Chemical Cleavage of Proteins at Methionyl Residues

1996 ◽  
pp. 369-373 ◽  
Author(s):  
Bryan John Smith
Keyword(s):  
Chemical Cleavage

Analysis of DNA sequences using a single chemical cleavage procedure

Biochemistry ◽  
1985 ◽  
Vol 24 (22) ◽  
pp. 6194-6200 ◽  
Author(s):  
Barbara J. B. Ambrose ◽  
Reynaldo C. Pless
Keyword(s):  
Dna Sequences ◽  
Chemical Cleavage ◽  
Single Chemical

scholarly journals Novel reagents for chemical cleavage at abasic sites and UV photoproducts in DNA

1995 ◽  
Vol 23 (10) ◽  
pp. 1664-1670 ◽  
Author(s):  
P. J. McHugh ◽  
J. Knowland
Keyword(s):  
Chemical Cleavage ◽  
Abasic Sites ◽  
Uv Photoproducts

Chemical Cleavage of Proteins at Asparaginyl-Glycyl Peptide Bonds

2003 ◽  
pp. 507-510 ◽  
Author(s):  
Bryan John Smith
Keyword(s):  
Peptide Bonds ◽  
Chemical Cleavage

scholarly journals Comparison of Strategies for the Determination of Sterol Sulfates via GC-MS Leading to a Novel Deconjugation-Derivatization Protocol

Molecules ◽  
2019 ◽  
Vol 24 (13) ◽  
pp. 2353 ◽  
Author(s):  
Julia Junker ◽  
Isabelle Chong ◽  
Frits Kamp ◽  
Harald Steiner ◽  
Martin Giera ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography Mass Spectrometry ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chemical Cleavage ◽  
Advantages And Disadvantages ◽  
Chromatography Mass Spectrometry ◽  
Steroid Profiling ◽  
Health And Disease ◽  
Indispensable Tool

Sulfoconjugates of sterols play important roles as neurosteroids, neurotransmitters, and ion channel ligands in health and disease. In most cases, sterol conjugate analysis is performed with liquid chromatography-mass spectrometry. This is a valuable tool for routine analytics with the advantage of direct sterol sulfates analysis without previous cleavage and/or derivatization. The complementary technique gas chromatography-mass spectrometry (GC-MS) is a preeminent discovery tool in the field of sterolomics, but the analysis of sterol sulfates is hampered by mandatory deconjugation and derivatization. Despite the difficulties in sample workup, GC-MS is an indispensable tool for untargeted analysis and steroid profiling. There are no general sample preparation protocols for sterol sulfate analysis using GC-MS. In this study we present a reinvestigation and evaluation of different deconjugation and derivatization procedures with a set of representative sterol sulfates. The advantages and disadvantages of trimethylsilyl (TMS), methyloxime-trimethylsilyl (MO-TMS), and trifluoroacetyl (TFA) derivatives were examined. Different published procedures of sterol sulfate deconjugation, including enzymatic and chemical cleavage, were reinvestigated and examined for diverse sterol sulfates. Finally, we present a new protocol for the chemical cleavage of sterol sulfates, allowing for simultaneous deconjugation and derivatization, simplifying GC-MS based sterol sulfate analysis.

Start of UK Confidential Haemophilia A Database: Analysis of 142 Patients by Solid Phase Fluorescent Chemical Cleavage of Mismatch

1999 ◽  
Vol 81 (06) ◽  
pp. 900-905 ◽  
Author(s):  
Naushin Waseem ◽  
Richard Bagnall ◽  
Peter Green ◽  
Francesco Giannelli ◽  
Keyword(s):  
Factor Viii ◽  
Solid Phase ◽  
Haemophilia A ◽  
National Strategy ◽  
Coding Region ◽  
Database Analysis ◽  
Chemical Cleavage ◽  
Signal Region ◽  
The Uk ◽  
Von Willebrand

SummaryA national strategy for optimising genetic services in haemophilia A has been initiated in the UK. Solid phase fluorescent chemical cleavage of mismatch is used to screen the entire coding region of factor VIII in six segments: four amplified from the trace of mRNA in blood lymphocytes and two from genomic DNA for the 3.4 kb exon 14 and flanking intron sequences. These segments are analysed in two threefold multiplexes so that the genes of 18 patients can be screened in a single ABI 377 gel. The promoter and polyadenylation signal region are amplified and sequenced directly. We have analysed 142 unrelated patients and identified 141 factor VIII mutations and one Normandy type von Willebrand homozygote. The former mutations include 89 missense, 10 nonsense, 5 frameshift, one 24 bp deletion and one splice signal defect. These comprise 71 different changes, of which 39 have not been previously observed.

ChemInform Abstract: Chemical Cleavage of Proteins at Methionyl-X Peptide Bonds

ChemInform ◽  
2010 ◽  
Vol 33 (50) ◽  
pp. no-no
Author(s):  
Bryan John Smith
Keyword(s):  
Peptide Bonds ◽  
Chemical Cleavage

Chemical Cleavage of Benzoyl Amino Acid Amides

Synthesis ◽  
1974 ◽  
Vol 1974 (04) ◽  
pp. 281-282 ◽  
Author(s):  
I. SHAHAK ◽  
Y. SASSON ◽  
Y. WOLMAN
Keyword(s):  
Amino Acid ◽  
Chemical Cleavage
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