Chiral Separations by HPLC on Immobilized Polysaccharide Chiral Stationary Phases

2012 ◽  
pp. 127-135 ◽  
Author(s):  
Imran Ali ◽  
Zeid A. AL-Othman ◽  
Hassan Y. Aboul-Enein
Keyword(s):  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Chiral Separations

Chiral separations of β-blocking drug substances using chiral stationary phases

1995 ◽  
Vol 708 (2) ◽  
pp. 253-261 ◽  
Author(s):  
Jens Ekelund ◽  
Anja van Arkens ◽  
Kirsten Brønnum-Hansen ◽  
Karen Fich ◽  
Lars Olsen ◽  
...  
Keyword(s):  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Chiral Separations ◽  
Blocking Drug ◽  
Drug Substances

New insights into supercritical fluid chromatography for chiral separations

2017 ◽  
Vol 9 (17) ◽  
pp. 2603-2610 ◽  
Author(s):  
Hermes Licea-Perez ◽  
Christopher A. Evans
Keyword(s):  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Chiral Separations ◽  
Chiral Resolution

Supercritical fluid chromatography in combination with chiral stationary phases has proven to be a great tool for chiral resolution, alleviating some of the challenges associated with bioanalysis of stereoisomers.

Enantioseparation of 3α-acyloxy-6β-acetoxyltropane compounds with Chiralpak AD and Chiralcel OD-H chiral stationary phases

2015 ◽  
Vol 33 (6) ◽  
pp. 647
Author(s):  
Bin CHENG ◽  
Yifan XIE ◽  
Youmin HU ◽  
Huizhong LIU ◽  
Yinyao NIU ◽  
...  
Keyword(s):  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Chiralcel Od

scholarly journals Recent Advances in Chiral Analysis of Proteins and Peptides

Separations ◽  
2021 ◽  
Vol 8 (8) ◽  
pp. 112
Author(s):  
Marine Morvan ◽  
Ivan Mikšík
Keyword(s):  
Amino Acids ◽  
Stationary Phases ◽  
Enzymatic Digestion ◽  
Chiral Separations ◽  
Biological Compounds ◽  
Health And Disease ◽  
Electrophoretic Techniques ◽  
A Site ◽  
Future Work ◽  
Proteins And Peptides

Like many biological compounds, proteins are found primarily in their homochiral form. However, homochirality is not guaranteed throughout life. Determining their chiral proteinogenic sequence is a complex analytical challenge. This is because certain D-amino acids contained in proteins play a role in human health and disease. This is the case, for example, with D-Asp in elastin, β-amyloid and α-crystallin which, respectively, have an action on arteriosclerosis, Alzheimer's disease and cataracts. Sequence-dependent and sequence-independent are the two strategies for detecting the presence and position of D-amino acids in proteins. These methods rely on enzymatic digestion by a site-specific enzyme and acid hydrolysis in a deuterium or tritium environment to limit the natural racemization of amino acids. In this review, chromatographic and electrophoretic techniques, such as LC, SFC, GC and CE, will be recently developed (2018–2020) for the enantioseparation of amino acids and peptides. For future work, the discovery and development of new chiral stationary phases and derivatization reagents could increase the resolution of chiral separations.

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