Enriching Basic and Acidic Rat Brain Proteins with Ion Exchange Mini Spin Columns Before Two-Dimensional Gel Electrophoresis

2008 ◽  
pp. 157-166 ◽  
Author(s):  
Ning Liu ◽  
Aran Paulus
Keyword(s):  
Ion Exchange ◽  
Rat Brain ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Brain Proteins

Alterations in Rat Brain Proteins after Desflurane Anesthesia

2004 ◽  
Vol 100 (2) ◽  
pp. 302-308 ◽  
Author(s):  
Carsten D. Fütterer ◽  
Martin H. Maurer ◽  
Anne Schmitt ◽  
Robert E. Feldmann ◽  
Wolfgang Kuschinsky ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Expression ◽  
Rat Brain ◽  
Gel Electrophoresis ◽  
Volatile Anesthetics ◽  
Vesicle Transport ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Brain Proteins ◽  
The Brain

Background Volatile anesthetics disappear from an organism after the end of anesthesia. Whether changes of protein expression persist in the brain for a longer period is not known. This study investigates the question of whether the expression of proteins is altered in the rat brain after the end of desflurane anesthesia. Methods Three groups (n = 12 each) of rats were anesthetized with 5.7% desflurane in air for 3 h. Brains were removed directly after anesthesia, 24 h after anesthesia, or 72 h after anesthesia. Two additional groups (n = 12 each) served as naive conscious controls, in which the brains were removed without previous anesthesia 3 or 72 h after the start of the experiment. Cytosolic proteins were isolated. A proteome-wide study was performed, based on two-dimensional gel electrophoresis and mass spectrometry. Results Compared with conscious controls, significant (P < 0.05) increase/decrease was found: 3 h of anesthesia, 5/2 proteins; 24 h after anesthesia, 13/1 proteins; 72 h after anesthesia, 6/4 proteins. The overall changes in protein expression as quantified by the induction factor ranged from -1.67 (decrease to 60%) to 1.79 (increase by 79%) compared with the controls (100%). Some of these regulated proteins play a role in vesicle transport and metabolism. Conclusion Desflurane anesthesia produces changes in cytosolic protein expression up to 72 h after anesthesia in the rat brain, indicating yet unknown persisting effects.

Studies on catechol-O-methyltransferase in rat brain using two-dimensional gel electrophoresis

1986 ◽  
Vol 8 (4) ◽  
pp. 581-586 ◽  
Author(s):  
William E. Heydorn ◽  
G. Joseph Creed ◽  
Cyrus R. Creveling ◽  
David M. Jacobowitz
Keyword(s):  
Rat Brain ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

Identification of foetal brain proteins by two-dimensional gel electrophoresis and mass spectrometry

2000 ◽  
Vol 267 (15) ◽  
pp. 4713-4719 ◽  
Author(s):  
Madalina Oppermann ◽  
Neus Cols ◽  
Tuula Nyman ◽  
Jari Helin ◽  
Juhani Saarinen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Brain Proteins ◽  
Foetal Brain

Two-dimensional gel electrophoresis used in neurobiological studies of proteins in discrete areas of the rat brain.

1984 ◽  
Vol 30 (12) ◽  
pp. 1996-2002 ◽  
Author(s):  
D M Jacobowitz ◽  
W E Heydorn
Keyword(s):  
Animal Model ◽  
Rat Brain ◽  
Gel Electrophoresis ◽  
Chronic Administration ◽  
Cholinergic Innervation ◽  
Two Dimensional ◽  
Fresh Tissue ◽  
Two Dimensional Gel Electrophoresis ◽  
The Brain

Abstract Using two-dimensional gel electrophoresis, we studied proteins in the rat brain. The relative amounts of individual proteins differ in discrete areas of the brain, and the concentrations of three different proteins can be altered by chronic administration of desmethylimipramine or reserpine. Brain proteins can be radiolabeled in vitro by incubating samples of fresh tissue with [35S]methionine. We identified several proteins by using immunoblotting and comigration. Finally, we developed a possible animal model for studying proteins related to Alzheimer's disease by depleting the cholinergic innervation to the cortex and the hippocampus.

The whereabouts of transmembrane proteins from rat brain synaptosomes during two-dimensional gel electrophoresis

PROTEOMICS ◽  
2008 ◽  
Vol 8 (9) ◽  
pp. 1762-1770 ◽  
Author(s):  
Murat Eravci ◽  
Sandra Fuxius ◽  
Oliver Broedel ◽  
Stephanie Weist ◽  
Eberhard Krause ◽  
...  
Keyword(s):  
Rat Brain ◽  
Gel Electrophoresis ◽  
Transmembrane Proteins ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Rat Brain Synaptosomes ◽  
Brain Synaptosomes

Two-dimensional gel electrophoresis of human brain proteins. III. Genetic and non-genetic variations in 145 brains.

1982 ◽  
Vol 28 (4) ◽  
pp. 798-804 ◽  
Author(s):  
D E Comings
Keyword(s):  
Human Brain ◽  
Gel Electrophoresis ◽  
Low Frequency ◽  
Genetic Variations ◽  
Genetic Mutations ◽  
Two Dimensional ◽  
Chi Square ◽  
Two Dimensional Gel Electrophoresis ◽  
Brain Proteins ◽  
Chi Square Analysis

Abstract To determine the frequency of genetic mutations, polymorphisms, and non-genetic variation in the major human brain proteins, I examined, by equilibrium two-dimensional gel electrophoresis, 145 brains from patients dying of a wide variety of psychiatric, neurological, and non-neurological disorders. Of 176 polypeptides screened, there was one polymorphism of glial fibrillary acidic protein (GFAP-Duarte). Chi square analysis indicated it was non-randomly distributed among different diseases. A possible mutation associated with Joseph's disease is being further investigated. Three examples of a possible mutation of protein 8c:1 were noted. No other genetic mutations were observed. This low frequency of polymorphisms is consistent with results for two-dimensional gel analysis of other tissue and species. The numerous non-genetic variations are described.

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