Corneal Regeneration: Use of Extracorneal Stem Cells

2019 ◽  
pp. 123-144 ◽  
Author(s):  
Christian Claude Lachaud ◽  
Abdelkrim Hmadcha ◽  
Bernat Soria
Keyword(s):  
Stem Cells ◽  
Corneal Regeneration

Corneal regeneration by conditioned medium of human uterine cervical stem cells is mediated by TIMP-1 and TIMP-2

2019 ◽  
Vol 180 ◽  
pp. 110-121 ◽  
Author(s):  
Juan Sendon-Lago ◽  
Samuel Seoane ◽  
Anxo Martinez-Ordoñez ◽  
Noemi Eiro ◽  
Jorge Saa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Conditioned Medium ◽  
Corneal Regeneration

Mesenchymal stem cells and corneal regeneration

2013 ◽  
Vol 91 ◽  
pp. 0-0
Author(s):  
A HOPKINSON ◽  
M BRANCH
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Corneal Regeneration

scholarly journals COMPARATIVE STUDY OF THE EFFICACY OF STEM CELLS IN CORNEAL REGENERATION IN A CHEMICAL BURN IN RABBITS

2020 ◽  
Author(s):  
Abdelkrim Salama ◽  
Abdul-Malik Abudunia ◽  
Ali Gamal Al-kaf ◽  
Nabeel Muhsin Ziad ◽  
Azeddine Ibrahimi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Stem Cell Transplantation ◽  
Peer Review ◽  
Cell Transplantation ◽  
Cell Transplant ◽  
Chemical Burn ◽  
Limbal Stem Cells ◽  
Corneal Regeneration ◽  
E Mail

Objectives: This study compares the efficacy of stem cell transplantation in corneal regeneration and restoration of the limbic deficit in an experimental chemical burn in rabbits. Methods: Biopsy was performed of the limbus and the chemical burns for all rabbits, and we collected the amniotic membranes from a pregnant female rabbit. We kept a control group without transplantation, to study spontaneous and natural healing, and we transplanted the stem cells produced in vitro under the corneal epithelium burned. To compare the result, we tested a group for amniotic stem cell transplantation, a group for limbal stem cell graft, and another group for combined transplantation of both types of stem cells. Results: Transplanted rabbits develop permanent unilateral blindness due to a severe limbic deficit. The group receiving only amniotic stem cells shows temporary anatomical improvement without functional recovery. The two groups receiving limbal stem cells alone or combined with amniotic stem cells showed anatomical and functional satisfaction with quick recovery time for the combined transplantation. Conclusions: A simple chemical burn can establish permanent blindness. When the limbic deficit is important, spontaneous healing is not available. Transplantation of stem cell transplant is the only way to repair this deficit and regenerate the cornea. Only limbic stem cells can be sufficient. Amniotic stem cells can support and speed up the healing time when it combined to limbal stem cells graft.  Peer Review History: Received 23 July 2020; Revised 14 August; Accepted 28 August, Available online 15 September 2020 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file Average Peer review marks at initial stage: 5.0/10 Average Peer review marks at publication stage: 7.5/10 Reviewer(s) detail: Name: Dr. Mohamed Amin El-Emam Affiliation: Department of Pharmacology and Therapeutics, Faculty of Pharmacy and Drug Manufacturing, Pharos University in Alexandria (PUA), Alexandria, Egypt. E-mail: [email protected]   Name: Francesco Ferrara Affiliation: USL Umbria 1, Perugia, Italy E-mail: [email protected]   Name: Maged Almezgagi Affiliation: Department of Immunology, Medical College of Qinghai University, Qinghai Xining 810001, China E-mail: [email protected]   Name: Dr. Asia Selman Abdullah Affiliation: University of Basrah, Iraq E-mail: [email protected]   Comments of reviewer(s):

Epithelial stem cells in corneal regeneration and epidermal gene therapy

2009 ◽  
Vol 217 (2) ◽  
pp. 217-228 ◽  
Author(s):  
G Pellegrini ◽  
P Rama ◽  
F Mavilio ◽  
M De Luca
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Epithelial Stem Cells ◽  
Corneal Regeneration

scholarly journals Regenerative Approaches and Future Trends for the Treatment of Corneal Burn Injuries

2021 ◽  
Vol 10 (2) ◽  
pp. 317
Author(s):  
Gabriele Saccu ◽  
Valeria Menchise ◽  
Cristina Giordano ◽  
Daniela Delli Castelli ◽  
Walter Dastrù ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ocular Surface ◽  
Burn Injury ◽  
Treatment Strategies ◽  
Burn Injuries ◽  
Ocular Tissue ◽  
Epithelial Stem Cells ◽  
Surface Anatomy ◽  
Corneal Regeneration ◽  
And Function

Ocular chemical and thermal burns are frequent causes of hospitalization and require immediate interventions and care. Various surgical and pharmacological treatment strategies are employed according to damage severity. Controlling inflammation and neovascularization while promoting normal ocular surface anatomy and function restoration is the principal aim. In the most severe cases, when epithelial healing is severely affected, reconstruction of the ocular surface may be a valid option, which, however, requires expertise, adequate instruments, and qualified donors. Numerous endogenous and exogenous strategies have been considered for corneal repair. Among these, stem cells and their derivatives have offered numerous attractive possibilities in finding an effective way in stimulating corneal regeneration. Limbal epithelial stem cells and mesenchymal cells from the ocular tissue as well as from various sources have demonstrated their effectiveness in dampening neovascularization, scarring, and inflammation, while promoting epithelialization of the injured cornea. Moreover, a plethora of cytokines and growth factors, and extracellular vesicles, which constitute the secretome of these cells, work in concert to enhance wound healing. In this review, we provide an update on the recent potential therapeutic avenues and clinical applications of stem cells and their products in corneal regeneration after burn injury, as well as current imaging strategies for monitoring therapeutic efficacy and damage resolution.

scholarly journals Bi-compartmentalized stem cell organization of the corneal limbal niche

2020 ◽  
Author(s):  
Olivia Farrelly ◽  
Yoko Suzuki-Horiuchi ◽  
Megan Brewster ◽  
Paola Kuri ◽  
Sixia Huang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Stem Cell Niche ◽  
Tissue Architecture ◽  
Corneal Regeneration ◽  
Cell Organization ◽  
Cell Niche ◽  
Slow Cycling ◽  
And Function

AbstractStem cells exist in precise locations within tissues, yet how their organization supports tissue architecture and function is poorly understood. The limbus is the presumptive stem cell niche of the corneal epithelium. Here, we visualize the live limbus and track the activity of single stem cells in their native environment by 2-photon microscopy. We identify previously unknown niche compartments and show that long implicated slow-cycling cells form separate lineages in the outer limbus, with only local clonal dynamics. Instead, we find distinct stem cells in the pericorneal limbus to be required for corneal regeneration. Unbiased photolabeling captures their progeny exiting the niche, then moving centripetally in unison before undergoing terminal differentiation. This study demonstrates how a compartmentalized stem cell organization coordinates tissue regeneration.One Sentence SummaryIn vivo live imaging of the regenerating cornea reveals distinct stem cell activities in the limbal niche

Limbal Stem Cells and Corneal Regeneration

2012 ◽  
pp. 223-240
Author(s):  
Geeta K. Vemuganti ◽  
Murali Mohan Sagar Baila ◽  
Shubha Tiwari
Keyword(s):  
Stem Cells ◽  
Limbal Stem Cells ◽  
Corneal Regeneration

scholarly journals Human epithelial stem cells in corneal regeneration and epidermal gene therapy

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Michele De Luca ◽  
Paolo Rama ◽  
Fulvio Mavilio ◽  
Graziella Pellegrini
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Epithelial Stem Cells ◽  
Corneal Regeneration
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