The first chapter lays a foundation for the book by defining boundary discourse in crossdisciplinary and cross-sector work. It begins by distinguishing spatial and organic metaphors of boundaries, with initial emphasis on disciplines. It then combines the two metaphors in a composite concept of an ecology of spatializing practices, illustrated by the evolving nature of disciplines as well as trading zones and communities of practice. The chapter then describes structures for interdisciplinary work, followed by the concept of heterarchy, changing character of higher education, platforms for communication and collaboration, and role of the built environment. It turns next to boundary objects, illustrated by construction of a natural history museum, an academic reform initiative, a project on waste management, and the relationship of objects and their description in climate modeling, regulatory discourse, genetic toxicology, and human ecology. The chapter ends by examining boundary organizations and agents in two cross-sector case studies.