Recombinant Methioninase as a DNA Demethylation Agent

2019 ◽  
pp. 279-284
Author(s):  
Robert M. Hoffman ◽  
David Machover
Keyword(s):  
Dna Demethylation ◽  
Demethylation Agent

scholarly journals 5-Azacytidine Increases Tanshinone Production in Salvia miltiorrhiza Hairy Roots Through Epigenetic Modulation

2021 ◽  
Author(s):  
Bo-Cheng Yang ◽  
Meng-Shiou Lee ◽  
Ming-Kuem Lin ◽  
Wen-Te Chang
Keyword(s):  
Secondary Metabolites ◽  
Hairy Roots ◽  
Salvia Miltiorrhiza ◽  
Methylation Pattern ◽  
Dna Demethylation ◽  
Upward Trend ◽  
Promoter Regions ◽  
Medicinal Crop ◽  
Epigenetic Modulation ◽  
Demethylation Agent

Abstract Recent studies have indicated strong connections between epigenetic modulation and secondary metabolites in plants. It is vital to understand the roles of epigenetics in the production of secondary metabolites. In this study, the DNA demethylation agent 5-azacytidine (5-Az) was used on the hairy roots of the medicinal crop Salvia miltiorrhiza to investigate its effect on secondary metabolite production, gene expression, and methylation levels in gDNA and promoter regions. Our results showed that the contents of tanshinones in S. miltiorrhiza hairy roots increased by 1.5 to 5 times, and most genes in the MEP pathway showed an upward trend. According to our NGS analysis, the methylation pattern in the copalyl diphosphate synthase (CPS) promoter was altered, and 51 out of 145 cytosines were demethylated during 5-Az treatment. A total of 36 different transcription factors (TFs) were identified in these demethylation sites. Among these TFs, NF-Y and MYB were frequently found in our results. This is the first report to demonstrate a possible mechanism of DNA methylation participating in tanshinone biosynthesis in S. miltiorrhiza hairy roots by modulating the CPS promoter and TFs.

scholarly journals DNA demethylation agent 5azadC downregulates HPV16 E6 expression in cervical cancer cell lines independently of TBX2 expression

2019 ◽  
Author(s):  
Jerome Perrard ◽  
Adrien Morel ◽  
Koceila Meznad ◽  
Philippe Paget‑Bailly ◽  
Veronique Dalstein ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Dna Demethylation ◽  
Cervical Cancer Cell ◽  
Hpv16 E6 ◽  
Tbx2 Expression ◽  
Demethylation Agent

DNA Demethylation Regulates Anabolic and Catabolic Gene Expression in Intervertebral Disc Cells

2014 ◽  
Vol 4 (1_suppl) ◽  
pp. s-0034-1376587-s-0034-1376587
Author(s):  
N. Chutkan ◽  
R. Sangani ◽  
H. Zhou ◽  
S. Fulzele
Keyword(s):  
Gene Expression ◽  
Intervertebral Disc ◽  
Dna Demethylation ◽  
Catabolic Gene ◽  
Disc Cells

Genome-wide reprogramming by DNA demethylation during mouse oocyte growth and early development

2014 ◽  
Author(s):  
Akihiko Sakashita ◽  
Yosuke Iseki ◽  
Mei Nakajima ◽  
Takuya Wakai ◽  
Hisato Kobayashi ◽  
...  
Keyword(s):  
Early Development ◽  
Dna Demethylation ◽  
Mouse Oocyte ◽  
Oocyte Growth ◽  
Genome Wide
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