Isolation and Purification of Villous Cytotrophoblast Cells from Term Human Placenta

2017 ◽  
pp. 219-231 ◽  
Author(s):  
Hélène Clabault ◽  
Laetitia Laurent ◽  
J. Thomas Sanderson ◽  
Cathy Vaillancourt
Keyword(s):  
Human Placenta ◽  
Isolation And Purification ◽  
Cytotrophoblast Cells

The human placenta expresses transcription enhancer factor-1 but there is no correlation with the expression of placental lactogen

1996 ◽  
Vol 16 (2) ◽  
pp. 205-210 ◽  
Author(s):  
G Quinn ◽  
D S W Boam ◽  
J R E Davis ◽  
J D Glazier ◽  
P Mylona ◽  
...  
Keyword(s):  
Human Placenta ◽  
First Trimester ◽  
Placental Lactogen ◽  
Transcriptional Enhancer ◽  
Term Placenta ◽  
Consensus Binding Sequence ◽  
Cytotrophoblast Cells ◽  
Binding Sequence ◽  
Transcription Enhancer ◽  
Enhancer Factor

ABSTRACT A transcriptional enhancer which has a consensus binding sequence for transcription enhancer factor-1 (TEF-1) has been found 3′ of the hPL3 gene. We examined whether TEF-1 is expressed by the human placenta and whether such expression is co-ordinated with that of human placental lactogen (hPL). Probing Northern blots of total RNA from first trimester and term placenta, the choriocarcinoma-derived cell line JAr and primary cultured cytotrophoblast cells with a cDNA for TEF-1 revealed transcripts of 12–13 kb and 3–4 kb. The level of TEF-1 expression was the same in first trimester as compared with term placenta and in undifferentiated JAr as compared with differentiated cytotrophoblast cells. hPL expression was tenfold higher in term compared with first trimester placenta and, whilst detectable in cytotrophoblast cells, was undetectable in JAr cells. These data show that TEF-1 is expressed by the placenta but is not co-ordinated with hPL expression.

Isolation and purification of protease from human placenta by foam fractionation

1987 ◽  
Vol 29 (8) ◽  
pp. 934-940 ◽  
Author(s):  
P. Sarkar ◽  
P. Bhattacharya ◽  
R. N. Mukherjea ◽  
M. Mukherjea
Keyword(s):  
Human Placenta ◽  
Foam Fractionation ◽  
Isolation And Purification

scholarly journals The effect of human placenta cytotrophoblast cells on the maturation and T cell stimulating ability of dendritic cells in vitro

2010 ◽  
Vol 162 (1) ◽  
pp. 91-99 ◽  
Author(s):  
V. Yu. Talayev ◽  
A. V. Matveichev ◽  
M. A. Lomunova ◽  
M. V. Talayeva ◽  
M. E. Tsaturov ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Cell ◽  
Human Placenta ◽  
Cytotrophoblast Cells

207. The expression of caspase-14 in the human placenta

2005 ◽  
Vol 17 (9) ◽  
pp. 79
Author(s):  
A. Charles ◽  
S. Hisheh ◽  
D. W. R. Kam ◽  
A. M. Dharmarajan
Keyword(s):  
Human Placenta ◽  
First Trimester ◽  
Explant Culture ◽  
Control Group ◽  
Term Placenta ◽  
Protein Levels ◽  
Significant Difference ◽  
Cytotrophoblast Cells ◽  
Immunohistochemical Studies

Pro-apoptotic genes have a role in the differentiation process in the placenta leading to the fusion of cytotrophoblast cells to form the protective syncytiotrophoblast layer. The mechanisms of apoptosis in the human placenta are not clearly understood. However, a major placental apoptotic-signalling pathway is known to involve the caspases. Caspase-14 is the most recently discovered member of the caspase family members and has not previously been examined in the human placenta. The aim of the present study was to detect caspase-14 in the human placenta and study its role in apoptosis. Human placentae were collected from first trimester and term gestation. The study consisted of two parts. In the first part, first trimester and term placentae were assessed for caspase-14 by western blotting and mRNA analysis and localised with immunohistochemical studies. In the second part, apoptosis in first trimester placenta was inhibited in an in-vitro model of explant villi culture with superoxide dismutase (SOD) treatment and the genes assessed. The first study demonstrated caspase-14 to be a cytoplasmic protein localised in the cytotrophoblast cells, the mesenchyme and in the syncytiotrophoblast of the first trimester. In the term placenta, caspase-14 was expressed weakly in the syncytiotrophoblast. The immunostaining data suggest a higher expression of caspase-14 in the first trimester compared to the term placenta, and this observation was later confirmed by western blot analysis. Using the SOD in-vitro explant culture model, no significant difference in the caspase-14 protein levels were seen in either the SOD or control group. This novel study demonstrates for the first time that caspase-14 protein and mRNA are present in the human placenta. The function of caspase-14 in the human placenta is unclear.

scholarly journals OVO-like 1 regulates progenitor cell fate in human trophoblast development

2015 ◽  
Vol 112 (45) ◽  
pp. E6175-E6184 ◽  
Author(s):  
Stephen J. Renaud ◽  
Damayanti Chakraborty ◽  
Clifford W. Mason ◽  
M. A. Karim Rumi ◽  
Jay L. Vivian ◽  
...  
Keyword(s):  
Cell Fate ◽  
Human Placenta ◽  
Progenitor Cell ◽  
Trophoblast Cell ◽  
Fetal Blood ◽  
Trophoblast Cells ◽  
Human Trophoblast ◽  
Barrier Integrity ◽  
Cytotrophoblast Cells ◽  
Stimulation Of

Epithelial barrier integrity is dependent on progenitor cells that either divide to replenish themselves or differentiate into a specialized epithelium. This paradigm exists in human placenta, where cytotrophoblast cells either propagate or undergo a unique differentiation program: fusion into an overlying syncytiotrophoblast. Syncytiotrophoblast is the primary barrier regulating the exchange of nutrients and gases between maternal and fetal blood and is the principal site for synthesizing hormones vital for human pregnancy. How trophoblast cells regulate their differentiation into a syncytium is not well understood. In this study, we show that the transcription factor OVO-like 1 (OVOL1), a homolog of Drosophila ovo, regulates the transition from progenitor to differentiated trophoblast cells. OVOL1 is expressed in human placenta and was robustly induced following stimulation of trophoblast differentiation. Disruption of OVOL1 abrogated cytotrophoblast fusion and inhibited the expression of a broad set of genes required for trophoblast cell fusion and hormonogenesis. OVOL1 was required to suppress genes that maintain cytotrophoblast cells in a progenitor state, including MYC, ID1, TP63, and ASCL2, and bound specifically to regions upstream of each of these genes. Our results reveal an important function of OVOL1 as a regulator of trophoblast progenitor cell fate during human trophoblast development.

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