scholarly journals Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction

2019 ◽  
pp. 1-27 ◽  
Author(s):  
Daniel R. Crooks ◽  
Teresa W.-M. Fan ◽  
W. Marston Linehan
Keyword(s):  
Tissue Culture ◽  
Stable Isotope ◽  
Mammalian Cells ◽  
Metabolic Labeling ◽  
Sample Extraction

Stable isotope metabolic labeling of a mouse model reveals synaptic biomarkers for anxiety disorders

2009 ◽  
Vol 42 (05) ◽  
Author(s):  
MD Filiou ◽  
YY Zhang ◽  
B Bisle ◽  
E Frank ◽  
MS Kessler ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Mouse Model ◽  
Anxiety Disorders ◽  
Metabolic Labeling

scholarly journals Compartment Modeling for Mammalian Protein Turnover Studies by Stable Isotope Metabolic Labeling

2012 ◽  
Vol 84 (9) ◽  
pp. 4014-4021 ◽  
Author(s):  
Shenheng Guan ◽  
John C. Price ◽  
Sina Ghaemmaghami ◽  
Stanley B. Prusiner ◽  
Alma L. Burlingame
Keyword(s):  
Stable Isotope ◽  
Protein Turnover ◽  
Metabolic Labeling ◽  
Compartment Modeling ◽  
Mammalian Protein

Metabolic Co-Operation Between Biochemically Marked Mammalian Cells in Tissue Culture

1969 ◽  
Vol 4 (2) ◽  
pp. 353-367
Author(s):  
H. SUBAK-SHARPE ◽  
R. R. BÜRK ◽  
J. D. PITTS
Keyword(s):  
Tissue Culture ◽  
Nucleic Acid ◽  
Mixed Culture ◽  
Molecular Basis ◽  
Mammalian Cells ◽  
Genetic Variant ◽  
Cell Contact ◽  
Metabolic Function ◽  
Indirect Contact ◽  
Fibroblast Line

Cells of a genetic variant of the hamster fibroblast line BHK 21 which lack inosinic pyrophosphorylase activity (IPP- cells) and therefore cannot normally incorporate [3H]hypoxanthine were grown in mixed culture with cells of BHK 21 sublines which have inosinic pyrophosphorylase activity (IPP+ cells). If not in contact with IPP+ cells, IPP- cells do not incorporate added [3H]hypoxanthine into nucleic acid. IPP+ cells always do incorporate [3H]hypoxanthine and IPP- cells when in direct or indirect contact with IPP+ cells also incorporate the isotope. Cell to cell contact appears to be essential for this gain of a metabolic function by IPP- cells. The possible molecular basis and general implications of the phenomenon are discussed.

scholarly journals Effect of sodium butyrate on lymphocyte activation

1976 ◽  
Vol 144 (6) ◽  
pp. 1674-1678 ◽  
Author(s):  
D Kyner ◽  
P Zabos ◽  
J Christman ◽  
G Acs
Keyword(s):  
Tissue Culture ◽  
Dna Synthesis ◽  
Sodium Butyrate ◽  
Cell Morphology ◽  
Mammalian Cells ◽  
Lymphocyte Activation ◽  
Extended Period ◽  
Present Evidence ◽  
Growth Of A Variety ◽  
Low Concentrations

Butyrate, in relatively low concentrations, has been shown to induce synthesis of enzymes, cause changes in cell morphology, and inhibit growth of a variety of mammalian cells in tissue culture (reviewed in [1]). In this communication, we report our observations on the effect of butyrate on lymphocyte activation. Butyrate completely and reversibly inhibits mitogen-induced blast formation. We present evidence that it does not interfere with the binding of mitogens, that it does not inhibit a number of the early reactions involved in activation, and that it does not affect ongoing DNA synthesis for an extended period of time. However, butyrate rapidly inhibits any increase in the rate of DNA synthesis.

scholarly journals Live-cell vibrational imaging of choline metabolites by stimulated Raman scattering coupled with isotope-based metabolic labeling

The Analyst ◽  
2014 ◽  
Vol 139 (10) ◽  
pp. 2312-2317 ◽  
Author(s):  
Fanghao Hu ◽  
Lu Wei ◽  
Chaogu Zheng ◽  
Yihui Shen ◽  
Wei Min
Keyword(s):  
High Resolution ◽  
Raman Scattering ◽  
Mammalian Cells ◽  
Stimulated Raman Scattering ◽  
Primary Neurons ◽  
High Resolution Imaging ◽  
Metabolic Labeling ◽  
C Elegans ◽  
Resolution Imaging

High-resolution imaging of choline metabolites in living mammalian cells, primary neurons andC. eleganshas been demonstrated with the potential forin vivodisease detection and developmental monitoring.

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