AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana

Abstract(+)-7-iso-Jasmonoyl-l-isoleucine (JA-Ile) is a lipid-derived phytohormone implicated in plant development, reproduction, and defense in response to pathogens and herbivorous insects. All these effects are instigated by the perception of JA-Ile by the COI1-JAZ co-receptor in the plant body, which in Arabidopsis thaliana is profoundly influenced by the short JAZ degron sequence (V/L)P(Q/I)AR(R/K) of the JAZ protein. Here, we report that SlJAZ-SlCOI1, the COI1-JAZ co-receptor found in the tomato plant, relies on the extended JAZ degron sequence (V/L)P(Q/I)AR(R/K)XSLX instead of the canonical JAZ degron. This finding illuminates our understanding of the mechanism of ligand perception by JA-Ile in this plant, and will inform both efforts to improve it by genetic modification of the SlCOI1-SlJAZ co-receptor, and the development of the synthetic agonists/antagonists.

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Systematic Phenotyping of Plant Development in Arabidopsis thaliana

Phenomics ◽

10.1201/b16437-7 ◽

2014 ◽

pp. 111-141

Author(s):

Christine Granier ◽

Vincent Nègre ◽

Fabio Fiorani

Keyword(s):

Arabidopsis Thaliana ◽

Plant Development

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Anti-Inflammatory Activity of A Polyphenolic Extract from Arabidopsis thaliana in In Vitro and In Vivo Models of Alzheimer’s Disease

International Journal of Molecular Sciences ◽

10.3390/ijms20030708 ◽

2019 ◽

Vol 20 (3) ◽

pp. 708 ◽

Cited By ~ 12

Author(s):

Roberto Mattioli ◽

Antonio Francioso ◽

Maria d’Erme ◽

Maurizio Trovato ◽

Patrizia Mancini ◽

...

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Arabidopsis Thaliana ◽

Heme Oxygenase 1 ◽

Neuroprotective Effects ◽

In Vivo Models ◽

Polyphenolic Extract ◽

Anti Inflammatory ◽

The Impact

Alzheimer’s disease (AD) is the most common neurodegenerative disorder and the primary form of dementia in the elderly. One of the main features of AD is the increase in amyloid-beta (Aβ) peptide production and aggregation, leading to oxidative stress, neuroinflammation and neurodegeneration. Polyphenols are well known for their antioxidant, anti-inflammatory and neuroprotective effects and have been proposed as possible therapeutic agents against AD. Here, we investigated the effects of a polyphenolic extract of Arabidopsis thaliana (a plant belonging to the Brassicaceae family) on inflammatory response induced by Aβ. BV2 murine microglia cells treated with both Aβ25–35 peptide and extract showed a lower pro-inflammatory (IL-6, IL-1β, TNF-α) and a higher anti-inflammatory (IL-4, IL-10, IL-13) cytokine production compared to cells treated with Aβ only. The activation of the Nrf2-antioxidant response element signaling pathway in treated cells resulted in the upregulation of heme oxygenase-1 mRNA and in an increase of NAD(P)H:quinone oxidoreductase 1 activity. To establish whether the extract is also effective against Aβ-induced neurotoxicity in vivo, we evaluated its effect on the impaired climbing ability of AD Drosophila flies expressing human Aβ1–42. Arabidopsis extract significantly restored the locomotor activity of these flies, thus confirming its neuroprotective effects also in vivo. These results point to a protective effect of the Arabidopsis extract in AD, and prompt its use as a model in studying the impact of complex mixtures derived from plant-based food on neurodegenerative diseases.

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The Translation Elongation Factor eEF-1Bβ1 Is Involved in Cell Wall Biosynthesis and Plant Development in Arabidopsis thaliana

PLoS ONE ◽

10.1371/journal.pone.0030425 ◽

2012 ◽

Vol 7 (1) ◽

pp. e30425 ◽

Cited By ~ 17

Author(s):

Zakir Hossain ◽

Lisa Amyot ◽

Brian McGarvey ◽

Margaret Gruber ◽

Jinwook Jung ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Cell Wall ◽

Plant Development ◽

Elongation Factor ◽

Cell Wall Biosynthesis ◽

Translation Elongation Factor ◽

Translation Elongation

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Arabidopsis thaliana SPF1 and SPF2 are nuclear-located ULP2-like SUMO proteases that act downstream of SIZ1 in plant development

Journal of Experimental Botany ◽

10.1093/jxb/ery265 ◽

2018 ◽

Vol 69 (19) ◽

pp. 4633-4649 ◽

Cited By ~ 12

Author(s):

Pedro Humberto Castro ◽

Miguel Ângelo Santos ◽

Sara Freitas ◽

Pepe Cana-Quijada ◽

Tiago Lourenço ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Plant Development ◽

Sumo Proteases

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The impact of light intensity on metabolomic profile of Arabidopsis thaliana wild type and reticulata mutant by NMR spectroscopy

Phytochemistry Letters ◽

10.1016/j.phytol.2018.06.013 ◽

2018 ◽

Vol 26 ◽

pp. 170-178 ◽

Cited By ~ 3

Author(s):

Tahereh Jafari ◽

Moona Rahikainen ◽

Elina Puljula ◽

Jari Sinkkonen ◽

Saijaliisa Kangasjärvi

Keyword(s):

Arabidopsis Thaliana ◽

Nmr Spectroscopy ◽

Light Intensity ◽

Metabolomic Profile ◽

Wild Type ◽

The Impact

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Engineered Ripening-Specific Accumulation of Polyamines Spermidine and Spermine in Tomato Fruit Upregulates Clustered C/D Box snoRNA Gene Transcripts in Concert with Ribosomal RNA Biogenesis in the Red Ripe Fruit

Plants ◽

10.3390/plants9121710 ◽

2020 ◽

Vol 9 (12) ◽

pp. 1710

Author(s):

Vijaya Shukla ◽

Tahira Fatima ◽

Ravinder K. Goyal ◽

Avtar K. Handa ◽

Autar K. Mattoo

Keyword(s):

Ribosomal Rna ◽

Tomato Fruit ◽

Rrna Genes ◽

Snorna Gene ◽

Ripe Fruit ◽

High Accumulation ◽

Specific Expression ◽

Significant Enrichment ◽

Transgenic Tomatoes ◽

The Impact

Ripening of tomato fruit leads, in general, to a sequential decrease in the endogenous levels of polyamines spermidine (SPD) and spermine (SPM), while the trend for the diamine putrescine (PUT) levels is generally an initial decrease, followed by a substantial increase, and thereafter reaching high levels at the red ripe fruit stage. However, genetic engineering fruit-specific expression of heterologous yeast S-adenosylmethionine (SAM) decarboxylase in tomato has been found to result in a high accumulation of SPD and SPM at the cost of PUT. This system enabled a genetic approach to determine the impact of increased endogenous levels of biogenic amines SPD and SPM in tomato (579HO transgenic line) and on the biogenesis, transcription, processing, and stability of ribosomal RNA (rRNA) genes in tomato fruit as compared with the non-transgenic 556AZ line. One major biogenetic process regulating transcription and processing of pre-mRNA complexes in the nucleus involves small nucleolar RNAs (snoRNAs). To determine the effect of high levels of SPD and SPM on these latter processes, we cloned, sequenced, and identified a box C/D snoRNA cluster in tomato, namely, SlSnoR12, SlU24a, Slz44a, and Slz132b. Similar to this snoRNA cluster housed on chromosome (Chr.) 6, two other noncoding C/D box genes, SlsnoR12.2 and SlU24b, with a 94% identity to those on Chr. 6 were found located on Chr. 3. We also found that other snoRNAs divisible into snoRNA subclusters A and B, separated by a uridine rich spacer, were decorated with other C/D box snoRNAs, namely, J10.3, Z131a/b, J10.1, and Z44a, followed by z132a, J11.3, z132b, U24, Z20, U24a, and J11. Several of these, for example, SlZ44a, Slz132b, and SlU24a share conserved sequences similar to those in Arabidopsis and rice. RNAseq analysis of high SPD/SPM transgenic tomatoes (579HO line) showed significant enrichment of RNA polymerases, ribosomal, and translational protein genes at the breaker+8 ripening stage as compared with the 556AZ control. Thus, these results indicate that SPD/SPM regulates snoRNA and rRNA expression directly or indirectly, in turn, affecting protein synthesis, metabolism, and other cellular activities in a positive manner.

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AtSec62 is critical for plant development and is involved in ER‐phagy in Arabidopsis thaliana

Journal of Integrative Plant Biology ◽

10.1111/jipb.12872 ◽

2019 ◽

Vol 62 (2) ◽

pp. 181-200 ◽

Cited By ~ 13

Author(s):

Shuai Hu ◽

Hao Ye ◽

Yong Cui ◽

Liwen Jiang

Keyword(s):

Arabidopsis Thaliana ◽

Plant Development

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The impact of epitranscriptomic marks on post-transcriptional regulation in plants

Briefings in Functional Genomics ◽

10.1093/bfgp/elaa021 ◽

2020 ◽

Author(s):

Xiang Yu ◽

Bishwas Sharma ◽

Brian D Gregory

Keyword(s):

Transcriptional Regulation ◽

Plant Development ◽

Messenger Rna ◽

Rna Modifications ◽

Abiotic And Biotic Stresses ◽

Rna Molecules ◽

Biotic Stresses ◽

Specific Mrnas ◽

Post Transcriptional Regulation ◽

The Impact

Abstract Ribonucleotides within the various RNA molecules in eukaryotes are marked with more than 160 distinct covalent chemical modifications. These modifications include those that occur internally in messenger RNA (mRNA) molecules such as N6-methyladenosine (m6A) and 5-methylcytosine (m5C), as well as those that occur at the ends of the modified RNAs like the non-canonical 5′ end nicotinamide adenine dinucleotide (NAD+) cap modification of specific mRNAs. Recent findings have revealed that covalent RNA modifications can impact the secondary structure, translatability, functionality, stability and degradation of the RNA molecules in which they are included. Many of these covalent RNA additions have also been found to be dynamically added and removed through writer and eraser complexes, respectively, providing a new layer of epitranscriptome-mediated post-transcriptional regulation that regulates RNA quality and quantity in eukaryotic transcriptomes. Thus, it is not surprising that the regulation of RNA fate mediated by these epitranscriptomic marks has been demonstrated to have widespread effects on plant development and the responses of these organisms to abiotic and biotic stresses. In this review, we highlight recent progress focused on the study of the dynamic nature of these epitranscriptome marks and their roles in post-transcriptional regulation during plant development and response to environmental cues, with an emphasis on the mRNA modifications of non-canonical 5′ end NAD+ capping, m6A and several other internal RNA modifications.

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The impact of temperature on blue light induced chloroplast movements in Arabidopsis thaliana

Plant Science ◽

10.1016/j.plantsci.2015.07.013 ◽

2015 ◽

Vol 239 ◽

pp. 238-249 ◽

Cited By ~ 18

Author(s):

Justyna Łabuz ◽

Paweł Hermanowicz ◽

Halina Gabryś

Keyword(s):

Arabidopsis Thaliana ◽

Blue Light ◽

Chloroplast Movements ◽

The Impact

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