Development of multiplex real-time quantitative PCR for simultaneous detection of Chlamydia trachomatis and Ureaplasma parvum

2012 ◽  
Vol 45 (9) ◽  
pp. 663-667 ◽  
Author(s):  
Hong-bo Wei ◽  
She-xiao Zou ◽  
Xiao-lin Yang ◽  
Dai-qin Yang ◽  
Xiang-dong Chen
Keyword(s):  
Chlamydia Trachomatis ◽  
Real Time ◽  
Quantitative Pcr ◽  
Simultaneous Detection ◽  
Ureaplasma Parvum ◽  
Real Time Quantitative Pcr

scholarly journals Simultaneous Detection of Influenza Viruses A and B Using Real-Time Quantitative PCR

2001 ◽  
Vol 39 (1) ◽  
pp. 196-200 ◽  
Author(s):  
L. J. R. van Elden ◽  
M. Nijhuis ◽  
P. Schipper ◽  
R. Schuurman ◽  
A. M. van Loon
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Simultaneous Detection ◽  
Influenza Viruses ◽  
Real Time Quantitative Pcr

Simultaneous detection of Waddlia chondrophila and Listeria monocytogenes in aborted ruminant samples by real-time quantitative PCR

2016 ◽  
Vol 125 ◽  
pp. 64-69 ◽  
Author(s):  
Mohamed Barkallah ◽  
Yaakoub Gharbi ◽  
Ahlem Ben Slima ◽  
Fatma Elleuch ◽  
Zouhir Mallek ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Real Time ◽  
Quantitative Pcr ◽  
Simultaneous Detection ◽  
Real Time Quantitative Pcr

Quantification and Differentiation of Campylobacter jejuni and Campylobacter coli in Raw Chicken Meats Using a Real-Time PCR Method

2007 ◽  
Vol 70 (9) ◽  
pp. 2015-2022 ◽  
Author(s):  
JOONBAE HONG ◽  
WOO KYUNG JUNG ◽  
JUN MAN KIM ◽  
SO HYUN KIM ◽  
HYE CHEONG KOO ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
High Specificity ◽  
Campylobacter Coli ◽  
Pcr Assay ◽  
The Real ◽  
Real Time Quantitative Pcr ◽  
Campylobacter Species

Campylobacter species are one of the most common causes of bacterial diarrhea in humans worldwide. The consumption of foods contaminated with two Campylobacter species, C. jejuni and C. coli, is usually associated with most of the infections in humans. In this study, a rapid, reliable, and sensitive multiplex real-time quantitative PCR was developed for the simultaneous detection, identification, and quantification of C. jejuni and C. coli. In addition, the developed method was applied to the 50 samples of raw chicken meat collected from retail stores in Korea. C. jejuni and C. coli were detected in 88 and 86% of the samples by real-time quantitative PCR and the conventional microbiological method, respectively. The specificity of the primer and probe sets was confirmed with 30 C. jejuni, 20 C. coli, and 35 strains of other microbial species. C. jejuni and C. coli could be detected with high specificity in less than 4 h, with a detection limit of 1 log CFU/ml by the developed real-time PCR. The average counts (log CFU per milliliter) of C. jejuni or C. coli obtained by the conventional methods and by the real-time PCR assay were statistically correlated with a correlation coefficient (R2) between 0.73 and 0.78. The real-time PCR assay developed in this study is useful for screening for the presence and simultaneous differential quantification of C. jejuni and C. coli.

scholarly journals Quantification and Stability of Human Adenoviruses and Polyomavirus JCPyV in Wastewater Matrices

2006 ◽  
Vol 72 (12) ◽  
pp. 7894-7896 ◽  
Author(s):  
Silvia Bofill-Mas ◽  
Nestor Albinana-Gimenez ◽  
Pilar Clemente-Casares ◽  
Ayalkibet Hundesa ◽  
Jesus Rodriguez-Manzano ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
High Stability ◽  
Wastewater Sludge ◽  
Human Polyomavirus ◽  
Sewage Effluent ◽  
Viral Contamination ◽  
Human Adenoviruses ◽  
Urban Sewage ◽  
Real Time Quantitative Pcr

ABSTRACT Human adenoviruses (HAdV) and human polyomavirus JCPyV have been previously proposed as indicators of fecal viral contamination in the environment. Different wastewater matrices have been analyzed by applying real-time quantitative PCR procedures for the presence, quantity, and stability of a wide diversity of excreted HAdV and JCPyV. High quantities of HAdV and JCPyV were detected in sewage, effluent wastewater, sludge, and biosolid samples. Both viruses showed high stability in urban sewage. These results confirm the suitability of both viruses as indicators of human fecal viral pollution.

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