A reverse transcription-cross-priming amplification method with lateral flow dipstick assay for the rapid detection of Bean pod mottle virus

Bean pod mottle virus (BPMV) is a destructive virus that causes serious economic losses in many countries every year, highlighting the importance of its effective detection. In this study, we developed a fast reverse transcription-cross-priming amplification (RT-CPA) coupled with lateral flow dipstick (LFD) diagnostic method for BPMV detection. The RT-CPA-LFD assay that targets the coat protein gene of BPMV was highly specific against diagnosing four other common viruses transmitted by soybean seeds, i.e., Southern bean mosaic virus (SBMV), Tomato ringspot virus (ToRSV), Arabis mosaic virus (ArMV), and Tobacco ringspot virus (TRSV). The sensitivities of the real-time fluorescent RT-CPA and the RT-CPA-LFD assay were at least 50 pg/μl and 500 pg/μl, respectively. Despite a compromise in the limit of detection of the RT-CPA method compared with TaqMan-MGB real-time RT-PCR, our results demonstrated a notably better performance in the detection of field samples of BPMV-infested soybean seeds. With the advantages of efficiency and convenience by visual determination, the RT-CPA-LFD assay presents a potential application for the rapid and accurate detection of BPMV in routine tests.

Functional Divergence of G and Its Homologous Genes for Green Pigmentation in Soybean Seeds

The degradation of chlorophyll in mature soybean seeds is closely related to the development of their yellow color. In this study, we examined G, its homologue G-like (GL), and their mutant alleles and investigated the relationship between these genes and chlorophyll accumulation in the seed coats of mature seeds. Transient expression of G and GL proteins fused with green fluorescent protein revealed that both were localized in plastids. Overexpression of G resulted in the accumulation of chlorophyll in the seed coats and cotyledons of mature seeds, indicating that high expression levels of G result in chlorophyll accumulation that exceeds its metabolism in the seeds of yellow soybean. Analysis of near isogenic lines at the G locus demonstrated a significant difference in the chlorophyll content of the seed coats and cotyledons of mature seeds when G and mutant g alleles were expressed in the d1d2 stay-green genetic background, indicating that the G protein might repress the SGR-independent degradation of chlorophyll. We examined the distribution of mutant alleles at the G and GL loci among cultivated and wild soybean germplasm. The g allele was widely distributed in cultivated soybean germplasm, except for green seed coat soybean lines, all of which contained the G allele. The gl alleles were much fewer in number than the g alleles and were mainly distributed in the genetic resources of cultivated soybean from Japan. None of the landraces and breeding lines investigated in this study were observed to contain both the g and gl alleles. Therefore, in conclusion, the mutation of the G locus alone is essential for establishing yellow soybeans, which are major current soybean breeding lines.

Karakteristik Organoleptik dari tempe modifikasi dengan kacang kedelai hitam (Glycine max (L) Merrit)

Tempeh is a fermented food from soybean seeds using the fungus Rhizopus oligosporus. Tempeh is usually made from ordinary fermented soybeans. However, there are various types of beans besides ordinary soybeans, one of which is black soybeans. Food product innovation is closely related to consumer acceptance. Acceptance can be measured using organoleptic test. Organoleptic testing is an important component in the process of making food product innovations. In this study, tempeh was made with a mixture of soybeans and black soybeans in a ratio of 50:50. The resulting tempeh was then tested organoleptically including the parameters of color, texture, aroma, and overall and compared with commercial tempeh on 45 panelists. Based on this research, it is known that the overall parameters, namely color, texture, aroma, and overall in soybean tempeh and 50:50 black soybeans have lower acceptability than commercial tempeh so that further development of these products is needed so that consumer acceptance is higher increase.

Results of laboratory and bench-scale studies of seed dispensers with an electric drive of working tools

Mechanical and pneumatic seed drills of both domestic and foreign production are used in Russian farms. They are equipped with a mechanical drive of working tools and an electronic seeding control system. Due to the slipping of the wheels or the breakage of the chains, the sowing of seeds in individual seed dispensers interrupts. According to the results of laboratory and bench-scale studies in respect to soybean seeds, the required power for the electric drive of one seed dispenser was determined, which, depending on the disk rotation speed from 10 to 60 rpm, ranged from 30 to 120 W. By calculation, using the analytical expression, the power, required for the fan drive of a 12-row seed drill, was determined, which, depending on the disk rotation speed, ranged from 1.6 to 2.47 kW. A condition is formulated, which will eliminate the probability of shifting and rolling seeds along the furrow after their fall out of the sowing disc rotating in the opposite direction to the movement of the seeder unit, provided correspondence of the linear speed of the sowing disc and the speed of the seeder unit (the effect of zero overlaps). In this case, the trajectory length of the seeds falling to the furrow should be consistent with the speed of the seeder unit and the seeding rate according to the proposed expression.

Genome-Wide Association Study Identifies Candidate Genes Related to the Linoleic Acid Content in Soybean Seeds

Soybean (Glycine max (L.) Merrill) oil is a complex mixture of five fatty acids (palmitic, stearic, oleic, linoleic, and linolenic). The high content of linoleic acid (LA) contributes to the oil having poor oxidative stability. Therefore, soybean seed with a lower LA content is desirable. To investigate the genetic architecture of LA, we performed a genome-wide association study (GWAS) using 510 soybean cultivars collected from China. The phenotypic identification results showed that the content of LA varied from 36.22% to 72.18%. The GWAS analysis showed that there were 37 genes related to oleic acid content, with a contribution rate of 7%. The candidate gene Glyma.04G116500.1 (GmWRI14) on chromosome 4 was detected in three consecutive years. The GmWRI14 showed a negative correlation with the LA content and the correlation coefficient was −0.912. To test whether GmWRI14 can lead to a lower LA content in soybean, we introduced GmWRI14 into the soybean genome. Matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry (MALDI-TOF IMS) showed that the overexpression of GmWRI14 leads to a lower LA content in soybean seeds. Meanwhile, RNA-seq verified that GmWRI14-overexpressed soybean lines showed a lower accumulation of GmFAD2-1A and GmFAD2-1B than control lines. Our results indicate that the down-regulation of the FAD2 gene triggered by the transcription factor GmWRI14 is the underlying mechanism reducing the LA level of seed. Our results provide novel insights into the genetic architecture of LA and pinpoint potential candidate genes for further in-depth studies.

Evaluation of the herbicide efficiency on soybean crops

Purpose. To study the effectiveness of soybean weed control systems. Methods. Field and laboratory. Results. It was investigated that the use of Pari herbicide in addition to the impact on weed plants as a result of their direct contact with the product also has a noticeable soil effect, and therefore treatment with a rate of 1.0 l/ha was effective in controlling 88.4% of seedlings weeds. The product showed high selectivity in terms of destruction of both dicotyledonous and monocotyledonous species presented in the experimental site. However, the application of Pari twice with a consumption rate of 0.3 l/ha and on the fourth day + 0.4 l/ha proved to be less effective and 79.0% of weeds died under this scheme. This is most likely due to the difficulty of applying small amounts of the product, in addition, re-application required a second pass of the sprayer, which means that part of the lines lost the protective soil film of the herbicide. We determined the best parameters of plant height in the control treatment, but the indicators of weed control, regardless of the phase of the experiment, were the worst. In addition, in the case of herbicide applications, we obtained values close to the control with slight differences in plant height, which may be due to experimental error. Conclusions. It was determined that the herbicide Pulsar 40 proved to be the most effective in the experiment, so with the application of 0.3 l/ha and on the fourth day + 0.4 l/ha, 86.9% of weeds common in soybean crops died. Among herbicides with a combined seed and soil action, Pari and Fabian were effective in applying them once at a full rate. Fabian herbicide was milder than cultivated plants due to the combined active ingredients and lower dose of imazethapyr compared to Pari herbicide. Therefore, the application of 0.1 kg/ha of Fabian yields 3.11 t/ha of soybean seeds. With the use of exclusively germinating preparations, the application of Pulsar 40 at a dose of 0.3 l/ha on the fourth day + 0.4 l/ha was effective, which allowed to form 3.08 t/ha of soy quenching.

Anatomical features of conductive tissues of soybeans that prevent the penetration of pathogenic agents of bacterial blight into seeds

The ways of penetration of pathogenic bacteria from the infected vegetative parts of plants into soybean seeds remain practically unexplored. It is widely believed that soybean seeds are infected through the vascular system from already infected areas of the vegetative parts. The aim of the present research was to study the possibility of penetration of pathogens of bacterial blight into soybean seeds through the conductive tissues of plants. The studies were carried out in 2019–2021 in V.S. Pustovoit All-Russian Research Institute of Oil Crops on plants and seeds of soybean variety Vilana. It was found that the size of stomatal slots in soybean leaves is 8–12 µm. This ensures free penetration of bacteria with a diameter of 1.3–1.7 µm into the leaf mesophyll. The pore sizes of the sieve plates of the phloem range from 0.4–0.7 to 0.8–1.6 µm, depending on the age of the plants. The largest pores of the phloem sieve plates are comparable to the diameters of pathogenic bacteria. However, a large number of transverse sieve plates located in the vessels of the phloem every 0.05–0.1 mm will filter and partially retain bacteria in each sieve tube along the path of cell sap in the phloem. Therefore, the pathogenic bacteria passing through the entire phloem from leaves infected with bacteriosis up to pods is physically unlikely. In pods, the vascular system ends in the area of attachment of the placenta to the seed hilum. In the hilum, there are no conductive tissues, and the further flow of water and nutrients into the seed is carried out diffusely through the plasmodesma of cell walls. It was found that the anatomical structure of the soybean phloem prevents the free movement of pathogenic bacteria along the conductive system directly into the inner tissues of the seeds. Therefore, the hypothesis of infection of soybean seeds with pathogens of bacterial blight through the conducting system of the plant should be considered untenable

Results of studies on justification of design and technological parameters of the feeding and dosing device in the technology of producing analogues of whole milk

Currently in the Amur region there is a gradual increase in the production of livestock products. The main limiting factor in this area is feed production. It should be noted that in the northern regions of the region, small-scale production is widely used in the framework of peasant farms. This is due to the fact that the fields in these areas suitable for sowing, as a rule, have small areas and the use of energy-rich equipment for the purpose of crop production is unprofitable. In this connection, it is advisable to engage in animal husbandry in these areas. An important component of effective production is the availability of raw materials of the required quality at the optimum cost in combination with the use of productive technologies and technical means for processing them into products with the maximum degree of compliance with the physiological needs of farm animals and poultry. Studies have shown that feed mixtures meet the requirements of the physiology of animals and poultry most fully, and, in particular, composite feed mixtures containing both high-protein components and vitamin-mineral complexes of natural origin. Thus, studies aimed at solving the problem of obtaining feed products based on raw material compositions using such a high-protein raw material available in the Amur Region as soybean seeds are important and relevant for the agricultural production of the region. The presented article presents the results of research on the production of feed products based on the use of soybean seeds. Keywords: FODDER PRODUCTION, SOYBEANS, FEEDING, YOUNG FARM ANIMALS, PREPARATION PROCESS, FEEDING AND DISPENSING DEVICE, EFFICIENCY

Comparative Analysis and Development of a Flavor Fingerprint for Volatile Compounds of Vegetable Soybean Seeds Based on Headspace-Gas Chromatography-Ion Mobility Spectrometry

Evaluating the volatile compounds and characteristic fingerprints of the core cultivars of vegetable soybean would provide useful data for improving their aroma in the breeding programs. The present study used headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) to evaluate the volatile compounds of vegetable soybean seeds at a specific growth stage. In total, 93 signal peaks were identified, 63 compounds qualitatively, with 14 volatile flavor compounds providing multiple signals. The 63 volatile compounds consisted of 15 esters, 15 aldehydes, 13 alcohols, 15 ketones, one acid, and four other compounds. The peak intensity of most of the volatile compounds varied greatly between the core cultivars. The alcohols and aldehydes determined the basic volatile flavor of the vegetable soybean seeds. Volatile flavors were determined by their respective esters, ketones, or other components. Characteristic fingerprints were found in some core vegetable soybean cultivars. Four cultivars (Xiangdou, ZHE1754, Zhexian 65018-33, and Qvxian No. 1) had pleasant aromas, because of their higher content of 2-acetyl-1-pyrroline (2-AP). A principal component analysis (PCA) was used to distinguish the samples based on the signal intensity of their volatile components. The results showed that the composition and concentration of volatile compounds differed greatly between the core cultivars, with the volatile flavor compounds of soybeans being determined by the ecotype of the cultivar, the direction of breeding selection, and their geographical origin. Characteristic fingerprints of the cultivars were established by HS-GC-IMS, enabling them to be used to describe and distinguish cultivars and their offspring in future breeding studies.

The Interaction between Bradyrhizobium Japonicum E109 and Azospirillum Brasilense Az39 Improves Bradyrhizobium-Soybean Symbiosis: The Secrets Behind Co-Inoculation

Aims The aim of this work was evaluating the interaction between two of the most widely used strains for soybean inoculation in Argentina, B. japonicum E109 (BjE109) and A. brasilense Az39 (AbAz39). Methods Five treatments were performed: (i) uninoculated seeds; (ii) seeds inoculated with BjE109; (iii) seeds inoculated with AbAz39; (iv) seeds co-inoculated with BjE109 and AbAz39 in a 1:1 proportion (BjE109 + AbAz39) at the seeds sowing and (v) seeds inoculated with a 1:1 proportion of BjE109 and AbAz39 (BjE109-AbAz39) 24 h before seeds sowing. Each treatment was assessed through a seed recovery assay, glasshouse assays and field assays. Results The combination between the two strains improved the ability of BjE109 to survive on soybean seeds after inoculation partially explained by AbAz39’s capacity to produce diverse biologically active molecules. As a result of the greater rhizobial survival on seeds the nodulation values and symbiosis parameters like nodule number, size and biomass and nodulation percentage also increased. In agreement with these observations, combining BjE109 and AbAz39 at strains the grain yield under field conditions were 13,3 and 17,3% greater than single BjE109 inoculation. Conclusions These results here show that the pre-culture combining BjE109 and AbAz39 before the inoculation to the soybean seeds has benefits in plant nodulation and hence production, more than individual inoculation with BjE109 or AbAz39, or the immediate co-inoculation of both strains.