Functional Analysis of ESCRT-Positive Extracellular Vesicles in the Drosophila Wing Imaginal Disc

2019 ◽  
pp. 31-47
Author(s):  
Tamás Matusek ◽  
Pascal Thérond ◽  
Maximilian Fürthauer
Keyword(s):  
Functional Analysis ◽  
Extracellular Vesicles ◽  
Imaginal Disc ◽  
Wing Imaginal Disc ◽  
Drosophila Wing

scholarly journals Microvilli-derived extracellular vesicles carry Hedgehog morphogenic signals for Drosophila wing imaginal disc development

2021 ◽  
Author(s):  
Ilse Hurbain ◽  
Anne-Sophie Macé ◽  
Maryse Romao ◽  
Elodie Prince ◽  
Lucie Sengmanivong ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Imaginal Disc ◽  
Wing Imaginal Disc ◽  
Drosophila Wing

scholarly journals Compartments and the control of growth in the Drosophila wing imaginal disc

Development ◽  
2006 ◽  
Vol 133 (22) ◽  
pp. 4421-4426 ◽  
Author(s):  
F. A. Martin ◽  
G. Morata
Keyword(s):  
Imaginal Disc ◽  
Wing Imaginal Disc ◽  
Drosophila Wing

Control of growth and patterning of the Drosophila wing imaginal disc by EGFR-mediated signaling

Development ◽  
2002 ◽  
Vol 129 (6) ◽  
pp. 1369-1376 ◽  
Author(s):  
Myriam Zecca ◽  
Gary Struhl
Keyword(s):  
Gene Expression ◽  
Imaginal Disc ◽  
Ectopic Expression ◽  
Growth Factor Receptor ◽  
Wing Disc ◽  
Wing Imaginal Disc ◽  
Egfr Signaling ◽  
Drosophila Wing ◽  
Compartment Boundary ◽  
Egfr Ligand

The subdivision of the Drosophila wing imaginal disc into dorsoventral (DV) compartments and limb-body wall (wing-notum) primordia depends on Epidermal Growth Factor Receptor (EGFR) signaling, which heritably activates apterous (ap) in D compartment cells and maintains Iroquois Complex (Iro-C) gene expression in prospective notum cells. We examine the source, identity and mode of action of the EGFR ligand(s) that specify these subdivisions. Of the three known ligands for the Drosophila EGFR, only Vein (Vn), but not Spitz or Gurken, is required for wing disc development. We show that Vn activity is required specifically in the dorsoproximal region of the wing disc for ap and Iro-C gene expression. However, ectopic expression of Vn in other locations does not reorganize ap or Iro-C gene expression. Hence, Vn appears to play a permissive rather than an instructive role in organizing the DV and wing-notum segregations, implying the existance of other localized factors that control where Vn-EGFR signaling is effective. After ap is heritably activated, the level of EGFR activity declines in D compartment cells as they proliferate and move ventrally, away from the source of the instructive ligand. We present evidence that this reduction is necessary for D and V compartment cells to interact along the compartment boundary to induce signals, like Wingless (Wg), which organize the subsequent growth and differentiation of the wing primordium.

scholarly journals RNAi-Mediated Knockdown Showing Impaired Cell Survival in Drosophila Wing Imaginal Disc

2009 ◽  
Vol 3 ◽  
pp. GRSB.S2100 ◽  
Author(s):  
Makoto Umemori ◽  
Okiko Habara ◽  
Tatsunori Iwata ◽  
Kousuke Maeda ◽  
Kana Nishinoue ◽  
...  
Keyword(s):  
Cell Survival ◽  
Imaginal Disc ◽  
Wing Imaginal Disc ◽  
Drosophila Wing

Cell-level 3D reconstruction and quantification of the Drosophila wing imaginal disc

2019 ◽  
Vol 15 (2) ◽  
pp. 174
Author(s):  
Christian Dahmann ◽  
Frank Jülicher ◽  
Linge Bai ◽  
David E. Breen ◽  
Liyuan Sui
Keyword(s):  
3D Reconstruction ◽  
Imaginal Disc ◽  
Wing Imaginal Disc ◽  
Cell Level ◽  
Drosophila Wing

Boundaries in the Drosophila wing imaginal disc organize vein-specific genetic programs

Development ◽  
1998 ◽  
Vol 125 (21) ◽  
pp. 4245-4257 ◽  
Author(s):  
B. Biehs ◽  
M.A. Sturtevant ◽  
E. Bier
Keyword(s):  
Gene Expression ◽  
Cell Fate ◽  
Short Range ◽  
Imaginal Disc ◽  
Larval Instar ◽  
Wing Imaginal Disc ◽  
Control Of Gene Expression ◽  
Present Evidence ◽  
The Third ◽  
Drosophila Wing

Previous studies have suggested that vein primordia in Drosophila form at boundaries along the A/P axis between discrete sectors of the larval wing imaginal disc. Genes involved in initiating vein development during the third larval instar are expressed either in narrow stripes corresponding to vein primordia or in broader ‘provein’ domains consisting of cells competent to become veins. In addition, genes specifying the alternative intervein cell fate are expressed in complementary intervein regions. The regulatory relationships between genes expressed in narrow vein primordia, in broad provein stripes and in interveins remains unknown, however. In this manuscript, we provide additional evidence for veins forming in narrow stripes at borders of A/P sectors. These experiments further suggest that narrow vein primordia produce secondary short-range signal(s), which activate expression of provein genes in a broad pattern in neighboring cells. We also show that crossregulatory interactions among genes expressed in veins, proveins and interveins contribute to establishing the vein-versus-intervein pattern, and that control of gene expression in vein and intervein regions must be considered on a stripe-by-stripe basis. Finally, we present evidence for a second set of vein-inducing boundaries lying between veins, which we refer to as paravein boundaries. We propose that veins develop at both vein and paravein boundaries in more ‘primitive’ insects, which have up to twice the number of veins present in Drosophila. We present a model in which different A/P boundaries organize vein-specific genetic programs to govern the development of individual veins.

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