Imaging rRNA Methylation in Bacteria by MR-FISH

2019 ◽  
pp. 89-107
Author(s):  
Kristina A. Ganzinger ◽  
Martin R. Challand ◽  
James Spencer ◽  
David Klenerman ◽  
Rohan T. Ranasinghe
Keyword(s):  
Rrna Methylation

scholarly journals Bypassing rRNA methylation by RsmA/Dim1during ribosome maturation in the hyperthermophilic archaeonNanoarchaeum equitans

2016 ◽  
pp. gkw839 ◽  
Author(s):  
Kenneth H. Seistrup ◽  
Simon Rose ◽  
Ulf Birkedal ◽  
Henrik Nielsen ◽  
Harald Huber ◽  
...  
Keyword(s):  
Rrna Methylation

scholarly journals AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana

2011 ◽  
Vol 65 (5) ◽  
pp. 807-819 ◽  
Author(s):  
Julie Rodor ◽  
Edouard Jobet ◽  
Jonathan Bizarro ◽  
Florence Vignols ◽  
Cristel Carles ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Development ◽  
Snorna Gene ◽  
Essential Protein ◽  
Gene Organisation ◽  
The Impact ◽  
Rrna Methylation

Temperature-sensitive mutations demonstrate roles for yeast fibrillarin in pre-rRNA processing, pre-rRNA methylation, and ribosome assembly

Cell ◽  
1993 ◽  
Vol 72 (3) ◽  
pp. 443-457 ◽  
Author(s):  
David Tollervey ◽  
Hanna Lehtonen ◽  
Ralf Jansen ◽  
Hildegard Kern ◽  
Eduard C. Hurt
Keyword(s):  
Ribosome Assembly ◽  
Temperature Sensitive ◽  
Rrna Processing ◽  
Rrna Methylation

rRNA Methylation and Antibiotic Resistance

2020 ◽  
Vol 85 (11) ◽  
pp. 1335-1349 ◽  
Author(s):  
I. A. Osterman ◽  
O. A. Dontsova ◽  
P. V. Sergiev
Keyword(s):  
Antibiotic Resistance ◽  
Rrna Methylation

Eukaryotic rRNA methylation: the calm before the Sno storm

1998 ◽  
Vol 23 (11) ◽  
pp. 447-450 ◽  
Author(s):  
B. Edward ◽  
H. Maden
Keyword(s):  
Rrna Methylation

scholarly journals 50S subunit recognition and modification by the Mycobacterium tuberculosis ribosomal RNA methyltransferase TlyA

2021 ◽  
Author(s):  
Zane T. Laughlin ◽  
Debayan Dey ◽  
Natalia Zelinskaya ◽  
Marta A. Witek ◽  
Pooja Srinivas ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Mycobacterium Tuberculosis ◽  
Ribosomal Rna ◽  
Substrate Recognition ◽  
Methylation Status ◽  
Drug Binding ◽  
Site Directed Mutagenesis ◽  
23S Rrna ◽  
Structural Reorganization ◽  
Rrna Methylation

Changes in bacterial ribosomal RNA (rRNA) methylation status can alter the activity of diverse groups of ribosome-targeting antibiotics. Typically, such modifications are incorporated by a single methyltransferase that acts on one nucleotide target and rRNA methylation directly prevents drug binding, thereby conferring drug resistance. However, loss of intrinsic methylation can also result in antibiotic resistance. For example, Mycobacterium tuberculosis (Mtb) becomes sensitized to tuberactinomycin antibiotics, such as capreomycin and viomycin, due to the action of the intrinsic methyltransferase TlyA. TlyA is unique among antibiotic resistance-associated methyltransferases as it has dual 16S and 23S rRNA substrate specificity and can incorporate cytidine-2'-O-methylations within two structurally distinct contexts. How TlyA accomplishes this feat of dual-target molecular recognition is currently unknown. Here, we report the structure of the Mtb 50S-TlyA subunit complex trapped in a post-catalytic state with a S-adenosyl-L-methionine analog using single-particle cryogenic electron microscopy. This structure, together with complementary site-directed mutagenesis and methyltransferase functional analyses, reveals critical roles in 23S rRNA substrate recognition for conserved residues across an interaction surface that spans both TlyA domains. These interactions position the TlyA active site over the target nucleotide C2144 which is flipped from 23S Helix 69 in a process stabilized by stacking of TlyA residue Phe157 on the adjacent A2143. This work reveals critical aspects of substrate recognition by TlyA and suggests that base flipping is likely a common strategy among rRNA methyltransferase enzymes even in cases where the target site is accessible without such structural reorganization.

scholarly journals The METTL5-TRMT112 N6-methyladenosine methyltransferase complex regulates mRNA translation via 18S rRNA methylation

2022 ◽  
pp. 101590
Author(s):  
Caraline Sepich-Poore ◽  
Zhong Zheng ◽  
Emily Schmitt ◽  
Kailong Wen ◽  
Zijie Scott Zhang ◽  
...  
Keyword(s):  
18S Rrna ◽  
Mrna Translation ◽  
Rrna Methylation

scholarly journals Analysis of the rRNA methylation complex components in pediatric B-cell precursor acute lymphoblastic leukemia: A pilot study

2020 ◽  
Vol 29 (1) ◽  
pp. 107-113
Author(s):  
Marek Ussowicz ◽  
Virginie Marcel ◽  
Flora Nguyen Van Long ◽  
Bernarda Kazanowska ◽  
Jean-Jacques Diaz ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Pilot Study ◽  
B Cell ◽  
Lymphoblastic Leukemia ◽  
Cell Precursor ◽  
Complex Components ◽  
Rrna Methylation
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