Immobilization of Enzymes on Magnetic Beads Through Affinity Interactions

2013 ◽  
pp. 139-148 ◽  
Author(s):  
Audrey Sassolas ◽  
Akhtar Hayat ◽  
Jean-Louis Marty
Keyword(s):  
Magnetic Beads ◽  
Immobilization Of Enzymes

A new, efficient way of releasing cells from magnetic beads

1997 ◽  
Vol 56 (1-3) ◽  
pp. 375
Author(s):  
M Odnakk
Keyword(s):  
Magnetic Beads

Microsatellite Molecular Marker Enrichment by Magnetic Beads in Flax

2009 ◽  
Vol 34 (12) ◽  
pp. 2099-2105
Author(s):  
Xin DENG ◽  
Xin-Bo CHEN ◽  
Song-Hua LONG ◽  
Xiao-Chun WANG ◽  
Yuan GAO ◽  
...  
Keyword(s):  
Molecular Marker ◽  
Magnetic Beads

Isolation of Phagosomes from Dendritic Cells by Using Magnetic Beads

BIO-PROTOCOL ◽  
2013 ◽  
Vol 3 (14) ◽  
Author(s):  
Bénédicte Manoury
Keyword(s):  
Dendritic Cells ◽  
Magnetic Beads

Lockdown dilemma: ingestion of magnetic beads presenting as right iliac fossa pain and subacute small bowel obstruction

2020 ◽  
Vol 13 (11) ◽  
pp. e236429
Author(s):  
Bankole Oyewole ◽  
Anu Sandhya ◽  
Ian Maheswaran ◽  
Timothy Campbell-Smith
Keyword(s):  
Abdominal Pain ◽  
Small Bowel ◽  
Magnetic Beads ◽  
Iliac Fossa ◽  
Serous Fluid ◽  
Magnetic Resonance Scan ◽  
History Of ◽  
Right Iliac Fossa Pain ◽  
Multiple Episodes ◽  
Safety Netting

A 13-year-old girl presented with a 3-day history of migratory right iliac fossa pain. Observations and inflammatory markers were normal, and an ultrasound scan was inconclusive. A provisional diagnosis of non-specific abdominal pain or early appendicitis was made, and she was discharged with safety netting advice. She presented again 6 days later with ongoing abdominal pain now associated with multiple episodes of vomiting; hence, the decision was made to proceed to diagnostic laparoscopy rather than a magnetic resonance scan for further assessment. Intraoperative findings revealed 200 mL of serous fluid in the pelvis, normal-looking appendix, dilated stomach and a tangle of small bowel loops. Blunt and careful dissection revealed fistulous tracts that magnetised the laparoscopic instruments. A minilaparotomy was performed with the extraction of 14 magnetic beads and the repair of nine enterotomies. This case highlights the importance of careful history taking in children presenting with acute abdominal pain of doubtful aetiology.

scholarly journals Comparing the Use of Magnetic Beads with Ultrafiltration for Ancient Dental Calculus Proteomics

2021 ◽  
Vol 20 (3) ◽  
pp. 1689-1704
Author(s):  
Karren S. Palmer ◽  
Cheryl A. Makarewicz ◽  
Alexey A. Tishkin ◽  
Svetlana S. Tur ◽  
Amartuvshin Chunag ◽  
...  
Keyword(s):  
Magnetic Beads ◽  
Dental Calculus

scholarly journals Towards a Novel Computer-Aided Optimization of Microreactors: Techno-Economic Evaluation of an Immobilized Enzyme System

Symmetry ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 524
Author(s):  
Philip Pietrek ◽  
Manfred Kraut ◽  
Roland Dittmeyer
Keyword(s):  
Magnetic Beads ◽  
Glucose Dehydrogenase ◽  
Packed Bed ◽  
Detailed Knowledge ◽  
Reaction Conditions ◽  
Flow Reactors ◽  
Continuous Flow Reactors ◽  
Enzyme Cascades ◽  
And Control ◽  
Physical And Chemical

Immobilized multi-enzyme cascades are increasingly used in microfluidic devices. In particular, their application in continuous flow reactors shows great potential, utilizing the benefits of reusability and control of the reaction conditions. However, capitalizing on this potential is challenging and requires detailed knowledge of the investigated system. Here, we show the application of computational methods for optimization with multi-level reactor design (MLRD) methodology based on the underlying physical and chemical processes. We optimize a stereoselective reduction of a diketone catalyzed by ketoreductase (Gre2) and Nicotinamidadenindinukleotidphosphat (NADPH) cofactor regeneration with glucose dehydrogenase (GDH). Both enzymes are separately immobilized on magnetic beads forming a packed bed within the microreactor. We derive optimal reactor feed concentrations and enzyme ratios for enhanced performance and a basic economic model in order to maximize the techno-economic performance (TEP) for the first reduction of 5-nitrononane-2,8-dione.

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