Exploring the Link Between Human Embryonic Stem Cell Organization and Fate Using Tension-Calibrated Extracellular Matrix Functionalized Polyacrylamide Gels

2012 ◽  
pp. 317-350 ◽  
Author(s):  
Johnathon N. Lakins ◽  
Andrew R. Chin ◽  
Valerie M. Weaver
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Polyacrylamide Gels ◽  
Cell Organization

scholarly journals Recombinant Extracellular Matrix Protein Fragments Support Human Embryonic Stem Cell Chondrogenesis

2018 ◽  
Vol 24 (11-12) ◽  
pp. 968-978 ◽  
Author(s):  
Aixin Cheng ◽  
Stuart A. Cain ◽  
Pinyuan Tian ◽  
Andrew K. Baldwin ◽  
Paweena Uppanan ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Matrix Protein ◽  
Embryonic Stem ◽  
Extracellular Matrix Protein ◽  
Protein Fragments

Identification of the Critical Extracellular Matrix Proteins that Promote Human Embryonic Stem Cell Assembly

2009 ◽  
Vol 18 (6) ◽  
pp. 919-928 ◽  
Author(s):  
Denis Evseenko ◽  
Katja Schenke-Layland ◽  
Gautam Dravid ◽  
Yuhua Zhu ◽  
Qian-Lin Hao ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Cell Assembly

Extracellular Matrix Isolated From Foreskin Fibroblasts Supports Long-Term Xeno-Free Human Embryonic Stem Cell Culture

2010 ◽  
Vol 19 (4) ◽  
pp. 547-556 ◽  
Author(s):  
Guoliang Meng ◽  
Shiying Liu ◽  
Xiangyun Li ◽  
Roman Krawetz ◽  
Derrick E. Rancourt
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Culture

scholarly journals Extracellular matrix formation after transplantation of human embryonic stem cell-derived cardiomyocytes

2009 ◽  
Vol 67 (2) ◽  
pp. 277-290 ◽  
Author(s):  
L. W. van Laake ◽  
E. G. van Donselaar ◽  
J. Monshouwer-Kloots ◽  
C. Schreurs ◽  
R. Passier ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem

scholarly journals Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix

2020 ◽  
Vol 11 ◽  
pp. 204173142092148 ◽  
Author(s):  
Camila Hochman-Mendez ◽  
Dilza Balteiro Pereira de Campos ◽  
Rafael Serafim Pinto ◽  
Bernardo Jorge da Silva Mendes ◽  
Gustavo Miranda Rocha ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Connexin 43 ◽  
Human Embryonic Stem Cell ◽  
Cardiac Tissue ◽  
Electrical Coupling ◽  
Embryonic Stem ◽  
Biochemical Properties ◽  
Extracellular Matrix Scaffolds

Decellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex cardiac extracellular matrix. However, evaluating the extent of cardiomyocyte repopulation of decellularized cardiac extracellular matrix scaffolds after recellularization attempts is challenging. Here, we describe a unique combination of biochemical, biomechanical, histological, and physiological parameters for quantifying recellularization efficiency of tissue-engineered cardiac patches compared with native cardiac tissue. Human embryonic stem cell-derived cardiomyocytes were seeded into rat heart atrial and ventricular decellularized cardiac extracellular matrix patches. Confocal and atomic force microscopy showed cell integration within the extracellular matrix basement membrane that was accompanied by restoration of native cardiac tissue passive mechanical properties. Multi-electrode array and immunostaining (connexin 43) were used to determine synchronous field potentials with electrical coupling. Myoglobin content (~60%) and sarcomere length measurement (>45% vs 2D culture) were used to evaluate cardiomyocyte maturation of integrated cells. The combination of these techniques allowed us to demonstrate that as cellularization efficiency improves, cardiomyocytes mature and synchronize electrical activity, and tissue mechanical/biochemical properties improve toward those of native tissue.

IGF1-mediated human embryonic stem cell self-renewal recapitulates the embryonic niche

2020 ◽  
Author(s):  
Wamaitha SE ◽  
Grybel KJ ◽  
Alanis-Lobato G ◽  
Gerri C ◽  
Ogushi S ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Self Renewal
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