Targeted Mutagenesis in Hexaploid Bread Wheat Using the TALEN and CRISPR/Cas Systems

2017 ◽  
pp. 169-185 ◽  
Author(s):  
Yanpeng Wang ◽  
Yuan Zong ◽  
Caixia Gao
Keyword(s):  
Bread Wheat ◽  
Targeted Mutagenesis

Influence of yellow rust on quality indicators of bread wheat grain (t. Aestivuml.)

2019 ◽  
Vol 326 (1) ◽  
pp. 158-161
Author(s):  
G.M. Hasanova ◽  
◽  
Kh.N. Rustamov ◽  
Keyword(s):  
Bread Wheat ◽  
Quality Indicators ◽  
Yellow Rust ◽  
Wheat Grain

Assessment Of Heterosis For Morphological And Yield Donating Traits In Bread Wheat By Line X Tester Analysis.

2019 ◽  
Vol 02 (09) ◽  
pp. 23-39
Author(s):  
Syed Bilal Hussain ◽  
◽  
Sadaf Zahra ◽  
Ali Bakhsh ◽  
Muhammad Zubair ◽  
...  
Keyword(s):  
Bread Wheat

Molecular Basis of Iterative C–H Oxidation by TamI, a Multifunctional P450 Monooxygenase from the Tirandamycin Biosynthetic Pathway

2020 ◽  
Author(s):  
Sean A. Newmister ◽  
Kinshuk Raj Srivastava ◽  
Rosa V. Espinoza ◽  
Kersti Caddell Haatveit ◽  
Yogan Khatri ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Molecular Dynamics Simulations ◽  
Molecular Basis ◽  
Hydrophobic Interactions ◽  
Cytochromes P450 ◽  
Targeted Mutagenesis ◽  
P450 Monooxygenase ◽  
Bond Functionalization ◽  
Dynamics Simulations

Biocatalysis offers an expanding and powerful strategy to construct and diversify complex molecules by C-H bond functionalization. Due to their high selectivity, enzymes have become an essential tool for C-H bond functionalization and offer complementary reactivity to small-molecule catalysts. Hemoproteins, particularly cytochromes P450, have proven effective for selective oxidation of unactivated C-H bonds. Previously, we reported the in vitro characterization of an oxidative tailoring cascade in which TamI, a multifunctional P450 functions co-dependently with the TamL flavoprotein to catalyze regio- and stereoselective hydroxylations and epoxidation to yield tirandamycin A and tirandamycin B. TamI follows a defined order including 1) C10 hydroxylation, 2) C11/C12 epoxidation, and 3) C18 hydroxylation. Here we present a structural, biochemical, and computational investigation of TamI to understand the molecular basis of its substrate binding, diverse reactivity, and specific reaction sequence. The crystal structure of TamI in complex with tirandamycin C together with molecular dynamics simulations and targeted mutagenesis suggest that hydrophobic interactions with the polyene chain of its natural substrate are critical for molecular recognition. QM/MM calculations and molecular dynamics simulations of TamI with variant substrates provided detailed information on the molecular basis of sequential reactivity, and pattern of regio- and stereo-selectivity in catalyzing the three-step oxidative cascade.<br>

Exogenous sodium nitroprusside increases antioxidative potential and grain yield of bread wheat exposed to cadmium

2018 ◽  
Vol 51 (2) ◽  
Author(s):  
Muhammad Arshad ◽  
Muhammad Iqbal ◽  
Muhammad Yasin Ashraf ◽  
Qasim Ali ◽  
Naeem Iqbal
Keyword(s):  
Grain Yield ◽  
Sodium Nitroprusside ◽  
Bread Wheat
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