Application of Cell Traction Force Microscopy for Cell Biology Research

2009 ◽  
pp. 301-313 ◽  
Author(s):  
James H-C. Wang ◽  
Bin Li
Keyword(s):  
Cell Biology ◽  
Traction Force ◽  
Traction Force Microscopy ◽  
Force Microscopy ◽  
Cell Traction ◽  
Biology Research

What Forces Does Cell Traction Force Microscopy Measure?

2011 ◽  
Author(s):  
Evan A. Zamir
Keyword(s):  
Inverse Problem ◽  
Cell Mechanics ◽  
Individual Cell ◽  
Traction Force ◽  
Cellular Level ◽  
Traction Force Microscopy ◽  
Force Microscopy ◽  
The World ◽  
Cell Traction ◽  
Rigorous Method

It is probably fair to say that the field of cell mechanics emerged with the pioneering work of Harris et al. [1], who observed that cells grown on thin silicone sheets generated wrinkling patterns — unfortunately, quantifying the forces at the cellular level was virtually impossible with their system. Almost two decades later, the study of cell mechanics began in earnest when Pelham and Wang [2] introduced a more rigorous method for quantifying individual cell-generated forces that quickly became known as cell traction force microscopy (CTFM), some form of which is now used in cell mechanics labs around the world. The basic idea underlying the original CTFM method is that the forces generated by cells can be calculated by solving an inverse problem for the displacement field experimentally measured by tracking microspheres embedded in a thin elastic substratum (typically polyacrylamide gel) on which the cells are cultured.

scholarly journals A Novel Cell Traction Force Microscopy to Study Multi-Cellular System

2014 ◽  
Vol 10 (6) ◽  
pp. e1003631 ◽  
Author(s):  
Xin Tang ◽  
Alireza Tofangchi ◽  
Sandeep V. Anand ◽  
Taher A. Saif
Keyword(s):  
Traction Force ◽  
Cellular System ◽  
Traction Force Microscopy ◽  
Force Microscopy ◽  
Cell Traction

scholarly journals 03-P012 Cell traction force microscopy in cardiac morphogenesis

2009 ◽  
Vol 126 ◽  
pp. S70-S71
Author(s):  
Veronika Boczonadi ◽  
Victoria Chen ◽  
Deborah Henderson ◽  
Bill Chaudhry
Keyword(s):  
Traction Force ◽  
Traction Force Microscopy ◽  
Cardiac Morphogenesis ◽  
Force Microscopy ◽  
Cell Traction

scholarly journals Investigation of human iPSC-derived cardiac myocyte functional maturation by single cell traction force microscopy

PLoS ONE ◽  
2018 ◽  
Vol 13 (4) ◽  
pp. e0194909 ◽  
Author(s):  
Matthew Wheelwright ◽  
Zaw Win ◽  
Jennifer L. Mikkila ◽  
Kamilah Y. Amen ◽  
Patrick W. Alford ◽  
...  
Keyword(s):  
Single Cell ◽  
Cardiac Myocyte ◽  
Traction Force ◽  
Traction Force Microscopy ◽  
Force Microscopy ◽  
Functional Maturation ◽  
Cell Traction ◽  
Human Ipsc

scholarly journals Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Liliana Barbieri ◽  
Huw Colin-York ◽  
Kseniya Korobchevskaya ◽  
Di Li ◽  
Deanna L. Wolfson ◽  
...  
Keyword(s):  
Resolution Enhancement ◽  
Traction Force ◽  
Super Resolution ◽  
Traction Force Microscopy ◽  
Two Dimensional ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Force Microscopy ◽  
Health And Disease ◽  
Spatio Temporal

AbstractQuantifying small, rapidly evolving forces generated by cells is a major challenge for the understanding of biomechanics and mechanobiology in health and disease. Traction force microscopy remains one of the most broadly applied force probing technologies but typically restricts itself to slow events over seconds and micron-scale displacements. Here, we improve >2-fold spatially and >10-fold temporally the resolution of planar cellular force probing compared to its related conventional modalities by combining fast two-dimensional total internal reflection fluorescence super-resolution structured illumination microscopy and traction force microscopy. This live-cell 2D TIRF-SIM-TFM methodology offers a combination of spatio-temporal resolution enhancement relevant to forces on the nano- and sub-second scales, opening up new aspects of mechanobiology to analysis.

scholarly journals Different Cell Migration Modes: Insights from Traction Force Microscopy and Modeling

2021 ◽  
Vol 120 (3) ◽  
pp. 113a
Author(s):  
Wouter-Jan Rappel ◽  
Elisabeth Ghabache ◽  
Yuansheng Cao ◽  
Yuchuan Miao ◽  
Alexander Groisman ◽  
...  
Keyword(s):  
Cell Migration ◽  
Traction Force ◽  
Traction Force Microscopy ◽  
Force Microscopy
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