Identification and Use of Fluorescent Dyes for Plant Cell Wall Imaging Using High-Throughput Screening

2013 ◽  
pp. 103-109 ◽  
Author(s):  
Charles T. Anderson ◽  
Andrew Carroll
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
High Throughput ◽  
High Throughput Screening ◽  
Plant Cell Wall ◽  
Fluorescent Dyes ◽  
Wall Imaging

High-Throughput Screening of Plant Cell-Wall Composition Using Pyrolysis Molecular Beam Mass Spectroscopy

2009 ◽  
pp. 169-183 ◽  
Author(s):  
Robert Sykes ◽  
Matthew Yung ◽  
Evandro Novaes ◽  
Matias Kirst ◽  
Gary Peter ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Mass Spectroscopy ◽  
High Throughput ◽  
High Throughput Screening ◽  
Molecular Beam ◽  
Plant Cell Wall ◽  
Cell Wall Composition

scholarly journals High-throughput screening of monoclonal antibodies against plant cell wall glycans by hierarchical clustering of their carbohydrate microarray binding profiles

2007 ◽  
Vol 25 (1) ◽  
pp. 37-48 ◽  
Author(s):  
Isabel Moller ◽  
Susan E. Marcus ◽  
Ash Haeger ◽  
Yves Verhertbruggen ◽  
Rene Verhoef ◽  
...  
Keyword(s):  
Cell Wall ◽  
Monoclonal Antibodies ◽  
Hierarchical Clustering ◽  
Plant Cell ◽  
High Throughput ◽  
High Throughput Screening ◽  
Plant Cell Wall

scholarly journals A Label-free, Fast and High-specificity Technique for Plant Cell Wall Imaging and Composition Analysis

2020 ◽  
Author(s):  
Huimin Xu ◽  
Yuanyuan Zhao ◽  
Yuanzhen Suo ◽  
Yayu Guo ◽  
Yi Man ◽  
...  
Keyword(s):  
Cell Wall ◽  
Chemical Composition ◽  
Raman Scattering ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Wall ◽  
Composition Analysis ◽  
Plant Cell Walls ◽  
Label Free ◽  
Wall Imaging

Abstract Background: Cell wall imaging can considerably permit direct visualization of the molecular architecture of cell walls and provide the detailed chemical information on wall polymers, which is imperative to better exploit and use the biomass polymers; however, detailed imaging and quantifying of the native composition and architecture in the cell wall remains challenging.Results: Here, we describe a label-free imaging technology, coherent Raman scattering microscopy (CRS), including coherent anti-Stokes Raman scattering (CARS) microscopy and stimulated Raman scattering (SRS) microscopy, which images the major structures and chemical composition of plant cell walls. The major steps of the procedure are demonstrated, including sample preparation, setting the mapping parameters, analysis of spectral data, and image generation. Applying this rapid approach, which will help researchers understand the highly heterogeneous structures and organization of plant cell walls.Conclusions: This method can potentially be incorporated into label-free microanalyses of plant cell wall chemical composition based on the in situ vibrations of molecules.

Carbohydrate Microarray Technology Applied to High-Throughput Mapping of Plant Cell Wall Glycans Using Comprehensive Microarray Polymer Profiling (CoMPP)

2016 ◽  
pp. 147-165 ◽  
Author(s):  
Stjepan Krešimir Kračun ◽  
Jonatan Ulrik Fangel ◽  
Maja Gro Rydahl ◽  
Henriette Lodberg Pedersen ◽  
Silvia Vidal-Melgosa ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
High Throughput ◽  
Plant Cell Wall ◽  
Microarray Technology

Benzodifuran-based fluorescent brighteners: A novel platform for plant cell wall imaging

2021 ◽  
pp. 110071
Author(s):  
Rosita Diana ◽  
Ugo Caruso ◽  
Francesco Silvio Gentile ◽  
Luigi Di Costanzo ◽  
David Turrà ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Wall Imaging

scholarly journals A label-free, fast and high-specificity technique for plant cell wall imaging and composition analysis

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Huimin Xu ◽  
Yuanyuan Zhao ◽  
Yuanzhen Suo ◽  
Yayu Guo ◽  
Yi Man ◽  
...  
Keyword(s):  
Cell Wall ◽  
Chemical Composition ◽  
Raman Scattering ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Wall ◽  
Composition Analysis ◽  
Plant Cell Walls ◽  
Label Free ◽  
Wall Imaging

Abstract Background New cell wall imaging tools permit direct visualization of the molecular architecture of cell walls and provide detailed chemical information on wall polymers, which will aid efforts to use these polymers in multiple applications; however, detailed imaging and quantification of the native composition and architecture in the cell wall remains challenging. Results Here, we describe a label-free imaging technology, coherent Raman scattering (CRS) microscopy, including coherent anti-Stokes Raman scattering (CARS) microscopy and stimulated Raman scattering (SRS) microscopy, which can be used to visualize the major structures and chemical composition of plant cell walls. We outline the major steps of the procedure, including sample preparation, setting the mapping parameters, analysis of spectral data, and image generation. Applying this rapid approach will help researchers understand the highly heterogeneous structures and organization of plant cell walls. Conclusions This method can potentially be incorporated into label-free microanalyses of plant cell wall chemical composition based on the in situ vibrations of molecules.

scholarly journals Plant cell wall imaging by metabolic click-mediated labelling of rhamnogalacturonan II using azido 3-deoxy-d-manno-oct-2-ulosonic acid

2016 ◽  
Vol 85 (3) ◽  
pp. 437-447 ◽  
Author(s):  
Marie Dumont ◽  
Arnaud Lehner ◽  
Boris Vauzeilles ◽  
Julien Malassis ◽  
Alan Marchant ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Rhamnogalacturonan Ii ◽  
Ulosonic Acid ◽  
Wall Imaging
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