Isolation of Transcription Factor Complexes from Arabidopsis Cell Suspension Cultures by Tandem Affinity Purification

2011 ◽  
pp. 195-218 ◽  
Author(s):  
Jelle Van Leene ◽  
Dominique Eeckhout ◽  
Geert Persiau ◽  
Eveline Van De Slijke ◽  
Jan Geerinck ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Cell Suspension ◽  
Affinity Purification ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Tandem Affinity Purification ◽  
Transcription Factor Complexes

scholarly journals A Generic Tool for Transcription Factor Target Gene Discovery in Arabidopsis Cell Suspension Cultures Based on Tandem Chromatin Affinity Purification

2014 ◽  
Vol 164 (3) ◽  
pp. 1122-1133 ◽  
Author(s):  
Aurine Verkest ◽  
Thomas Abeel ◽  
Ken S. Heyndrickx ◽  
Jelle Van Leene ◽  
Christa Lanz ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Cell Suspension ◽  
Target Gene ◽  
Affinity Purification ◽  
Gene Discovery ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Transcription Factor Target

scholarly journals Affinity Purification and Characterization of Functional Tubulin from Cell Suspension Cultures of Arabidopsis and Tobacco

2016 ◽  
Vol 170 (3) ◽  
pp. 1189-1205 ◽  
Author(s):  
Takashi Hotta ◽  
Satoshi Fujita ◽  
Seiichi Uchimura ◽  
Masahiro Noguchi ◽  
Taku Demura ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Affinity Purification ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Purification And Characterization

scholarly journals The Arabidopsis GRAS-type SCL28 transcription factor controls the mitotic cell cycle and division plane orientation

2021 ◽  
Vol 118 (6) ◽  
pp. e2005256118
Author(s):  
Camila Goldy ◽  
José-Antonio Pedroza-Garcia ◽  
Natalie Breakfield ◽  
Toon Cools ◽  
Rodrigo Vena ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Transcription Factor ◽  
Cell Suspension ◽  
Messenger Rna ◽  
Expression Patterns ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
M Cells ◽  
Root Tips

Gene expression is reconfigured rapidly during the cell cycle to execute the cellular functions specific to each phase. Studies conducted with synchronized plant cell suspension cultures have identified hundreds of genes with periodic expression patterns across the phases of the cell cycle, but these results may differ from expression occurring in the context of intact organs. Here, we describe the use of fluorescence-activated cell sorting to analyze the gene expression profile of G2/M cells in the growing root. To this end, we isolated cells expressing the early mitosis cell cycle marker CYCLINB1;1-GFP from Arabidopsis root tips. Transcriptome analysis of these cells allowed identification of hundreds of genes whose expression is reduced or enriched in G2/M cells, including many not previously reported from cell suspension cultures. From this dataset, we identified SCL28, a transcription factor belonging to the GRAS family, whose messenger RNA accumulates to the highest levels in G2/M and is regulated by MYB3R transcription factors. Functional analysis indicates that SCL28 promotes progression through G2/M and modulates the selection of cell division planes.

Transformation of Papaver somniferum cell suspension cultures with sam1 from A. thaliana results in cell lines of different S-adenosyl-L-methionine synthetase activity

1997 ◽  
Vol 99 (2) ◽  
pp. 233-240 ◽  
Author(s):  
Muriel Belny ◽  
Didier Herouart ◽  
Brigitte Thomasset ◽  
Helene David ◽  
Annie Jacquin-Dubreuil ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Cell Lines ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Papaver Somniferum ◽  
Synthetase Activity
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