scholarly journals Comparing the Use of Magnetic Beads with Ultrafiltration for Ancient Dental Calculus Proteomics

2021 ◽  
Vol 20 (3) ◽  
pp. 1689-1704
Author(s):  
Karren S. Palmer ◽  
Cheryl A. Makarewicz ◽  
Alexey A. Tishkin ◽  
Svetlana S. Tur ◽  
Amartuvshin Chunag ◽  
...  
1997 ◽  
Vol 56 (1-3) ◽  
pp. 375
Author(s):  
M Odnakk
Keyword(s):  

1978 ◽  
Vol 20 (4) ◽  
pp. 669-689
Author(s):  
Shohei HIGASHI ◽  
Tetsuo KODAKA ◽  
Miyuki KOBAYASHI ◽  
Tetsuko IMURA ◽  
Hiroaki NAKAGAWA

2020 ◽  
Author(s):  
Darko Stojanovski ◽  
Ivana Živaljević ◽  
Vesna Dimitrijević ◽  
Julie Dunne ◽  
Richard Evershed ◽  
...  

The application of biomolecular techniques to archaeological materials from the Balkans is providing valuable new information on the prehistory of the region. This is especially relevant for the study of the neolithisation process in SE Europe, which gradually affected the rest of the continent. Here, to answer questions regarding diet and subsistence practices in early farming societies, we combine organic residue analyses of archaeological pottery, taxonomic and isotopic study of domestic animal remains and biomolecular analyses of human dental calculus. The results from the analyses of the lipid residues from pottery suggest that milk was processed in ceramic vessels. Dairy products were shown to be part of the subsistence strategies of the earliest Neolithic communities in the region but were of varying importance in different areas of the Balkan. On the other hand, we did not confidently detect any milk proteins within the dental calculus. The molecular and isotopic identification of meat, dairy, plants and beeswax in the pottery lipids also provided insights into the diversity of diet in these early Neolithic communities. We also present the first compound-specific radiocarbon dates for the region, obtained directly on absorbed organic residues extracted from pottery, identified as dairy lipids.


2009 ◽  
Vol 34 (12) ◽  
pp. 2099-2105
Author(s):  
Xin DENG ◽  
Xin-Bo CHEN ◽  
Song-Hua LONG ◽  
Xiao-Chun WANG ◽  
Yuan GAO ◽  
...  

BIO-PROTOCOL ◽  
2013 ◽  
Vol 3 (14) ◽  
Author(s):  
Bénédicte Manoury

Author(s):  
Raphael Hirata Júnior

Resumo: O cálculo dentário é uma estrutura calcificada a partir do biofilme dentário (placa bacteriana) que, após o seu estabelecimento, é de difícil remoção e constitui um mecanismo de patogenicidade importante em diversas situações patológicas da cavidade oral por estar sempre coberto por uma camada viável de biofilme não mineralizada. A presente revisão de literatura releva a importância dos biofilmes calcificados nos contextos de doença, seus aspectos clínicos e microbiológicos em tempos anciães e contemporâneos, e sobre as medidas terapêuticas e preventivas. Apesar de todo conhecimento gerado sobre as doenças da cavidade oral, ainda são limitadas as abordagens clínicas a respeito dos mecanismos de controle dos cálculos dentários.Abstract: Dental calculus is a calcified structure from the dental biofilm (plaque), which, after its establishment, is difficult to remove and constitutes an important mechanism of pathogenicity in various pathological situations of the oral cavity because it is always covered by a viable layer of non-mineralized biofilm. The present literature highlights the importance of calcified biofilms in disease contexts, clinical and microbiological of dental calculus aspects in ancient and contemporary eras, and in therapeutic and preventive measures. Despite all the knowledge generated about the diseases of the oral cavity, the clinical approaches regarding the mechanisms of control of dental calculus still need investigations.Palavras Chave: Cálculo dentário, Calcificação do biofilme, Higiene oralKey words: Dental calculus, Biofilm calcification, Oral hygiene


2020 ◽  
Vol 13 (11) ◽  
pp. e236429
Author(s):  
Bankole Oyewole ◽  
Anu Sandhya ◽  
Ian Maheswaran ◽  
Timothy Campbell-Smith

A 13-year-old girl presented with a 3-day history of migratory right iliac fossa pain. Observations and inflammatory markers were normal, and an ultrasound scan was inconclusive. A provisional diagnosis of non-specific abdominal pain or early appendicitis was made, and she was discharged with safety netting advice. She presented again 6 days later with ongoing abdominal pain now associated with multiple episodes of vomiting; hence, the decision was made to proceed to diagnostic laparoscopy rather than a magnetic resonance scan for further assessment. Intraoperative findings revealed 200 mL of serous fluid in the pelvis, normal-looking appendix, dilated stomach and a tangle of small bowel loops. Blunt and careful dissection revealed fistulous tracts that magnetised the laparoscopic instruments. A minilaparotomy was performed with the extraction of 14 magnetic beads and the repair of nine enterotomies. This case highlights the importance of careful history taking in children presenting with acute abdominal pain of doubtful aetiology.


Symmetry ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 524
Author(s):  
Philip Pietrek ◽  
Manfred Kraut ◽  
Roland Dittmeyer

Immobilized multi-enzyme cascades are increasingly used in microfluidic devices. In particular, their application in continuous flow reactors shows great potential, utilizing the benefits of reusability and control of the reaction conditions. However, capitalizing on this potential is challenging and requires detailed knowledge of the investigated system. Here, we show the application of computational methods for optimization with multi-level reactor design (MLRD) methodology based on the underlying physical and chemical processes. We optimize a stereoselective reduction of a diketone catalyzed by ketoreductase (Gre2) and Nicotinamidadenindinukleotidphosphat (NADPH) cofactor regeneration with glucose dehydrogenase (GDH). Both enzymes are separately immobilized on magnetic beads forming a packed bed within the microreactor. We derive optimal reactor feed concentrations and enzyme ratios for enhanced performance and a basic economic model in order to maximize the techno-economic performance (TEP) for the first reduction of 5-nitrononane-2,8-dione.


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