scholarly journals Forward and Reverse Genetic Analysis of Chlamydia

2019 ◽  
pp. 185-204
Author(s):  
Mateusz Kędzior ◽  
Robert J. Bastidas
Keyword(s):  
Genetic Analysis ◽  
Reverse Genetic

scholarly journals Microinjection of mRNA or morpholinos for reverse genetic analysis in the starlet sea anemone, Nematostella vectensis

2013 ◽  
Vol 8 (5) ◽  
pp. 924-934 ◽  
Author(s):  
Michael J Layden ◽  
Eric Röttinger ◽  
Francis S Wolenski ◽  
Thomas D Gilmore ◽  
Mark Q Martindale
Keyword(s):  
Genetic Analysis ◽  
Sea Anemone ◽  
Nematostella Vectensis ◽  
Reverse Genetic

scholarly journals Reverse Genetic Analysis of the Transcription Regulatory Sequence of the Coronavirus Transmissible Gastroenteritis Virus

2004 ◽  
Vol 78 (11) ◽  
pp. 6061-6066 ◽  
Author(s):  
Kristopher M. Curtis ◽  
Boyd Yount ◽  
Amy C. Sims ◽  
Ralph S. Baric
Keyword(s):  
Genetic Analysis ◽  
Full Length ◽  
Transmissible Gastroenteritis Virus ◽  
Regulatory Sequence ◽  
Reverse Genetic ◽  
Conserved Sequence ◽  
Gastroenteritis Virus ◽  
Flanking Sequences ◽  
Transmissible Gastroenteritis ◽  
Discontinuous Transcription

ABSTRACT Coronavirus discontinuous transcription uses a highly conserved sequence (CS) in the joining of leader and body RNAs. Using a full-length infectious construct of transmissable gastroenteritis virus, the present study demonstrates that subgenomic transcription is heavily influenced by upstream flanking sequences and supports a mechanism of transcription attenuation that is regulated in part by a larger domain composed of primarily upstream flanking sequences which select appropriately positioned CS elements for synthesis of subgenomic RNAs.

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