Using Nanodiscs to Probe Ca2+-Dependent Membrane Interaction of Synaptotagmin-1

2018 ◽  
pp. 221-236 ◽  
Author(s):  
Ekaterina Stroeva ◽  
Shyam S. Krishnakumar
Keyword(s):  
Membrane Interaction ◽  
Synaptotagmin 1

scholarly journals Synaptotagmin-1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domain

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Clémence Gruget ◽  
Oscar Bello ◽  
Jeff Coleman ◽  
Shyam S. Krishnakumar ◽  
Eric Perez ◽  
...  
Keyword(s):  
Neurotransmitter Release ◽  
Mutational Analysis ◽  
Membrane Binding ◽  
Surface Force ◽  
Membrane Interaction ◽  
C2 Domains ◽  
Binding Energetics ◽  
Synaptotagmin 1 ◽  
Site Binding ◽  
C2a Domain

Abstract Synaptotagmin interaction with anionic lipid (phosphatidylserine/phosphatidylinositol) containing membranes, both in the absence and presence of calcium ions (Ca2+), is critical to its central role in orchestrating neurotransmitter release. The molecular surfaces involved, namely the conserved polylysine motif in the C2B domain and Ca2+-binding aliphatic loops on both C2A and C2B domains, are known. Here we use surface force apparatus combined with systematic mutational analysis of the functional surfaces to directly measure Syt1-membrane interaction and fully map the site-binding energetics of Syt1 both in the absence and presence of Ca2+. By correlating energetics data with the molecular rearrangements measured during confinement, we find that both C2 domains cooperate in membrane binding, with the C2B domain functioning as the main energetic driver, and the C2A domain acting as a facilitator.

scholarly journals Non-Native Metal Ions Reveal the Role of Electrostatics in Synaptotagmin 1-Membrane Interaction

2017 ◽  
Vol 112 (3) ◽  
pp. 393a
Author(s):  
Sachin Katti ◽  
Atul Srivastava ◽  
Bin Her ◽  
Alexander B. Taylor ◽  
Tatyana Igumenova
Keyword(s):  
Metal Ions ◽  
Native Metal ◽  
Membrane Interaction ◽  
Synaptotagmin 1

Molecular basis of actin-membrane interaction.

1990 ◽  
Vol 30 (3) ◽  
pp. 127-132
Author(s):  
Shoichiro TSUKITA ◽  
Sachiko TSUKITA
Keyword(s):  
Molecular Basis ◽  
Membrane Interaction

scholarly journals Theory of cell membrane interaction with glass

2021 ◽  
Vol 103 (3) ◽  
Author(s):  
Richard W. Clarke
Keyword(s):  
Cell Membrane ◽  
Membrane Interaction
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