scholarly journals Plant cell wall imaging by metabolic click-mediated labelling of rhamnogalacturonan II using azido 3-deoxy-d-manno-oct-2-ulosonic acid

2016 ◽  
Vol 85 (3) ◽  
pp. 437-447 ◽  
Author(s):  
Marie Dumont ◽  
Arnaud Lehner ◽  
Boris Vauzeilles ◽  
Julien Malassis ◽  
Alan Marchant ◽  
...  
2020 ◽  
Author(s):  
Huimin Xu ◽  
Yuanyuan Zhao ◽  
Yuanzhen Suo ◽  
Yayu Guo ◽  
Yi Man ◽  
...  

Abstract Background: Cell wall imaging can considerably permit direct visualization of the molecular architecture of cell walls and provide the detailed chemical information on wall polymers, which is imperative to better exploit and use the biomass polymers; however, detailed imaging and quantifying of the native composition and architecture in the cell wall remains challenging.Results: Here, we describe a label-free imaging technology, coherent Raman scattering microscopy (CRS), including coherent anti-Stokes Raman scattering (CARS) microscopy and stimulated Raman scattering (SRS) microscopy, which images the major structures and chemical composition of plant cell walls. The major steps of the procedure are demonstrated, including sample preparation, setting the mapping parameters, analysis of spectral data, and image generation. Applying this rapid approach, which will help researchers understand the highly heterogeneous structures and organization of plant cell walls.Conclusions: This method can potentially be incorporated into label-free microanalyses of plant cell wall chemical composition based on the in situ vibrations of molecules.


2021 ◽  
pp. 110071
Author(s):  
Rosita Diana ◽  
Ugo Caruso ◽  
Francesco Silvio Gentile ◽  
Luigi Di Costanzo ◽  
David Turrà ◽  
...  

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Huimin Xu ◽  
Yuanyuan Zhao ◽  
Yuanzhen Suo ◽  
Yayu Guo ◽  
Yi Man ◽  
...  

Abstract Background New cell wall imaging tools permit direct visualization of the molecular architecture of cell walls and provide detailed chemical information on wall polymers, which will aid efforts to use these polymers in multiple applications; however, detailed imaging and quantification of the native composition and architecture in the cell wall remains challenging. Results Here, we describe a label-free imaging technology, coherent Raman scattering (CRS) microscopy, including coherent anti-Stokes Raman scattering (CARS) microscopy and stimulated Raman scattering (SRS) microscopy, which can be used to visualize the major structures and chemical composition of plant cell walls. We outline the major steps of the procedure, including sample preparation, setting the mapping parameters, analysis of spectral data, and image generation. Applying this rapid approach will help researchers understand the highly heterogeneous structures and organization of plant cell walls. Conclusions This method can potentially be incorporated into label-free microanalyses of plant cell wall chemical composition based on the in situ vibrations of molecules.


2011 ◽  
Vol 9 (19) ◽  
pp. 6670 ◽  
Author(s):  
Sergey A. Nepogodiev ◽  
Margherita Fais ◽  
David L. Hughes ◽  
Robert A. Field

1988 ◽  
Vol 182 (2) ◽  
pp. 207-226 ◽  
Author(s):  
Thomas T. Stevenson ◽  
Alan G. Darvill ◽  
Peter Albersheim

1999 ◽  
Vol 274 (19) ◽  
pp. 13098-13104 ◽  
Author(s):  
Tadashi Ishii ◽  
Toshiro Matsunaga ◽  
Patrice Pellerin ◽  
Malcolm A. O’Neill ◽  
Alan Darvill ◽  
...  

1988 ◽  
Vol 179 ◽  
pp. 269-288 ◽  
Author(s):  
Thomas T. Stevenson ◽  
Alan G. Darvill ◽  
Peter Albersheim

Author(s):  
M.A. Zhivetiev ◽  
◽  
A.V. Papkina ◽  
I.A. Graskova ◽  
V.K. Voinikov ◽  
...  

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