Quantitation of Surface Coating on Nanoparticles Using Thermogravimetric Analysis

Author(s):  
Alpana A. Dongargaonkar ◽  
Jeffrey D. Clogston
1987 ◽  
Vol 58 (04) ◽  
pp. 1064-1067 ◽  
Author(s):  
K Kodama ◽  
B Pasche ◽  
P Olsson ◽  
J Swedenborg ◽  
L Adolfsson ◽  
...  

SummaryThe mode of F Xa inhibition was investigated on a thromboresistant surface with end-point attached partially depoly-merized heparin of an approximate molecular weight of 8000. Affinity chromatography revealed that one fourth of the heparin used in surface coating had high affinity for antithrombin III (AT). The heparin surface adsorbed AT from both human plasma and solutions of purified AT. By increasing the ionic strength in the AT solution the existence of high and low affinity sites could be shown. The uptake of AT was measured and the density of available high and low affinity sites was found to be in the range of 5 HTid 11 pic.omoles/cmf, respectively Thus the estimated density of biologically active high and low ailmity heparm respectively would be 40 and 90 ng/cm2 The heparin coating did not take up or exert F Xa inhibition by itself. With AT adsorbed on both high and low affinity heparin the surface had the capacity to inhibit several consecutive aliquots of F Xa exposed to the surface. When mainly high affinity sites were saturated with AT the inhibition capacity was considerably lower. Tt was demonstrated that the density of AT on both high and low affinity heparin determines the F Xa inhibition capacity whereas the amount of AT on high affinity sites limits the rate of the reaction. This implies that during the inhibition of F Xa there is a continuous surface-diffusion of AT from sites of a lower class to the high affinity sites where the F Xa/AT complex is formed and leaves the surface. The ability of the immobilized heparin to catalyze inhibition of F Xa is likely to be an important component for the thromboresistant properties of a heparin coating with non-compromized AT binding sequences.


2013 ◽  
Vol 51 (2) ◽  
pp. 137-144
Author(s):  
Naesung Lee ◽  
Jeung Choon Goak ◽  
Tae Yang Kim ◽  
Jongwan Jung ◽  
Young-Soo Seo ◽  
...  

2004 ◽  
Vol 11 (2) ◽  
pp. 133-150 ◽  
Author(s):  
M. B. Dizon ◽  
J. Yang ◽  
F. B. Cheung ◽  
J. L. Rempe ◽  
K. Y. Suh ◽  
...  

2019 ◽  
Vol 68 (2) ◽  
Author(s):  
Eugenio Pedullà ◽  
Gianluca Plotino ◽  
Mauro Scibilia ◽  
Nicola M. Grande ◽  
Daniele De Santis ◽  
...  

2016 ◽  
Vol 10 (4) ◽  
pp. 465-472 ◽  
Author(s):  
Debora Almeida ◽  
◽  
Maria de Fatima Marques ◽  

In the present work, the pyrolysis of polypropylene and polyethylene was evaluated with and without the addition of niobium oxide as catalyst by means of thermogravimetric analysis and experiments in a glass reactor. The results revealed that niobium oxide performed well in the pyrolysis of both polypropylene and polyethylene separately. For the mixture of polypropylene with polyethylene, the catalyst reduced the pyrolysis time.


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