The Hemizona Assay for Assessment of Sperm Function

The ejaculate is analyzed in the clinic primarily to obtain an estimation of sperm number, motion characteristics and cell morphology. Based on these data, conclusions are formulated as to the overall ‘fertility’ of the male. A major problem has been that the conclusions derived are often not (well) correlated with expected outcome, i.e., fertility. As a consequence, adjunct tests, e.g., sperm function tests, have been developed in an effort to uncover those defects/assets not revealed when measuring standard semen parameters. While some of these tests, e.g., hemizona assay, have helped to shed light on dysfunctional processes associated with fertilization, e.g., zona binding and penetration, individually they do not totally suffice for identifying fertile (defined as being capable of producing offspring) males.

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Levels of reactive oxygen species (ROS) in the seminal plasma predicts the effectiveness of L-carnitine to improve sperm function in men with infertility

Endocrine Abstracts ◽

10.1530/endoabs.44.p232 ◽

2016 ◽

Cited By ~ 2

Author(s):

Wayne Vessey ◽

Cassandra McDonald ◽

Ashraf Virmani ◽

P. Almeida ◽

Channa Jayasena ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Seminal Plasma ◽

Reactive Oxygen ◽

Sperm Function ◽

Oxygen Species

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Levels of reactive oxygen species (ROS) in the seminal plasma predicts the effectiveness of L-carnetine to improve sperm function in men with infertility

10.26226/morressier.573c1510d462b80296c980ee ◽

2016 ◽

Author(s):

Wayne Vessey

Keyword(s):

Reactive Oxygen Species ◽

Seminal Plasma ◽

Reactive Oxygen ◽

Sperm Function ◽

Oxygen Species

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Oxidative stress and role of antioxidants in normal and abnormal sperm function

Frontiers in Bioscience ◽

10.2741/a146 ◽

1996 ◽

Vol 1 (5) ◽

pp. e78-86 ◽

Cited By ~ 194

Author(s):

S. C Sikka

Keyword(s):

Oxidative Stress ◽

Sperm Function ◽

Abnormal Sperm

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Sperm Competition in the Absence of Fertilization in Caenorhabditis elegans

Genetics ◽

10.1093/genetics/152.1.201 ◽

1999 ◽

Vol 152 (1) ◽

pp. 201-208 ◽

Cited By ~ 2

Author(s):

Andrew Singson ◽

Katherine L Hill ◽

Steven W L’Hernault

Keyword(s):

Caenorhabditis Elegans ◽

Sperm Competition ◽

Sperm Function ◽

Sperm Precedence ◽

Wild Type ◽

Nematode Caenorhabditis Elegans ◽

C Elegans ◽

Normal Sperm ◽

Self Fertilization ◽

Competition Assays

Abstract Hermaphrodite self-fertilization is the primary mode of reproduction in the nematode Caenorhabditis elegans. However, when a hermaphrodite is crossed with a male, nearly all of the oocytes are fertilized by male-derived sperm. This sperm precedence during reproduction is due to the competitive superiority of male-derived sperm and results in a functional suppression of hermaphrodite self-fertility. In this study, mutant males that inseminate fertilization-defective sperm were used to reveal that sperm competition within a hermaphrodite does not require successful fertilization. However, sperm competition does require normal sperm motility. Additionally, sperm competition is not an absolute process because oocytes not fertilized by male-derived sperm can sometimes be fertilized by hermaphrodite-derived sperm. These results indicate that outcrossed progeny result from a wild-type cross because male-derived sperm are competitively superior and hermaphrodite-derived sperm become unavailable to oocytes. The sperm competition assays described in this study will be useful in further classifying the large number of currently identified mutations that alter sperm function and development in C. elegans.

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