Developmental Milestones in the Newborn Mouse

2008 ◽  
pp. 131-149 ◽  
Author(s):  
Joanna M. Hill ◽  
Maria A. Lim ◽  
Madeleine M. Stone
Keyword(s):  
Newborn Mouse ◽  
Developmental Milestones

Developmental milestones in early childhood and genetic liability to neurodevelopmental disorders

2020 ◽  
Author(s):  
Laurie John Hannigan ◽  
Ragna Bugge Askeland ◽  
Helga Ask ◽  
Martin Tesli ◽  
Elizabeth Corfield ◽  
...  
Keyword(s):  
Language Development ◽  
Motor Development ◽  
Neurodevelopmental Disorders ◽  
Autism Spectrum ◽  
Probit Regression ◽  
Developmental Milestones ◽  
Genetic Liability ◽  
Polygenic Scores ◽  
Walking Age ◽  
Children First

BackgroundEarly developmental milestones, such as the age at first walking or talking, are associated with later diagnoses of neurodevelopmental disorders, but the relationship to genetic risk for neurodevelopmental disorders are unknown. Here, we investigate associations between genetic liability to autism spectrum disorder (autism), attention deficit hyperactivity disorder (ADHD), and schizophrenia and attainment of early-life language and motor development milestones.MethodsWe use data from a genotyped sub-set (N = 15 205) of children in the Norwegian Mother, Father and Child Cohort Study (MoBa). In this sample, we calculate polygenic scores for autism; ADHD and schizophrenia and predict maternal reports of children’s age at first walking and talking, motor delays at 18 months, language delays at 3 years, and a generalized measure of concerns about development. We use linear and probit regression models in a multi-group framework to test for sex differences.ResultsADHD polygenic scores predicted earlier walking age in both males and females (β=-0.037, pFDR=0.001), and earlier first use of sentences (β=-0.087, pFDR=0.032) but delayed language development at 3 years in females only (β=0.194, pFDR=0.001). Additionally, we found evidence that autism polygenic scores were associated with later walking (β=0.027, pFDR=0.024) and motor delays at 18 months (β = 0.065, pFDR=0.028). Schizophrenia polygenic scores were associated with a measure of general concerns about development at 3 years in females only (β=0.132, pFDR=0.024).ConclusionsGenetic liabilities for neurodevelopmental disorders show some specific associations with measures of early motor and language development in the general population, including the age at which children first walk and talk. Associations are generally small and occasionally in unexpected directions. Sex differences are evident in some instances, but clear patterns across different polygenic scores and outcomes are hard to discern. These findings suggest that genetic susceptibility for neurodevelopmental disorders is manifested in the timing of developmental milestones in infancy.

scholarly journals Epidermal and hair follicle transglutaminases. Partial characterization of soluble enzymes in newborn mouse skin.

1988 ◽  
Vol 263 (9) ◽  
pp. 4236-4241 ◽  
Author(s):  
N Martinet ◽  
H C Kim ◽  
J E Girard ◽  
T P Nigra ◽  
D H Strong ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Mouse Skin ◽  
Partial Characterization ◽  
Newborn Mouse ◽  
Soluble Enzymes

Comparison of methods for teaching developmental milestones to pediatric residents

2011 ◽  
Vol 7 (2) ◽  
pp. 161-166 ◽  
Author(s):  
Marie Leiner ◽  
G. Prasad Krishnamurthy ◽  
O. Blanc ◽  
B. Castillo ◽  
I. Medina
Keyword(s):  
Comparison Of Methods ◽  
Developmental Milestones ◽  
Pediatric Residents

scholarly journals 1779 Cell Death Genes are Induced Immediately after Hypoxia-Reoxygenation (HR) in the Newborn Mouse Lung

2012 ◽  
Vol 97 (Suppl 2) ◽  
pp. A503-A503
Author(s):  
E. Wollen ◽  
A. Rognlien ◽  
M. Atneosen-Asegg ◽  
M. Wright ◽  
M. Bjoras ◽  
...  
Keyword(s):  
Cell Death ◽  
Mouse Lung ◽  
Newborn Mouse ◽  
Cell Death Genes ◽  
Death Genes ◽  
Hypoxia Reoxygenation

scholarly journals ABCA5 Resides in Lysosomes, and ABCA5 Knockout Mice Develop Lysosomal Disease-Like Symptoms

2005 ◽  
Vol 25 (10) ◽  
pp. 4138-4149 ◽  
Author(s):  
Yoshiyuki Kubo ◽  
Sayaka Sekiya ◽  
Megumi Ohigashi ◽  
Chiemi Takenaka ◽  
Kyoko Tamura ◽  
...  
Keyword(s):  
Thyroid Gland ◽  
Abc Transporter ◽  
Neural Cells ◽  
Newborn Mouse ◽  
Amino Acid Residues ◽  
Lysosomal Disease ◽  
Transmembrane Segments ◽  
Reverse Transcription Pcr ◽  
Localization Analysis ◽  
Immunohistochemical Studies

ABSTRACT ABCA5 is a member of the ABC transporter A subfamily, and a mouse orthologue (mABCA5) in newborn mouse brain and neural cells was identified by reverse transcription-PCR. Full-length cDNA cloning revealed that mABCA5 consists of 1,642 amino acid residues and that its putative structure is that of a full-type ABC transporter having two sets of six transmembrane segments and a nucleotide binding domain. Immunohistochemical studies revealed that mABCA5 is expressed in brain, lung, heart, and thyroid gland. A subcellular localization analysis showed that mABCA5 is a resident of lysosomes and late endosomes. Abca5 − / − mice exhibited symptoms similar to those of several lysosomal diseases in heart, although no prominent abnormalities were found in brain or lung. They developed a dilated cardiomyopathy-like heart after reaching adulthood and died due to depression of the cardiovascular system. In addition, Abca5 − / − mice also exhibited exophthalmos and collapse of the thyroid gland. Therefore, ABCA5 is a protein related to a lysosomal disease and plays important roles, especially in cardiomyocytes and follicular cells.

scholarly journals Construction of adenovirus vectors simultaneously expressing four multiplex, double-nicking guide RNAs of CRISPR/Cas9 and in vivo genome editing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tomoko Nakanishi ◽  
Aya Maekawa ◽  
Mariko Suzuki ◽  
Hirotaka Tabata ◽  
Kumiko Sato ◽  
...  
Keyword(s):  
Target Gene ◽  
Animal Experiments ◽  
Adenovirus Vector ◽  
Mouse Embryonic Fibroblast ◽  
Newborn Mouse ◽  
Guide Rnas ◽  
One Step ◽  
Target Effect

AbstractSimultaneous expression of multiplex guide RNAs (gRNAs) is valuable for knockout of multiple genes and also for effective disruption of a gene by introducing multiple deletions. We developed a method of Tetraplex-guide Tandem for construction of cosmids containing four and eight multiplex gRNA-expressing units in one step utilizing lambda in vitro packaging. Using this method, we produced an adenovirus vector (AdV) containing four multiplex-gRNA units for two double-nicking sets. Unexpectedly, the AdV could stably be amplified to the scale sufficient for animal experiments with no detectable lack of the multiplex units. When the AdV containing gRNAs targeting the H2-Aa gene and an AdV expressing Cas9 nickase were mixed and doubly infected to mouse embryonic fibroblast cells, deletions were observed in more than 80% of the target gene even using double-nicking strategy. Indels were also detected in about 20% of the target gene at two sites in newborn mouse liver cells by intravenous injection. Interestingly, when one double-nicking site was disrupted, the other was simultaneously disrupted, implying that two genes in the same cell may simultaneously be disrupted in the AdV system. The AdVs expressing four multiplex gRNAs could offer simultaneous knockout of four genes or two genes by double-nicking cleavages with low off-target effect.

scholarly journals Patterning of frontal cortex subdivisions by Fgf17

2007 ◽  
Vol 104 (18) ◽  
pp. 7652-7657 ◽  
Author(s):  
Jeremy A. Cholfin ◽  
John L. R. Rubenstein
Keyword(s):  
Gene Expression ◽  
Frontal Cortex ◽  
Newborn Mouse ◽  
Cortical Areas ◽  
Higher Cognition ◽  
Genetic Mechanisms

The frontal cortex (FC) is the seat of higher cognition. The genetic mechanisms that control formation of the functionally distinct subdivisions of the FC are unknown. Using a set of gene expression markers that distinguish subdivisions of the newborn mouse FC, we show that loss of Fgf17 selectively reduces the size of the dorsal FC whereas ventral/orbital FC appears normal. These changes are complemented by a rostral shift of sensory cortical areas. Thus, Fgf17 functions similar to Fgf8 in patterning the overall neocortical map but has a more selective role in regulating the properties of the dorsal but not ventral FC.

Occupational Therapy in Israel: An Overview of Developmental Milestones

2021 ◽  
Vol 4 (4) ◽  
Author(s):  
Liat Hen-Herbst ◽  
Tovi Margaliot ◽  
Eynat Gal ◽  
Orly Bouni ◽  
Sonya Meyer ◽  
...  
Keyword(s):  
Occupational Therapy ◽  
Developmental Milestones
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