scholarly journals In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

10.3791/55975 ◽  
2017 ◽  
Author(s):  
Daisuke Kurihara ◽  
Yusuke Kimata ◽  
Tetsuya Higashiyama ◽  
Minako Ueda
Keyword(s):  
Arabidopsis Thaliana ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Embryo Patterning

A new visible-light-excitable ICT-CHEF-mediated fluorescence ‘turn-on’ probe for the selective detection of Cd2+ in a mixed aqueous system with live-cell imaging

2015 ◽  
Vol 44 (12) ◽  
pp. 5763-5770 ◽  
Author(s):  
Shyamaprosad Goswami ◽  
Krishnendu Aich ◽  
Sangita Das ◽  
Chitrangada Das Mukhopadhyay ◽  
Deblina Sarkar ◽  
...  
Keyword(s):  
Visible Light ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Aqueous System ◽  
Live Cell ◽  
Selective Detection ◽  
Turn On ◽  
Fluorescence Turn On

A new quinoline based sensor was developed and applied for the selective detection of Cd2+ both in vitro and in vivo.

scholarly journals tdLanYFP, a yellow, bright, photostable and pH insensitive fluorescent protein for live cell imaging and FRET-based sensing strategies

2021 ◽  
Author(s):  
Y. Bousmah ◽  
H. Valenta ◽  
G. Bertolin ◽  
U. Singh ◽  
V. Nicolas ◽  
...  
Keyword(s):  
Single Molecule ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Yellow Fluorescent Protein ◽  
Resonance Energy ◽  
Live Cell ◽  
Live Cells

AbstractYellow fluorescent proteins (YFP) are widely used as optical reporters in Förster Resonance Energy Transfer (FRET) based biosensors. Although great improvements have been done, the sensitivity of the biosensors is still limited by the low photostability and the poor fluorescence performances of YFPs at acidic pHs. In fact, today, there is no yellow variant derived from the EYFP with a pK1/2 below ∼5.5. Here, we characterize a new yellow fluorescent protein, tdLanYFP, derived from the tetrameric protein from the cephalochordate B. lanceolatum, LanYFP. With a quantum yield of 0.92 and an extinction coefficient of 133 000 mol−1.L.cm−1, it is, to our knowledge, the brightest dimeric fluorescent protein available, and brighter than most of the monomeric YFPs. Contrasting with EYFP and its derivatives, tdLanYFP has a very high photostability in vitro and preserves this property in live cells. As a consequence, tdLanYFP allows the imaging of cellular structures with sub-diffraction resolution with STED nanoscopy. We also demonstrate that the combination of high brightness and strong photostability is compatible with the use of spectro-microscopies in single molecule regimes. Its very low pK1/2 of 3.9 makes tdLanYFP an excellent tag even at acidic pHs. Finally, we show that tdLanYFP can be a FRET partner either as donor or acceptor in different biosensing modalities. Altogether, these assets make tdLanYFPa very attractive yellow fluorescent protein for long-term or single-molecule live-cell imaging that is also suitable for FRET experiment including at acidic pH.

Live-Cell Imaging of F-Actin Dynamics During Fertilization in Arabidopsis thaliana

2017 ◽  
pp. 47-54 ◽  
Author(s):  
Daichi Susaki ◽  
Daisuke Maruyama ◽  
Ramesh Yelagandula ◽  
Frederic Berger ◽  
Tomokazu Kawashima
Keyword(s):  
Arabidopsis Thaliana ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Actin Dynamics

scholarly journals The Use of Live Cell Imaging and Automated Image Analysis to Assist With Determining Optimal Parameters for Angiogenic Assay in vitro

2019 ◽  
Vol 7 ◽  
Author(s):  
Brooke M. Huuskes ◽  
Ryan J. DeBuque ◽  
Peter G. Kerr ◽  
Chrishan S. Samuel ◽  
Sharon D. Ricardo
Keyword(s):  
Image Analysis ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Automated Image Analysis ◽  
Optimal Parameters

Naked-eye detection of Pd2+ ion using a highly selective fluorescent heterocyclic probe by “turn-off” response and in-vitro live cell imaging

2020 ◽  
Vol 394 ◽  
pp. 112441
Author(s):  
Satyajit Mahata ◽  
Araghni Bhattacharya ◽  
Jadi Praveen Kumar ◽  
Biman B. Mandal ◽  
Vadivelu Manivannan
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Eye Detection ◽  
Naked Eye ◽  
Naked Eye Detection

scholarly journals Live-cell imaging of nuclear–chromosomal dynamics in bovine in vitro fertilised embryos

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Tatsuma Yao ◽  
Rie Suzuki ◽  
Natsuki Furuta ◽  
Yuka Suzuki ◽  
Kyoko Kabe ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

An aggregation-induced phosphorescence probe for calcium ion-specific detection and live-cell imaging in Arabidopsis thaliana

2019 ◽  
Vol 55 (33) ◽  
pp. 4841-4844 ◽  
Author(s):  
Guilin Chen ◽  
Zaicai Zhou ◽  
Hui Feng ◽  
Chenyan Zhang ◽  
Yifan Wang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Calcium Ion ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Molecular Probe ◽  
Specific Detection ◽  
Phosphorescence Probe

A molecular probe with aggregation-induced phosphorescence (AIP) properties for calcium ion-specific detection and imaging in vivo was designed.

scholarly journals Live Cell Imaging of In Vitro Human Trophoblast Syncytialization1

2014 ◽  
Vol 90 (6) ◽  
Author(s):  
Rui Wang ◽  
Yan-Li Dang ◽  
Ru Zheng ◽  
Yue Li ◽  
Weiwei Li ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Human Trophoblast
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