A Simple and Rapid Method for Determining Transgenic Cotton Plants Using a Marker Gene

2018 ◽  
pp. 187-194
Author(s):  
Baohong Zhang ◽  
Hongmei Wang ◽  
Fang Liu ◽  
Qinglian Wang
Keyword(s):  
Rapid Method ◽  
Marker Gene ◽  
Transgenic Cotton ◽  
Cotton Plants

A Simple and Rapid Method for Determining Transgenic Cotton Plants

2012 ◽  
pp. 153-160
Author(s):  
Baohong Zhang ◽  
Hongmei Wang ◽  
Fang Liu ◽  
Qinglian Wang
Keyword(s):  
Rapid Method ◽  
Transgenic Cotton ◽  
Cotton Plants

Co-overexpression of AVP1 and AtNHX1 in Cotton Further Improves Drought and Salt Tolerance in Transgenic Cotton Plants

2014 ◽  
Vol 33 (2) ◽  
pp. 167-177 ◽  
Author(s):  
Guoxin Shen ◽  
Jia Wei ◽  
Xiaoyun Qiu ◽  
Rongbin Hu ◽  
Sundaram Kuppu ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Transgenic Cotton ◽  
Drought And Salt Tolerance ◽  
Cotton Plants

scholarly journals Influence of Cry1Ac Toxin From Bt Cotton on the Soil Microbiota

2019 ◽  
Vol 11 (4) ◽  
pp. 364
Author(s):  
Marcos Gino Fernandes ◽  
Renata Pires de Araújo ◽  
Eduardo Neves Costa ◽  
Ana Claudia Terumi Abe Zangirolymo ◽  
Rodrigo Matheus Pereira
Keyword(s):  
Soil Bacteria ◽  
Marker Gene ◽  
First Record ◽  
Transgenic Cotton ◽  
Natural Soil ◽  
Bt Cotton ◽  
Bacterial Populations ◽  
Soil Microbiota ◽  
Cry1ac Toxin ◽  
Gene 16S Rrna

The first record of transgenic cotton cultivation in Brazil was in 2005, of that of the cultivar MON 531, possessing the cry1Ac gene. Since then, no evaluation has been performed to understand whether the cultivation of Bt cotton has caused any interference with the soil microbiota, including bacteria. In this context, our research was aimed to assess whether the cultivation of Bt cotton negatively affects the community of soil bacteria, through quantitative and metagenomic analyses (marker gene 16S rRNA) for phylum identification. Samples of bacterial populations obtained from the soil cultivated with Bt cotton expressing the Cry1Ac toxin were compared with soil samples from the area cultivated with conventional cotton. Significant differences were not observed in the measure of colony-forming units of bacteria between the soils cultivated with Bt and non-Bt cotton; however, differences were detected only when comparing samples from different collection times of the Bt treatment. Cultivation of Bt cotton did not affect the diversity of the soil bacterial population. Overall, our study shows that, similar to most of the works that have been reported worldwide, cultivation of transgenic cotton does not seem to affect the quantity and diversity of natural soil bacteria.

Functional Analysis of Nodulin-like Promoter in Transgenic Cotton Plants

2005 ◽  
Vol 47 (10) ◽  
pp. 1254-1259 ◽  
Author(s):  
Mao-Zhi REN ◽  
Quan-Jia CHEN ◽  
Li LI ◽  
Rui ZHANG ◽  
San-Dui GUO
Keyword(s):  
Functional Analysis ◽  
Transgenic Cotton ◽  
Cotton Plants

Expression and inheritance of chimeric cry2AX1 gene in transgenic cotton plants generated through somatic embryogenesis

2015 ◽  
Vol 51 (4) ◽  
pp. 379-389 ◽  
Author(s):  
A. R. Sakthi ◽  
A. Naveenkumar ◽  
P. S. Deepikha ◽  
N. Balakrishnan ◽  
K. K. Kumar ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Transgenic Cotton ◽  
Cotton Plants

Enhanced resistance against Thielaviopsis basicola in transgenic cotton plants expressing Arabidopsis NPR1 gene

2012 ◽  
Vol 22 (2) ◽  
pp. 359-368 ◽  
Author(s):  
Vinod Kumar ◽  
Sameer G. Joshi ◽  
Alois A. Bell ◽  
Keerti S. Rathore
Keyword(s):  
Transgenic Cotton ◽  
Thielaviopsis Basicola ◽  
Enhanced Resistance ◽  
Cotton Plants ◽  
Npr1 Gene

Influences of root exudates of bivalent transgenic cotton plants on defense proteins and the growth of conventional parental cotton (Zhong 1423-6)

2015 ◽  
Vol 16 (4) ◽  
pp. 787
Author(s):  
Xue Shi ◽  
Hong-Sheng Wu ◽  
Ji Li ◽  
Qian-Qi Ren ◽  
Ming-Yan Wang ◽  
...  
Keyword(s):  
Root Exudates ◽  
Transgenic Cotton ◽  
Defense Proteins ◽  
Cotton Plants

Polymerase Chain Reaction Detection of the Modified cry1Ac Gene in Transgenic Bt (Bollgard®) Cotton

2005 ◽  
Vol 40 (2) ◽  
pp. 206-210
Author(s):  
Yu Cheng Zhu ◽  
John J. Adamczyk
Keyword(s):  
Polymerase Chain Reaction ◽  
Transgenic Cotton ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Cry1ac Gene ◽  
Future Studies ◽  
Cotton Plants ◽  
Dna Fragment ◽  
Polymerase Chain ◽  
Bacterium Bacillus

Transgenic cotton (Gossypium hirsutum L.) containing a modified cry1Ac gene from the soil bacterium Bacillus thuringiensis Berliner has been widely adopted for suppression of lepidopterous pests. As of 2004, over 90% of the cotton acreage in the mid-southern United States contained this modified cry1Ac gene. We developed a technique using the polymerase chain reaction (PCR) for routine detection of the cry1Ac gene in transgenic cotton plants. A total of eight cry1Ac genes were aligned for the PCR primer design. A DNA fragment was amplified from transgenic cotton, sequenced, and confirmed to be a portion of the cry1Ac gene. A total of 150 cotton plants representing four cultivars were examined for the presence of the cry1Ac gene. Results demonstrated that all of these cotton plants harbored the cry1Ac gene (i.e., 100% purity). This PCR technique can be used for future studies involving the expression of cry1Ac gene as well as corresponding protein expression.

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