Heterologous Expression of Membrane Proteins for Structural Analysis

2009 ◽  
pp. 1-16 ◽  
Author(s):  
Isabelle Mus-Veteau
Keyword(s):  
Structural Analysis ◽  
Membrane Proteins ◽  
Heterologous Expression

scholarly journals An Approach to Heterologous Expression of Membrane Proteins. The Case of Bacteriorhodopsin

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0128390 ◽  
Author(s):  
Dmitry Bratanov ◽  
Taras Balandin ◽  
Ekaterina Round ◽  
Vitaly Shevchenko ◽  
Ivan Gushchin ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Heterologous Expression

scholarly journals Heterologous Expression and Purification Systems for Structural Proteomics of Mammalian Membrane Proteins

2002 ◽  
Vol 3 (6) ◽  
pp. 511-517 ◽  
Author(s):  
Isabelle Mus-Veteau
Keyword(s):  
Membrane Proteins ◽  
Heterologous Expression ◽  
Large Scale ◽  
Structural Information ◽  
Structural Data ◽  
Structural Proteomics ◽  
Scale Production ◽  
Expression And Purification ◽  
Large Scale Production ◽  
Heterologous Expression Systems

Membrane proteins (MPs) are responsible for the interface between the exterior and the interior of the cell. These proteins are implicated in numerous diseases, such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, heart failure, hypertension and Alzheimer's disease. However, studies on these disorders are hampered by a lack of structural information about the proteins involved. Structural analysis requires large quantities of pure and active proteins. The majority of medically and pharmaceutically relevant MPs are present in tissues at very low concentration, which makes heterologous expression in large-scale production-adapted cells a prerequisite for structural studies. Obtaining mammalian MP structural data depends on the development of methods that allow the production of large quantities of MPs. This review focuses on the different heterologous expression systems, and the purification strategies, used to produce large amounts of pure mammalian MPs for structural proteomics.

scholarly journals The E. coli single protein production system for production and structural analysis of membrane proteins

2009 ◽  
Vol 11 (1) ◽  
pp. 81-84 ◽  
Author(s):  
Lili Mao ◽  
S. Thangminlal Vaiphei ◽  
Tsutomu Shimazu ◽  
William M. Schneider ◽  
Yuefeng Tang ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Membrane Proteins ◽  
Production System ◽  
Protein Production ◽  
E Coli ◽  
Single Protein

scholarly journals Perturbation of the yeast mitochondrial lipidome and associated membrane proteins following heterologous expression of Artemia-ANT

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Emily Chen ◽  
Michael A. Kiebish ◽  
Justice McDaniel ◽  
Katarzyna Niedzwiecka ◽  
Roza Kucharczyk ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Heterologous Expression

scholarly journals Electron structural analysis of membrane proteins

2001 ◽  
Vol 41 (supplement) ◽  
pp. S18
Author(s):  
K. Murata
Keyword(s):  
Structural Analysis ◽  
Membrane Proteins
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