Clinical Analysis of Structural Hemoglobin Variants and Hb A1c, by Capillary Isoelectric Focusing

2001 ◽  
pp. 145-163
Author(s):  
James M. Hempe ◽  
Alfonso Vargas ◽  
Randall D. Craver
Keyword(s):  
Isoelectric Focusing ◽  
Clinical Analysis ◽  
Capillary Isoelectric Focusing ◽  
Hb A1c ◽  
Hemoglobin Variants

Capillary isoelectric focusing of hemoglobin variants in the pediatric clinical laboratory

1997 ◽  
Vol 18 (10) ◽  
pp. 1785-1795 ◽  
Author(s):  
James M. Hempe ◽  
Jerry N. Granger ◽  
Randall D. Craver
Keyword(s):  
Isoelectric Focusing ◽  
Clinical Laboratory ◽  
Capillary Isoelectric Focusing ◽  
Hemoglobin Variants

Quantification of hemoglobin variants by capillary isoelectric focusing

1994 ◽  
Vol 40 (12) ◽  
pp. 2288-2295 ◽  
Author(s):  
J M Hempe ◽  
R D Craver
Keyword(s):  
Los Angeles ◽  
Isoelectric Focusing ◽  
Sickle Cell Trait ◽  
Low Pressure ◽  
Clinical Analysis ◽  
Capillary Isoelectric Focusing ◽  
Automated Method ◽  
Hb S ◽  
Assay Precision ◽  
Automated Data Acquisition

Abstract Capillary isoelectric focusing (cIEF) was used to identify and quantify major and minor hemoglobin (Hb) variants. Whole blood (approximately 10 microL required) hemolysate was analyzed with a commercial instrument equipped with a 50 microns (i.d.) x 27 cm coated capillary filled with 20 g/L ampholytes (pH 6-8) in 4 g/L methylcellulose (MC). Cathode and anode solutions were 20 mol/L NaOH and 100 mol/L H3PO4 in MC, respectively. Samples (approximately 40 nL) were applied via autosampler by low-pressure injection, focused for 3 min at 30 kV, and mobilized by simultaneous voltage and low pressure past the detector, where absorbance at 415 nm was analyzed by an automated data acquisition system. Blood from subjects with sickle cell trait, Hb S/C disease, and various beta-thalassemias were analyzed by cIEF in < 15 min. cIEF was used to separate Hb S from Hb D-Los Angeles. Assay precision determined with commercial controls gave CV < 2% for Hb A and S, and 1-11% for minor Hb variants A2, F, and A1c. Results obtained by cIEF for patients' samples agreed well with values determined by conventional assays (r2 > 0.95). The results demonstrate that cIEF is a rapid, sensitive, high-resolution automated method for routine quantitative clinical analysis of Hb variants.

Application of dynamic capillary isoelectric focusing to the analysis of human hemoglobin variants

1994 ◽  
Vol 15 (1) ◽  
pp. 22-30 ◽  
Author(s):  
Sarah Molteni ◽  
Hannes Frischknecht ◽  
Wolfgang Thormann
Keyword(s):  
Isoelectric Focusing ◽  
Human Hemoglobin ◽  
Capillary Isoelectric Focusing ◽  
Hemoglobin Variants

scholarly journals Clinical Application of Capillary Isoelectric Focusing on Fused Silica Capillary for Determination of Hemoglobin Variants

1997 ◽  
Vol 43 (9) ◽  
pp. 1798-1799 ◽  
Author(s):  
Amin A Mohammad ◽  
Anthony O Okorodudu ◽  
Michael G Bissell ◽  
Pat Dow ◽  
Gregg Reger ◽  
...  
Keyword(s):  
Clinical Application ◽  
Isoelectric Focusing ◽  
Fused Silica ◽  
Capillary Isoelectric Focusing ◽  
Fused Silica Capillary ◽  
Hemoglobin Variants ◽  
Silica Capillary

scholarly journals Evaluation of the V8 E-Class, a Novel Automated Capillary Isoelectric Focusing Instrument for Hemoglobinopathy Screening

2016 ◽  
Vol 146 (3) ◽  
pp. 361-368 ◽  
Author(s):  
Toon Schiemsky ◽  
Caroline Brusselmans ◽  
Elke Nackers ◽  
Pieter Vermeersch ◽  
Koen Poesen ◽  
...  
Keyword(s):  
Isoelectric Focusing ◽  
Capillary Isoelectric Focusing ◽  
Class A
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