 219.10 mM sucrose for up to 240 days without subculture. With respect to PBZ, the concentration of 6.0 μM can be recommended for in vitro conservation of P. pyramidalis for 240 days without the need of subculturing.

scholarly journals In vitro conservation of Amburana cearensis (Fabaceae)

2020 ◽  
Vol 50 (7) ◽  
Author(s):  
Bruno Freitas Matos Alvim ◽  
Ana Valéria Vieira de Souza ◽  
Alone Lima-Brito ◽  
Priscila Tavares Fonseca ◽  
Taliane Leila Soares ◽  
...  
Keyword(s):  
Aerial Part ◽  
Slow Growth ◽  
Plant Genetic Resources ◽  
Dry Mass ◽  
In Vitro Conservation ◽  
Ex Situ ◽  
Storage Process ◽  
Survival Percentage ◽  
Osmotic Agents

ABSTRACT: Slow-growth tissue culture allows in vitro conservation of plant genetic resources and is a complementary technique to conventional preservation methods. The objective of this study was to evaluate the effect of the culture medium supplemented with sucrose, mannitol and sorbitol osmotic agents on the induction of in vitro slow growth of A. cearensis, seeking to establish alternative techniques for ex situ conservation of the species. Plants with age of 10 days were inoculated in woody plant medium (WPM) supplemented with different concentrations of sucrose (87.64, 131.46, 175.28 or 262.92 mM) combined with mannitol or sorbitol at varying concentrations (0.0, 43.8, 65.73, 87.64 or 131.46 mM), solidified with 0.7% agar. The following parameters were evaluated: survival percentage, number of senescent leaves, aerial part length, root length, aerial part dry mass, root dry mass; and number of shoots. The conservation of A. cearensis was viable for up to 300 days in WPM supplemented with 262.92 mM of sucrose or the combination of 87.64 mM of sucrose with 43.80 mM of mannitol, because besides achieving a survival percentage greater than 55.0%, the plants conserved in these media presented normal development and the best appearances, with predominance of uniform green leaves during the storage process.

scholarly journals In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions

2011 ◽  
Vol 46 (4) ◽  
pp. 384-389 ◽  
Author(s):  
Tatiane Loureiro da Silva ◽  
Jonny Everson Scherwinski-Pereira
Keyword(s):  
Low Temperatures ◽  
Culture Medium ◽  
Shoot Growth ◽  
Slow Growth ◽  
Growth Conditions ◽  
In Vitro Cultures ◽  
In Vitro Conservation ◽  
Piper Aduncum ◽  
Osmotic Agents

The objective of this work was to evaluate in vitro storage of Piper aduncum and P. hispidinervum under slow-growth conditions. Shoots were stored at low temperatures (10, 20 and 25°C), and the culture medium was supplemented with osmotic agents (sucrose and mannitol - at 1, 2 and 3%) and abiscisic acid - ABA (0, 0.5, 1.0, 2.0 and 3.0 mg L-1). After six-months of storage, shoots were evaluated for survival and regrowth. Low temperature at 20ºC was effective for the in vitro conservation of P. aduncum and P. hispidinervum shoots. In vitro cultures maintained at 20ºC on MS medium showed 100% survival with slow-growth shoots. The presence of mannitol or ABA, in the culture medium, negatively affected shoot growth, which is evidenced by the low rate of recovered shoots.

scholarly journals In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagation

2017 ◽  
Vol 52 (12) ◽  
pp. 1286-1290 ◽  
Author(s):  
Hugo Teixeira Gomes ◽  
Patrícia Monah Cunha Bartos ◽  
Maíra Teixeira de Andrade ◽  
Raphael Ferreira Almeida ◽  
Luciana Florencio de Lacerda ◽  
...  
Keyword(s):  
Large Scale ◽  
Growth Conditions ◽  
In Vitro Conservation ◽  
Nodal Segments ◽  
Minimal Growth

Abstract: The objective of this work was to evaluate the micropropagation of blackberry (Rubus spp.) cultivars, after in vitro conservation under minimal growth conditions. Nodal segments of the 'Guarani', 'Caingangue', 'Ébano', and 'Xavante' genotypes were conserved under minimal growth conditions at 20ºC, for 15 months. Microshoots were regenerated and multiplied by up to five successive subcultures, when they were rooted and acclimatized. After 30 days of acclimatization in a greenhouse, rooted plantlets showed no significant losses. Blackberry cultivars can be conserved in vitro for 15 months, without subcultures and, after this time, they can be micropropagated on a large-scale, maintaining the regenerative potential and multiplication.

In vitro storage ofXanthosoma spp. under minimal growth conditions

1994 ◽  
Vol 36 (3) ◽  
pp. 309-316 ◽  
Author(s):  
E. A. Zandvoort ◽  
M. J. H. Hulshof ◽  
G. Staritsky
Keyword(s):  
Growth Conditions ◽  
Minimal Growth ◽  
In Vitro Storage

scholarly journals Plant Growth Regulators in the in Vitro Cultivation of Acmella oleracea (L.)

2020 ◽  
Vol 8 (2) ◽  
pp. 774
Author(s):  
Nastassja Kimberlly Lima ◽  
Eloisa Schneider Da Silva ◽  
Rayane Monique Sete Da Cruz ◽  
Pedro Henrique Riboldi Monteiro ◽  
Glacy Jaqueline Da Silva
Keyword(s):  
Growth Regulators ◽  
Root Length ◽  
Aerial Part ◽  
Large Scale ◽  
Dry Mass ◽  
Fresh Mass ◽  
Limiting Factor ◽  
Naphthalene Acetic Acid ◽  
In Vitro Cultivation

Acmella oleracea is a tropical plant, typical of the northern region of Brazil. The species belongs to the Asteraceae family and has great therapeutic, pharmacological and industrial potential. A limiting factor for the production of this species on a large scale is the short life cycle. The tissue culture programs use synthetic hormones based on cytokinins, such as kinetin and benzylaminopurine (BAP) and auxins such as naphthalene acetic acid (ANA). The objective of this research was to evaluate the effect of growth regulators on the production of Acmella oleracea "in vitro". The experimental test was carried out with control (C), without the addition of growth regulators and five treatments, composed of: (T1) 0.1; (T2) 0.3; (T3) 0.5 mg L-1 kinetin; (T4) 0.1 mg L-1 of BAP and ANA; (T5) 0.5 mg L-1 of BAP and ANA. The experimental design was a completely randomized block in a factorial arrangement with six treatments, three blocks and twenty-five repetitions per block. The evaluated parameters were: germination, root formation, aerial part length, root length, aerial part fresh mass and root fresh mass, aerial part dry mass and root dry mass. The data obtained were subjected to analysis of variance (p <0.05) and compared using the Tukey test. The results showed that kinetin positively contributed to seed germination and aerial part dry mass development. Treatment 1 had the best results for the parameters root length, shoot length and root dry mass.

Minimal growth in vitro conservation of coffee (Coffea spp.)

1991 ◽  
Vol 27 (3) ◽  
pp. 333-339 ◽  
Author(s):  
Anna Bertrand-Desbrunais ◽  
Michel Noirot ◽  
Andr� Charrier
Keyword(s):  
In Vitro Conservation ◽  
Minimal Growth
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