Live-Cell Imaging of Fluorescently Tagged Phloem Proteins with Confocal Microscopy

2019 ◽  
pp. 95-108 ◽  
Author(s):  
Thibaud Cayla ◽  
Rozenn Le Hir ◽  
Sylvie Dinant
Keyword(s):  
Confocal Microscopy ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

Continuous live cell imaging of cellulose attachment by microbes under anaerobic and thermophilic conditions using confocal microscopy

2013 ◽  
Vol 25 (5) ◽  
pp. 849-856 ◽  
Author(s):  
Zhi-Wu Wang ◽  
Seung-Hwan Lee ◽  
James G. Elkins ◽  
Yongchao Li ◽  
Scott Hamilton-Brehm ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Thermophilic Conditions

scholarly journals KV1.5–KVβ1.3 Recycling Is PKC-Dependent

2021 ◽  
Vol 22 (3) ◽  
pp. 1336
Author(s):  
Alvaro Macias ◽  
Alicia de la Cruz ◽  
Diego A. Peraza ◽  
Angela de Benito-Bueno ◽  
Teresa Gonzalez ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Hek293 Cells ◽  
Dependent Manner ◽  
Fast Inactivation ◽  
Patch Clamp Technique ◽  
Relevant Issue ◽  
Regulatory Subunits

KV1.5 channel function is modified by different regulatory subunits. KVβ1.3 subunits assemble with KV1.5 channels and induce a fast and incomplete inactivation. Inhibition of PKC abolishes the KVβ1.3-induced fast inactivation, decreases the amplitude of the current KV1.5–KVβ1.3 and modifies their pharmacology likely due to changes in the traffic of KV1.5–KVβ1.3 channels in a PKC-dependent manner. In order to analyze this hypothesis, HEK293 cells were transfected with KV1.5–KVβ1.3 channels, and currents were recorded by whole-cell configuration of the patch-clamp technique. The presence of KV1.5 in the membrane was analyzed by biotinylation techniques, live cell imaging and confocal microscopy approaches. PKC inhibition resulted in a decrease of 33 ± 7% of channels in the cell surface due to reduced recycling to the plasma membrane, as was confirmed by confocal microscopy. Live cell imaging indicated that PKC inhibition almost abolished the recycling of the KV1.5–KVβ1.3 channels, generating an accumulation of channels into the cytoplasm. All these results suggest that the trafficking regulation of KV1.5–KVβ1.3 channels is dependent on phosphorylation by PKC and, therefore, they could represent a clinically relevant issue, mainly in those diseases that exhibit modifications in PKC activity.

Live Cell Imaging and Confocal Microscopy

2018 ◽  
pp. 117-130 ◽  
Author(s):  
Luciana Renna ◽  
Giovanni Stefano ◽  
Federica Brandizzi
Keyword(s):  
Confocal Microscopy ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell
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