Creation and Use of a Cre Recombinase Transgenic Database

2009 ◽  
pp. 365-378 ◽  
Author(s):  
Andras Nagy ◽  
Lynn Mar ◽  
Graham Watts
Keyword(s):  
Cre Recombinase

scholarly journals Radiation-induced toxicity in rectal epithelial stem cell contributes to acute radiation injury in rectum

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Felipe Rodriguez Tirado ◽  
Payel Bhanja ◽  
Eduardo Castro-Nallar ◽  
Ximena Diaz Olea ◽  
Catalina Salamanca ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ex Vivo ◽  
Cre Recombinase ◽  
Lineage Tracing ◽  
Common Side Effect ◽  
Rectal Injury ◽  
Male Mice ◽  
Pelvic Irradiation ◽  
Pelvic Malignancies ◽  
Radiation Induced

Abstract Background Radiation-induced rectal epithelial damage is a very common side effect of pelvic radiotherapy and often compromise the life quality and treatment outcome in patients with pelvic malignancies. Unlike small bowel and colon, effect of radiation in rectal stem cells has not been explored extensively. Here we demonstrate that Lgr5-positive rectal stem cells are radiosensitive and organoid-based transplantation of rectal stem cells mitigates radiation damage in rectum. Methods C57Bl6 male mice (JAX) at 24 h were exposed to pelvic irradiation (PIR) to determine the radiation effect in pelvic epithelium. Effect of PIR on Lgr5-positive rectal stem cells (RSCs) was determined in Lgr5-EGFP-Cre-ERT2 mice exposed to PIR. Effect of PIR or clinically relevant fractionated PIR on regenerative response of Lgr5-positive RSCs was examined by lineage tracing assay using Lgr5-eGFP-IRES-CreERT2; Rosa26-CAG-tdTomato mice with tamoxifen administration to activate Cre recombinase and thereby marking the ISC and their respective progeny. Ex vivo three-dimensional organoid cultures were developed from Lgr5-EGFP-Cre-ERT2 mice. Organoid growth was determined by quantifying the budding crypt/total crypt ratio. Organoids from Lgr5-EGFP-ires-CreERT2-TdT mice were transplanted in C57Bl6 male mice exposed to PIR. Engraftment and repopulation of Lgr5-positive RSCs were determined after tamoxifen administration to activate Cre recombinase in recipient mice. Statistical analysis was performed using Log-rank (Mantel-Cox) test and paired two-tail t test. Result Exposure to pelvic irradiation significantly damaged rectal epithelium with the loss of Lgr5+ve rectal stem cells. Radiosensitivity of rectal epithelium was also observed with exposure to clinically relevant fractionated pelvic irradiation. Regenerative capacity of Lgr5+ve rectal stem cells was compromised in response to fractionated pelvic irradiation. Ex vivo organoid study demonstrated that Lgr5+ve rectal stem cells are sensitive to both single and fractionated radiation. Organoid-based transplantation of Lgr5+ve rectal stem cells promotes repair and regeneration of rectal epithelium. Conclusion Lgr5-positive rectal stem cells are radiosensitive and contribute to radiation-induced rectal epithelial toxicity. Transplantation of Lgr5-positive rectal stem cells mitigates radiation-induced rectal injury and promotes repair and regeneration process in rectum.

scholarly journals Characterization of Cre Recombinase Activity for In Vivo Targeting of Adipocyte Precursor Cells

2014 ◽  
Vol 3 (6) ◽  
pp. 1147-1158 ◽  
Author(s):  
Katherine C. Krueger ◽  
Maria José Costa ◽  
Hongqing Du ◽  
Brian J. Feldman
Keyword(s):  
Cre Recombinase ◽  
Precursor Cells ◽  
Adipocyte Precursor

Dilated Cardiomyopathy Resulting From High-Level Myocardial Expression of Cre-Recombinase

2006 ◽  
Vol 12 (5) ◽  
pp. 392-398 ◽  
Author(s):  
Antje Buerger ◽  
Olga Rozhitskaya ◽  
Megan C. Sherwood ◽  
Adam L. Dorfman ◽  
Egbert Bisping ◽  
...  
Keyword(s):  
Dilated Cardiomyopathy ◽  
Cre Recombinase ◽  
High Level

Transgenic mice that express Cre recombinase in osteoclasts

genesis ◽  
2004 ◽  
Vol 39 (3) ◽  
pp. 178-185 ◽  
Author(s):  
W.S.M. Chiu ◽  
J.F. McManus ◽  
A.J. Notini ◽  
A.I. Cassady ◽  
J.D. Zajac ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Cre Recombinase
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