High Level Transient Expression in Mammalian Cells: Identification and Optimization of Physico-Chemical Parameters of the Calcium Phosphate Transfection Method

1995 ◽  
pp. 49-55 ◽  
Author(s):  
Martin Jordan ◽  
Florian M. Wurm
Keyword(s):  
Calcium Phosphate ◽  
Transient Expression ◽  
Mammalian Cells ◽  
Chemical Parameters ◽  
Physico Chemical ◽  
Transfection Method ◽  
High Level ◽  
Expression In Mammalian Cells ◽  
Calcium Phosphate Transfection

scholarly journals Versatile multi-transgene expression using improved BAC TG-EMBED toolkit, novel BAC episomes, and BAC-MAGIC

2019 ◽  
Author(s):  
Binhui Zhao ◽  
Pankaj Chaturvedi ◽  
David L. Zimmerman ◽  
Andrew S. Belmont
Keyword(s):  
Mammalian Cells ◽  
Transgene Expression ◽  
Large Fraction ◽  
Fine Tuning ◽  
Cmv Promoter ◽  
Chromosome Position ◽  
High Level ◽  
Genomic Regions ◽  
Expression In Mammalian Cells

ABSTRACTAchieving reproducible, stable, and high-level transgene expression in mammalian cells remains problematic. Previously, we attained copy-number-dependent, chromosome-position-independent expression of reporter minigenes by embedding them within a BAC containing the mouseMsh3-Dhfrlocus (DHFR BAC). Here we extend this “BAC TG-EMBED” approach. First, we report a toolkit of endogenous promoters capable of driving transgene expression over a 0.01-5 fold expression range relative to the CMV promoter, allowing fine-tuning of relative expression levels of multiple reporter genes expressed on a single BAC. Second, we show small variability in both the expression level and long-term expression stability of a reporter gene embedded in BACs containing either transcriptionally active or inactive genomic regions, making choice of BACs more flexible. Third, we describe an intriguing phenomenon in which BAC transgenes are maintained as episomes in a large fraction of stably selected clones. Finally, we demonstrate the utility of BAC TG-EMBED by simultaneously labeling three nuclear compartments in 94% of stable clones using a multi-reporter DHFR BAC, constructed with a combination of synthetic biology and BAC recombineering tools. Our extended BAC TG-EMBED method provides a versatile platform for achieving reproducible, stable simultaneous expression of multiple transgenes maintained either as episomes or stably integrated copies.

scholarly journals Spatial and Monthly Changes in Interstitial Water Physico-Chemistry of Bodo Creek

2021 ◽  
pp. 1-8
Author(s):  
D. K. Nkeeh ◽  
A. I. Hart ◽  
E. S. Erondu ◽  
N. Zabbey
Keyword(s):  
Oxygen Demand ◽  
Aquatic Organisms ◽  
Ion Concentration ◽  
Chemical Parameters ◽  
Monthly Basis ◽  
Hydrogen Ion Concentration ◽  
Quality Of Water ◽  
Physico Chemical ◽  
Significant Difference ◽  
High Level

Water plays a key role in the survival, growth and reproduction of aquatic organisms. Therefore maintaining good quality of water physico-chemical parameters would ensure optimum productivity of aquatic fauna. The study evaluated water physical and chemical parameters (temperature, hydrogen ion concentration (pH), conductivity, dissolved oxygen (DO) and biochemical oxygen demand (BOD) of Bodo Creek. Sample was collected for 5 months (July 2020 – November 2020) at locations previously studied (before-spill baseline studies, 3 years, 5 years, and 7 years after-spill); by using the sampling methods used in previous studies. Spatially, there was no statistically significant difference in temperature (p > 0.05 = 0.997), pH (p > 0.05 = 0.496), DO (p > 0.05= 0.34) and BOD (p > 0.05 = 0.644). However, the difference in conductivity between the stations was statistically significant (p < 0.05 = 0.006). On monthly basis, there was a significant difference in temperature (p < 0.05 = 0.000) and pH (p < 0.05 = 0.005). In contrast, there was no statistically significant difference in conductivity (p > 0.05 = 0.633), DO (p > 0.05 = 0.559) and BOD (p > 0.05 = 0.75). Most of the physico-chemical parameters were not within the WHO recommended levels for the survival of aquatic lives, indicating a high level of environmental pollution in Bodo Creek. This study provides dataset for future evaluation of the water physico-chemistry of Bodo Creek.

High-Level Expression in Mammalian Cells of Recombinant House Dust Mite Allergen ProDer p 1 with Optimized Codon Usage

2001 ◽  
Vol 125 (1) ◽  
pp. 32-43 ◽  
Author(s):  
Marc Massaer ◽  
Pasqualina Mazzu ◽  
Michèle Haumont ◽  
Mauro Magi ◽  
Véronique Daminet ◽  
...  
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Mammalian Cells ◽  
Mite Allergen ◽  
High Level Expression ◽  
House Dust Mite Allergen ◽  
Dust Mite ◽  
Dust Mite Allergen ◽  
High Level ◽  
Expression In Mammalian Cells

scholarly journals Comparison of some physico-chemical parameters of kiwiberry (Actinidia arguta) cultivars from a cold climate

2019 ◽  
Vol 31 (2) ◽  
pp. 375-383 ◽  
Author(s):  
Miroslav Horák ◽  
Petr Šnurkovič ◽  
Ivo Ondrášek ◽  
Josef Balík ◽  
Varit Srilaong
Keyword(s):  
Ascorbic Acid Content ◽  
Cold Climate ◽  
Chemical Parameters ◽  
Actinidia Arguta ◽  
Fruit Species ◽  
Physico Chemical ◽  
Predominant Type ◽  
Fruit Skin ◽  
Wide Range ◽  
High Level

AbstractThe Actinidia genus includes a number of commercial fruit species with a wide range of countries of origin. Some species such as A. arguta or A. kolomikta are characterized by a high level of resistance to frost. The aim of this study was to compare the fruit of selected cultivars of kiwiberry. In the kiwiberry fruit, sucrose was the predominant type of sugar. Citric acid was dominant in all samples; it was most represented in the pulp of kiwiberry seedlings and in the skin of kiwiberry ‘Issai’. Kiwiberry fruits feature a higher ascorbic acid content in the skin in comparison with the commercial A. chinensis cultivars. Antioxidant activity of kiwiberry fruit skin was up to 18 times higher than that of the pulp. In view of the kiwiberry fruit having no hair, it is possible to eat the whole fruit, including the skin containing substances beneficial to health.

[5] Stable high-level gene expression in mammalian cells by T7 phage RNA polymerase

1993 ◽  
pp. 47-66 ◽  
Author(s):  
Andre Lieber ◽  
Volker Sandig ◽  
Wolfgang Sommer ◽  
Silvia Bähring ◽  
Michael Strauss
Keyword(s):  
Gene Expression ◽  
Rna Polymerase ◽  
Mammalian Cells ◽  
High Level ◽  
T7 Phage ◽  
Expression In Mammalian Cells
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