Quantification of Cyclic Dinucleotides by Reversed-Phase LC-MS/MS

2013 ◽  
pp. 27-37 ◽  
Author(s):  
Heike Burhenne ◽  
Volkhard Kaever
Keyword(s):  
Reversed Phase ◽  
Cyclic Dinucleotides ◽  
Reversed Phase Lc

scholarly journals Quantitation of cyclic dinucleotides by reversed-phase LC-MS/MS

2009 ◽  
Vol 9 (S1) ◽  
Author(s):  
Christian Spangler ◽  
Roland Seifert ◽  
Volkhard Kaever
Keyword(s):  
Reversed Phase ◽  
Cyclic Dinucleotides ◽  
Reversed Phase Lc

scholarly journals Quantitation of Multiple Sphingolipid Classes Using Normal and Reversed-Phase LC–ESI–MS/MS: Comparative Profiling of Two Cell Lines

Lipids ◽  
2011 ◽  
Vol 47 (2) ◽  
pp. 209-226 ◽  
Author(s):  
M. Athar Masood ◽  
Raghavendra P. Rao ◽  
Jairaj K. Acharya ◽  
Josip Blonder ◽  
Timothy D. Veenstra
Keyword(s):  
Cell Lines ◽  
Reversed Phase ◽  
Esi Ms ◽  
Reversed Phase Lc

Metal ion-mobilizing additives for comprehensive detection of femtomole amounts of phosphopeptides by reversed phase LC-MS

Amino Acids ◽  
2010 ◽  
Vol 41 (2) ◽  
pp. 311-320 ◽  
Author(s):  
Joerg Seidler ◽  
Nico Zinn ◽  
Erik Haaf ◽  
Martin E. Boehm ◽  
Dominic Winter ◽  
...  
Keyword(s):  
Metal Ion ◽  
Reversed Phase ◽  
Reversed Phase Lc

Determination of Metsulfuron Methyl and Its Two Metabolites in Crops by Liquid Chromatography with Ultraviolet Detection

1994 ◽  
Vol 77 (6) ◽  
pp. 1654-1659 ◽  
Author(s):  
Mjn Zhou ◽  
Gui-Yun Li ◽  
Stephanie A Whalen
Keyword(s):  
Liquid Chromatography ◽  
Signal To Noise Ratio ◽  
Reversed Phase ◽  
Metsulfuron Methyl ◽  
Sugarcane Stalk ◽  
Simple Extraction ◽  
Oat Straw ◽  
Reversed Phase Lc ◽  
Hydroxy Metabolite

Abstract Metsulfuron methyl, its hydroxy metabolite (A1), and its glucose conjugate metabolite (A) were determined in several crops by liquid chromatography (LC) using a simple extraction and cleanup scheme. After the parent and 2 major metabolites were extracted from crops with methanol, metabolite A was enzymatically hydrolyzed to metabolite A1 with β-glucosidase. The treated extracts were cleaned up chromatographically, and then metsulfuron methyl and metabolite A1 were quantitatively determined by reversed-phase LC with UV detection at 254 nm. Recovery of metsulfuron methyl and its 2 metabolites was 90 ± 6% over a range of 0.005 to 0.4 ppm from fortified samples of brown grain, wheat, barley, sugarcane stalk, and oat straw. Method detection limits (MDLs) were 0.005 ppm for metsulfuron methyl, 0.006 ppm for metabolite A, and 0.003 ppm for metabolite A1 in such crops as brown grain, wheat, barley, and sugarcane, and 0.01 ppm for metsulfuron methyl, 0.015 ppm for metabolite A, and 0.01 ppm for metabolite A1 in oat straw. The MDLs were estimated on the basis of a signal-to-noise ratio of 5 within a confidence interval of 95%. The method has potential application for the analyses of these analytes in other crops and feeds and may be applicable to other sulfonylurea herbicides.

scholarly journals Liquid Chromatographic Method for Determining the Concentration of Bisazir in Water

1997 ◽  
Vol 80 (5) ◽  
pp. 1111-1116 ◽  
Author(s):  
Ronald J Scholefield ◽  
Karen S Slaght ◽  
John L Allen
Keyword(s):  
Great Lakes ◽  
Solid Phase ◽  
Reversed Phase ◽  
Lake Huron ◽  
Extraction Column ◽  
Treated Effluent ◽  
Liquid Chromatographic Method ◽  
Sea Lampreys ◽  
Liquid Chromatographic ◽  
Reversed Phase Lc

Abstract Barrier dams, traps, and lampricides are the techniques currently used by the Great Lakes Fishery Commission to control sea lampreys {Petromyzon marinug) in the Great Lakes. To augment these control techniques, a sterile-male-release research program was initiated at the Lake Huron Biological Station. Male sea lampreys were sterilized by intraperitoneal injection of the chemical sterilant P,P-bis(1-aziridinyl)-Nmethylphosphinothioic amide (bisazir). An analytical method was needed to quantitate the concentration of bisazir in water and to routinely verify that bisazir (>25 μg/L) does not persist in the treated effluent discharged from the sterilization facility to Lake Huron. A rapid, accurate, and sensitive liquid chromatographic (LC) method was developed for determining bisazir in water. Bisazir was dissolved in Lake Huron water; extracted and concentrated on a C18 solid-phase extraction column; eluted with methanol; and quantitated by reversed-phase LC using a Cis column, amobile phase of 70% water and 30% methanol (v/v), and UV detection (205 nm). Bisazir retention time was 7-8 min; total run time was about 20 min. Method detection limit for bisazir dissolved in Lake Huron water was about 15 μg/L. Recovery from Lake Huron water fortified with bisazir at 100 μg/L was 94% (95% confidence interval, 90.2-98.2%).

Sign in / Sign up

Export Citation Format

Share Document