Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes Without Mitogen Stimulation for Chromosome Aberration Analysis Using Specific Whole Chromosome DNA Hybridization Probes

2014 ◽  
pp. 171-181 ◽  
Author(s):  
Rupak Pathak ◽  
Pataje G. S. Prasanna
Keyword(s):  
Chromosome Aberration ◽  
Peripheral Blood ◽  
Dna Hybridization ◽  
Peripheral Blood Lymphocytes ◽  
Chromosome Condensation ◽  
Premature Chromosome Condensation ◽  
Mitogen Stimulation ◽  
Blood Lymphocytes ◽  
Hybridization Probes

scholarly journals Chromosome Aberrations as a Biomarker for Genomic Instability in Cell Cultures Originated From Bovines, Canines and Equines Infected With Papillomavirus

2016 ◽  
Vol 4 (1) ◽  
pp. 104-112
Author(s):  
Rachel Siqueira de Queiroz Simões ◽  
Ortrud Monika Barth
Keyword(s):  
Genomic Instability ◽  
Cell Cultures ◽  
Peripheral Blood ◽  
Chromosomal Abnormalities ◽  
Cytogenetic Study ◽  
Peripheral Blood Lymphocytes ◽  
Chromosome Condensation ◽  
Premature Chromosome Condensation ◽  
Blood Lymphocytes ◽  
First Time

Chromosomal abnormalities or aberrations were investigated in ten bovines, three canines and three equines presenting cutaneous papillomatosis. The aim of the present study was to evaluate possible chromosomal abnormalities as a biomarker of genomic instability induced by the interaction of the viral genome with the chromatin of the host cell in peripheral blood lymphocytes and skin warts lesion cultures. Genomic DNA was extracted from blood samples and epithelial biopsies. Amplification products from PCR using degenerated primer pair FAP59/FAP64 were sequenced. Peripheral blood lymphocytes and tumor cell cultures were performed. Structural chromosomal aberrations were verified as associations, chromosomal fragments, centric fusions, open chromatids, gaps, rearrangements and rings. Several levels of premature chromosome condensation were observed in both cultures. Numeric abnormalities as aneuploidy were also detected and hipoployd cells were found in a larger number than the hiperployd cells. This cytogenetic study in detecting premature chromosome condensation in the three species investigated describes by the first time the structural and numerical abnormalities in canines and equines presenting papillomatosis lesions. These abnormalities are similar of the three groups of animals, but virus specific.Int J Appl Sci Biotechnol, Vol 4(1): 104-112

An Infertile Man with Complementary Isochromosome of 46, XY, I(5)(P10),I(5)(Q10): A Case Report

2020 ◽  
Author(s):  
Yu HE ◽  
Bo LIU ◽  
Hui-Ling WU ◽  
Shan HUANG ◽  
Yun-Wei QI ◽  
...  
Keyword(s):  
Case Report ◽  
Chromosome Aberration ◽  
Chromosomal Abnormality ◽  
Peripheral Blood ◽  
Semen Analysis ◽  
Peripheral Blood Lymphocytes ◽  
First Report ◽  
Blood Lymphocytes

Chromosome aberration is associated with infertility and isochromosome is a rare chromosomal abnormality potentially associated with infertility or multiple miscarriages. Here we report a man of 29-yr-old diagnosed of infertility from China with rare isochromosome. Peripheral blood lymphocytes were obtained for chromosome G banding karyotyping. Hormones and other tests were performed. The semen analysis revealed Azoospermia. Complementary isochromosome of 46, XY, i(5)(p10),i(5)(q10) was identified. This is the first report of Complementary isochromosome of 46, XY, i(5)(p10),i(5)(q10).

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