Isolation of Human Neutrophils from Venous Blood

2014 ◽  
pp. 13-18 ◽  
Author(s):  
William M. Nauseef
Keyword(s):  
Venous Blood ◽  
Human Neutrophils

Isolation of Human Neutrophils from Venous Blood

2019 ◽  
pp. 33-42 ◽  
Author(s):  
Silvie Kremserova ◽  
William M. Nauseef
Keyword(s):  
Venous Blood ◽  
Human Neutrophils

The Production of Platelet-Activating Factor during Hemodialysis

1989 ◽  
Vol 12 (12) ◽  
pp. 766-772 ◽  
Author(s):  
C. Tetta ◽  
G. Segoloni ◽  
A. Pacitti ◽  
G. Regis ◽  
M. Salomone ◽  
...  
Keyword(s):  
Dynamic Model ◽  
Neutrophil Elastase ◽  
Plasma Levels ◽  
Proteinase Inhibitor ◽  
Venous Blood ◽  
Platelet Activating Factor ◽  
Human Neutrophils ◽  
Inhibitor Complex ◽  
Neutrophil Aggregation

Regenerated cellulosic membranes (CU) induced the aggregation of plasma-free human neutrophils when recirculated in a dynamic model of dialysis without the patient on the circuit. Neutrophil aggregation was linked to the production of PAF by these cells. In the absence of detectable PAF production, no neutrophil aggregation occurred, as observed during recirculation with polymethylmethacrylate (PMMA) membranes. With polycarbonate (PC), PAF production and aggregation of neutrophils were both almost half the values with CU. PAF production was studied in ten hemodialysis (HD) patients tested twice with CU and once with PC and PMMA membranes. PAF was extracted in the venous blood during filling of the dialyser for 9/20 of patients with CU (3.1 ± 2.9 ng/ml, mean ± 1 S.D.) a membrane that induced marked leukopenia (> 50% of basal values at 15 min), C3a des Arg generation (> 500% at 5 min), and plasma levels of the elastase-alpha1-proteinase inhibitor complex (> 500% at the end of HD). Membranes such as PC and PMMA showing intermediate or low potential to induce leukopenia and C3a des Arg generation, respectively, did not trigger the production and release of PAF in detectable amounts at any interval. However, with PMMA, plasma neutrophil elastase was significantly higher than baseline at the end of dialysis. These levels were not significantly different (p < 0.05) from those observed with CU and PC membranes

Neutrophil Myeloperoxidase Destruction by Ultraviolet Irradiation

1988 ◽  
Vol 46 ◽  
pp. 138-139
Author(s):  
J. Hanker ◽  
B. Giammara ◽  
G. Strauss
Keyword(s):  
Uv Radiation ◽  
Myeloid Leukemia ◽  
Stratospheric Ozone ◽  
Psoriasis Patient ◽  
Whole Body ◽  
Human Neutrophils ◽  
Cupric Nitrate ◽  
The Earth ◽  
Routine Handling ◽  
Uvb Phototherapy

Only a fraction of the UV radiation emitted by the sun reaches the earth; most of the UVB (290-320nm) is eliminated by stratospheric ozone. There is increasing concern, however, that man-made chemicals are damaging this ozone layer. Although the effects of UV on DNA or as a carcinogen are widely known, preleukemia and acute myeloid leukemia (AML) have only rarely been reported in psoriasis patients treated with 8-methoxypsoralen and UV (PUVA). It was therefore of interest to study the effects of UV on the myeloperoxidase (MP) activity of human neutrophils. The peroxidase activity of enriched leukocyte preparations on coverslips was shown cytochemically with a diaminobenzidine medium and cupric nitrate intensification.Control samples (Figs. 1,4,5) of human bloods that were not specifically exposed to UV radiation or light except during routine handling were compared with samples which had been exposed in one of several different ways. One preparation (Fig. 2) was from a psoriasis patient who had received whole-body UVB phototherapy repeatedly.

Ultrastructure of rat alveolar macrophages activated in situ by poly:IC

1987 ◽  
Vol 45 ◽  
pp. 768-769
Author(s):  
A. M. Klinkner ◽  
R. A. Weiss ◽  
A. Kelley ◽  
P. J. Bugelski
Keyword(s):  
Venous Blood ◽  
Intratracheal Instillation ◽  
Buffy Coat ◽  
Fischer 344 Rats ◽  
Blood Monocytes ◽  
Fischer 344 ◽  
Polyinosinic:Polycytidylic Acid ◽  
Macrophage Activator ◽  
Endogenous Peroxidase

Polyinosinic:polycytidylic acid is an inducer of interferon and a macrophage activator. We have found that intratracheal instillation of polyI:C (IT-pI:C) activates rat bronchoalveolar lavage cells (BAL) for a variety of functions. Examination of Giemsa stained, cytocentrifuge preparations showed that IT-pI:C induced a population of BAL not seen in resident BAL. The morphology of these cells suggested that they might be derived from blood monocytes. To test this hypothesis we have examined several populations of macrophages that had been stained for endogenous peroxidase activity as a marker of cells derived from the monocyte-macrophage lineage.Macrophages were obtained from Fischer 344 rats. Peritoneal exudate cells (PEC) were collected by lavage 4 days after i.p. injection of 20 ml 3% thioglycolate. Buffy coat monocytes were separated from venous blood from naive rats.

Application of high-resolution field-emission LVSEM, backscatter imaging, immunogold staining to definition of the spatial distributions of two human neutrophil adherence receptors

1993 ◽  
Vol 51 ◽  
pp. 36-37
Author(s):  
Robert D. Nelson ◽  
Sharon R. Hasslen ◽  
Stanley L. Erlandsen
Keyword(s):  
Cell Surface ◽  
Surface Membrane ◽  
Three Dimensional ◽  
Human Neutrophils ◽  
Spatial Distributions ◽  
Immunogold Staining ◽  
Functional Control ◽  
Neutrophil Adherence ◽  
Definition Of ◽  
Mode Of Attachment

Receptors are commonly defined in terms of number per cell, affinity for ligand, chemical structure, mode of attachment to the cell surface, and mechanism of signal transduction. We propose to show that knowledge of spatial distribution of receptors on the cell surface can provide additional clues to their function and components of functional control.L-selectin and Mac-1 denote two receptor populations on the neutrophil surface that mediate neutrophil-endothelial cell adherence interactions and provide for targeting of neutrophil recruitment to sites of inflammation. We have studied the spatial distributions of these receptors using LVSEM and backscatter imaging of isolated human neutrophils stained with mouse anti-receptor (primary) antibody and goat anti-mouse (secondary) antibody conjugated to 12 nm colloidal gold. This combination of techniques provides for three-dimensional analysis of the expression of these receptors on different surface membrane domains of the neutrophil: the ruffles and microvilli that project from the cell surface, and the cell body between these projecting structures.

Inhibition of Apoptosis in Human Neutrophils by Helicobacter pylori Water-Soluble Surface Proteins

2001 ◽  
Vol 36 (6) ◽  
pp. 589-600 ◽  
Author(s):  
J. S. Kim ◽  
J. M. Kim ◽  
H. C. Jung ◽  
I. S. Song ◽  
C. Y. Kim
Keyword(s):  
Helicobacter Pylori ◽  
Water Soluble ◽  
Surface Proteins ◽  
Human Neutrophils

Author response for "T cell‐derived cytokines enhance the antigen‐presenting capacity of human neutrophils"

2019 ◽  
Author(s):  
Nazanin Samadi ◽  
Dominika Polak ◽  
Claudia Kitzmüller ◽  
Peter Steinberger ◽  
Gerhard J. Zlabinger ◽  
...  
Keyword(s):  
T Cell ◽  
Author Response ◽  
Human Neutrophils ◽  
Antigen Presenting
Sign in / Sign up

Export Citation Format

Share Document