Converting Monoclonal Antibodies into Fab Fragments for Transient Expression in Mammalian Cells

2011 ◽  
pp. 137-159 ◽  
Author(s):  
Joanne E. Nettleship ◽  
Aleksandra Flanagan ◽  
Nahid Rahman-Huq ◽  
Rebecca Hamer ◽  
Raymond J. Owens
Keyword(s):  
Monoclonal Antibodies ◽  
Transient Expression ◽  
Mammalian Cells ◽  
Fab Fragments ◽  
Expression In Mammalian Cells

Transient expression in mammalian cells of the bacterial reporter gene encoding mercuric reductase: effects of various regulatory elements

Gene ◽  
1988 ◽  
Vol 71 (2) ◽  
pp. 381-390 ◽  
Author(s):  
Gerrit Veldhuisen ◽  
Margriet Van Dijk ◽  
Jorieke Meijer ◽  
Betty E. Enger-Valk ◽  
Peter H. Pouwels
Keyword(s):  
Transient Expression ◽  
Reporter Gene ◽  
Mammalian Cells ◽  
Regulatory Elements ◽  
Gene Encoding ◽  
Mercuric Reductase ◽  
Expression In Mammalian Cells

High Performance Transient Expression in Mammalian Cells

2001 ◽  
pp. 83-87
Author(s):  
Randal A. Goffe ◽  
Adeelia S. Goffe ◽  
Tod J. Martin ◽  
Robert J. Rosler ◽  
Mel A. Mersberg ◽  
...  
Keyword(s):  
Transient Expression ◽  
Mammalian Cells ◽  
High Performance ◽  
Expression In Mammalian Cells

scholarly journals Transient Expression of a Recombinant Monoclonal Antibody in HEK293T Cells

2018 ◽  
Vol 24 (3) ◽  
pp. 207-212 ◽  
Author(s):  
Omid Mohammadian ◽  
Masoumeh Rajabibazl ◽  
Hadi Bayat ◽  
Azam Rahimpour
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Transient Expression ◽  
Mammalian Cells ◽  
Expression Vector ◽  
Expression System ◽  
Cell Culture Supernatant ◽  
Egfp Expression ◽  
Enzyme Analysis ◽  
Bicistronic Expression

Background: Monoclonal antibodies (mAbs) are considered the most important and financially successful category of the biopharmaceuticals. Extensive optimization of the expression vector, host system and culture parameters are required for the successful production of active monoclonal antibodies in mammalian cells. In this regards, transient expression enables rapid and cost-effective production of recombinant proteins for initial characterization. Methods: In the present study, an internal ribosome entry site (IRES) based bicistronic expression system has been evaluated for the transient expression of an anti-CD52 monoclonal antibody in mammalian cells. The IRES based bicistronic vector was generated through sequential cloning of the Light chain (LC), IRES, and Heavy chain (HC) in an intermediate vector and transfer of the resulting fragment to the expression vector. Transfection of the HEK293T cells was performed and antibody expression was analyzed in cell culture supernatant. Results: Restriction enzyme analysis indicated successful cloning of the antibody coding unit in the expression vector. Analysis of EGFP expression indicated successful transfection of the HEK293T cells. Production levels of 220 µg/L of antibody were achieved in HEK293T cells during three days of culture. Conclusion: Our results show the convenience and efficiency of the bicistronic expression system for transient expression of the whole monoclonal antibodies in mammalian cells.

scholarly journals Transient expression in mammalian cells of transgenes transcribed from theCauliflower mosaic virus35S promoter

2004 ◽  
Vol 3 (2) ◽  
pp. 91-97 ◽  
Author(s):  
Mark Tepfer ◽  
Stéphane Gaubert ◽  
Mathieu Leroux-Coyau ◽  
Sonia Prince ◽  
Louis-Marie Houdebine
Keyword(s):  
Transient Expression ◽  
Mammalian Cells ◽  
Expression In Mammalian Cells

scholarly journals Optimization of Codon Usage of Poxvirus Genes allows for Improved Transient Expression in Mammalian Cells

Virus Genes ◽  
2006 ◽  
Vol 33 (1) ◽  
pp. 15-26 ◽  
Author(s):  
John W. Barrett ◽  
Yunming Sun ◽  
Steven H. Nazarian ◽  
Tara A. Belsito ◽  
Craig R. Brunetti ◽  
...  
Keyword(s):  
Codon Usage ◽  
Transient Expression ◽  
Mammalian Cells ◽  
Expression In Mammalian Cells
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