Metabolic Co-Operation Between Biochemically Marked Mammalian Cells in Tissue Culture

1969 ◽  
Vol 4 (2) ◽  
pp. 353-367
Author(s):  
H. SUBAK-SHARPE ◽  
R. R. BÜRK ◽  
J. D. PITTS
Keyword(s):  
Tissue Culture ◽  
Nucleic Acid ◽  
Mixed Culture ◽  
Molecular Basis ◽  
Mammalian Cells ◽  
Genetic Variant ◽  
Cell Contact ◽  
Metabolic Function ◽  
Indirect Contact ◽  
Fibroblast Line

Cells of a genetic variant of the hamster fibroblast line BHK 21 which lack inosinic pyrophosphorylase activity (IPP- cells) and therefore cannot normally incorporate [3H]hypoxanthine were grown in mixed culture with cells of BHK 21 sublines which have inosinic pyrophosphorylase activity (IPP+ cells). If not in contact with IPP+ cells, IPP- cells do not incorporate added [3H]hypoxanthine into nucleic acid. IPP+ cells always do incorporate [3H]hypoxanthine and IPP- cells when in direct or indirect contact with IPP+ cells also incorporate the isotope. Cell to cell contact appears to be essential for this gain of a metabolic function by IPP- cells. The possible molecular basis and general implications of the phenomenon are discussed.

scholarly journals SYNTHESIS OF RNA IN MAMMALIAN CELLS DURING MITOSIS AND INTERPHASE

1967 ◽  
Vol 33 (2) ◽  
pp. 265-272 ◽  
Author(s):  
Donald W. King ◽  
M. L. Barnhisel
Keyword(s):  
Tissue Culture ◽  
Nucleic Acid ◽  
Linear Relationship ◽  
Cell Population ◽  
Mammalian Cells ◽  
Short Pulse ◽  
Chinese Hamster ◽  
Growth Cycle ◽  
Chinese Hamster Cells ◽  
Number Of Cells

Chinese hamster cells in the mitotic and G1 phases of the growth cycle were incubated for 30 or 60 min in suspension tissue culture and pulse-labeled with tritiated uridine. After appropriate chases, washes, and extractions, it was found that all incorporation into the nucleic acid may be accounted for by those cells in interphase. An average of 410 counts was found for incorporation into the cell population (approximately 2.0 x 105 cells) of which over 80% of the cells was initially in mitosis. The increasing number of cells leaving mitosis and entering interphase during the 30 min incubation was theoretically able to account for 470 counts. In addition, short-pulse labeling experiments have shown a consistent linear relationship between the percentage of cells in division and the incorporation of the isotope, which strongly suggests that, if 100% of the cells were in mitosis, the counts would be essentially zero. Thus, the entire label may be attributed to those cells in interphase where portions of the chromosomal material are known to be already extended.

Cytotoxicity Study of Textile Fabrics Impregnated With CuO Nanoparticles in Mammalian Cells

2017 ◽  
Vol 36 (6) ◽  
pp. 478-484 ◽  
Author(s):  
Gagandeep Singh ◽  
James Beddow ◽  
Christopher Mee ◽  
Lidia Maryniak ◽  
Eadaoin M. Joyce ◽  
...  
Keyword(s):  
Cell Lines ◽  
Mammalian Cells ◽  
Dermal Fibroblast ◽  
Copper Oxide Nanoparticles ◽  
Growth Media ◽  
Indirect Contact ◽  
Cuo Nps ◽  
Mammalian Cell Lines ◽  
Textile Fabrics ◽  
Hdf Cells

Copper and copper compounds have multifunctional properties (antibacterial, antiviral, and antifungal) with promising applications. Copper in its nanoparticle (Cu NPs) forms has been widely used in various industrial and commercial applications. In the current research, the cytotoxic effects of textile fabrics impregnated with copper oxide nanoparticles (CuO NPs) were studied in mammalian cell lines. CuO NPs were impregnated onto textile substrates using 2 different techniques: the sonochemical generation and impregnation of NPs from metal complexes ( insitu) and a “throwing the stones” technology using commercially prepared CuO NPs. The cytotoxicity of these 2 textile fabric types was assayed on human dermal fibroblast (HDF) cells and human hepatocellular carcinoma cells (HepG2) and was evaluated by indirect contact using an MTT assay. The impregnated fabrics were not exposed to the cells, rather their leachates were used to test cytotoxicity. The fabrics were soaked into the growth media for up to 7 days, and the leachates from day 1 and day 7 were incubated with the cell lines for 24 hours prior to the testing. The discharge or leaching from antimicrobial nanomaterials into the surroundings and surface waters is posing a serious environmental threat, which needs to be addressed. Hence, with regard to product safety, it is a good approach to study the fabric leachates rather than the intact material. The results showed that CuO NPs are not toxic to HDF cells. However, cytotoxicity was seen in HepG2 cells with cell viability decreasing by 20% to 25% for all the fabrics after 24 hours.

scholarly journals Nucleic Acid Strand Displacement with Synthetic mRNA Inputs in Living Mammalian Cells

2018 ◽  
Vol 7 (12) ◽  
pp. 2737-2741 ◽  
Author(s):  
Gourab Chatterjee ◽  
Yuan-Jyue Chen ◽  
Georg Seelig
Keyword(s):  
Nucleic Acid ◽  
Mammalian Cells ◽  
Strand Displacement ◽  
Synthetic Mrna

scholarly journals 8-AZAGUANINE RESISTANCE IN MAMMALIAN CELLS I. HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE

Genetics ◽  
1972 ◽  
Vol 72 (2) ◽  
pp. 239-252 ◽  
Author(s):  
F D Gillin ◽  
D J Roufa ◽  
A L Beaudet ◽  
C T Caskey
Keyword(s):  
Nucleic Acid ◽  
Mammalian Cells ◽  
Chinese Hamster ◽  
Acid Synthesis ◽  
Ethyl Methanesulfonate ◽  
Nucleic Acid Synthesis ◽  
Wild Type ◽  
Chinese Hamster Cells ◽  
Hypoxanthine Guanine Phosphoribosyltransferase

ABSTRACT Chinese hamster cells were treated with ethyl methanesulfonate or N-methyl-N'-nitro-N-nitrosoguanidine, and mutants resistant to 8-azaguanine were selected and characterized. Hypoxanthine-guanine phosphoribosyltransferase activity of sixteen mutants is extremely negative, making them suitable for reversion to HGPRTase+. Ten of the extremely negative mutants revert at a frequency higher than 10-7 suggesting their point mutational character. The remaining mutants have demonstrable HGPRTase activity and are not useful for reversion analysis. Five of these mutants have < 2% HGPRTase and are presumably also HGPRTase point mutants. The remaining 14 mutants utilize exogenous hypoxanthine for nucleic acid synthesis poorly, and possess 20-150% of wild-type HGPRTase activity in in vitro. Their mechanism of 8-azaguanine resistance is not yet defined.

Nucleic Acid Electrotransfer in Mammalian Cells: Mechanistic Description

2017 ◽  
pp. 323-336 ◽  
Author(s):  
Muriel Golzio ◽  
Marie-Pierre Rols
Keyword(s):  
Nucleic Acid ◽  
Mammalian Cells
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