FTIR Analysis of Proteins and Protein–Membrane Interactions

2019 ◽  
pp. 281-325 ◽  
Author(s):  
Suren A. Tatulian
Keyword(s):  
Ftir Analysis ◽  
Membrane Interactions ◽  
Protein Membrane

Specific protein-membrane interactions promote packaging of metallo-β-lactamases into outer membrane vesicles

2021 ◽  
Author(s):  
Carolina López ◽  
Alessio Prunotto ◽  
Guillermo Bahr ◽  
Robert A. Bonomo ◽  
Lisandro J. González ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Electrostatic Interactions ◽  
Active Form ◽  
Membrane Vesicles ◽  
Specific Protein ◽  
Outer Membrane Vesicles ◽  
Soluble Enzyme ◽  
Membrane Interactions ◽  
Resistance Determinants ◽  
Protein Membrane

Outer membrane vesicles (OMVs) act as carriers of bacterial products such as plasmids and resistance determinants, including metallo-β-lactamases. The lipidated, membrane-anchored metallo-β-lactamase NDM-1 can be detected in Gram-negative OMVs. The soluble domain of NDM-1 also forms electrostatic interactions with the membrane. Herein, we show that these interactions promote its packaging into OMVs produced by Escherichia coli . We report that favorable electrostatic protein-membrane interactions are also at work in the soluble enzyme IMP-1, while being absent in VIM-2. These interactions correlate with an enhanced incorporation of IMP-1 compared to VIM-2 into OMVs. Disruption of these interactions in NDM-1 and IMP-1 impairs their inclusion into vesicles, confirming their role in defining the protein cargo in OMVs. These results also indicate that packaging of metallo-β-lactamases into vesicles in their active form is a common phenomenon that involves cargo selection based on specific molecular interactions.

scholarly journals Environmentally sensitive probes for monitoring protein-membrane interactions at nanomolar concentrations

2017 ◽  
Vol 1859 (5) ◽  
pp. 852-859 ◽  
Author(s):  
Volodymyr V. Shvadchak ◽  
Oleksandr Kucherak ◽  
Kseniia Afitska ◽  
Dmytro Dziuba ◽  
Dmytro A. Yushchenko
Keyword(s):  
Membrane Interactions ◽  
Protein Membrane ◽  
Environmentally Sensitive

scholarly journals Nanoscale studies of protein-membrane interactions in blood clotting

2011 ◽  
Vol 9 ◽  
pp. 162-167 ◽  
Author(s):  
J. H. MORRISSEY ◽  
E. TAJKHORSHID ◽  
C. M. RIENSTRA
Keyword(s):  
Blood Clotting ◽  
Membrane Interactions ◽  
Protein Membrane
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