Simultaneous Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Real-Time Multiplex qPCR Assay

2019 ◽  
pp. 27-32
Author(s):  
Kevin E. Eboigbodin
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Real Time ◽  
Simultaneous Detection ◽  
Qpcr Assay ◽  
Multiplex Qpcr

scholarly journals P2.015 Evaluation of the Triplex Real-Time PCR Assay For Detection of Chlamydia Trachomatis and Neisseria Gonorrhoeae in Urine and Vaginal Swabs

2013 ◽  
Vol 89 (Suppl 1) ◽  
pp. A92.1-A92
Author(s):  
T Samleerat ◽  
S Pookkapund ◽  
S Techanun ◽  
K Jitvatcharanon ◽  
P Leechanachai
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Vaginal Swabs

Development of multiplex real-time quantitative PCR for simultaneous detection of Chlamydia trachomatis and Ureaplasma parvum

2012 ◽  
Vol 45 (9) ◽  
pp. 663-667 ◽  
Author(s):  
Hong-bo Wei ◽  
She-xiao Zou ◽  
Xiao-lin Yang ◽  
Dai-qin Yang ◽  
Xiang-dong Chen
Keyword(s):  
Chlamydia Trachomatis ◽  
Real Time ◽  
Quantitative Pcr ◽  
Simultaneous Detection ◽  
Ureaplasma Parvum ◽  
Real Time Quantitative Pcr

Comparison of three real-time PCR assays for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in young pregnant women

2015 ◽  
Vol 83 (4) ◽  
pp. 335-337 ◽  
Author(s):  
Olivia Peuchant ◽  
Sabrina de Diego ◽  
Chloé Le Roy ◽  
Sandrine Frantz-Blancpain ◽  
Claude Hocké ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Pregnant Women ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assays

scholarly journals Multiplex qPCR Assay for Direct Detection and Quantification of Colletotrichum truncatum, Corynespora cassiicola, and Sclerotinia sclerotiorum in Soybean Seeds

Plant Disease ◽  
2020 ◽  
Vol 104 (11) ◽  
pp. 3002-3009
Author(s):  
Maísa Ciampi-Guillardi ◽  
Juliana Ramiro ◽  
Maria Heloisa Duarte de Moraes ◽  
Marina Coan Goldoni Barbieri ◽  
Nelson S. Massola
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Internal Control ◽  
Direct Detection ◽  
Simultaneous Detection ◽  
Plant Diseases ◽  
Qpcr Assay ◽  
Soybean Seeds ◽  
Corynespora Cassiicola ◽  
Colletotrichum Truncatum ◽  
Multiplex Qpcr

Precise diagnosis of plant diseases is one of the most effective tools to minimize yield losses. Colletotrichum truncatum, Corynespora cassiicola, and Sclerotinia sclerotiorum are common soilborne pathogens that affect soybeans all over the world. We developed a multiplex quantitative real-time polymerase chain reaction (qPCR) assay to simultaneously detect and quantify the three pathogens in soybean seeds and to survey their occurrence in the main soybean production areas in Brazil. Species-specific primers and probes for C. truncatum and C. cassiicola were designed based on GAPDH and TEF1 genes, respectively, to be combined with qPCR detection of S. sclerotiorum previously reported. The multiplex qPCR assay was successful in the simultaneous detection of C. truncatum, C. cassiicola, and S. sclerotiorum, along with a host internal control. The four pathogens were detected and quantified in artificially and naturally infested soybean seeds, even in the lowest incidence level tested of 0.0625% or 1 infected seed out of 1,599 healthy ones. From 81 seed samples tested, C. truncatum was the most frequently detected pathogen and with higher incidence levels (0.25 to 0.125%), followed by S. sclerotiorum and C. cassiicola, both with lower incidence levels (0.125 to 0.0625%). Together, the results evidenced the high sensitivity of the multiplex qPCR assay, indicating its usefulness for a quick and reliable diagnosis of soybean diseases in seeds.

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