Epithelial stem cells in corneal regeneration and epidermal gene therapy

2009 ◽  
Vol 217 (2) ◽  
pp. 217-228 ◽  
Author(s):  
G Pellegrini ◽  
P Rama ◽  
F Mavilio ◽  
M De Luca
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Epithelial Stem Cells ◽  
Corneal Regeneration

scholarly journals Human epithelial stem cells in corneal regeneration and epidermal gene therapy

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Michele De Luca ◽  
Paolo Rama ◽  
Fulvio Mavilio ◽  
Graziella Pellegrini
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Epithelial Stem Cells ◽  
Corneal Regeneration

scholarly journals Regenerative Approaches and Future Trends for the Treatment of Corneal Burn Injuries

2021 ◽  
Vol 10 (2) ◽  
pp. 317
Author(s):  
Gabriele Saccu ◽  
Valeria Menchise ◽  
Cristina Giordano ◽  
Daniela Delli Castelli ◽  
Walter Dastrù ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ocular Surface ◽  
Burn Injury ◽  
Treatment Strategies ◽  
Burn Injuries ◽  
Ocular Tissue ◽  
Epithelial Stem Cells ◽  
Surface Anatomy ◽  
Corneal Regeneration ◽  
And Function

Ocular chemical and thermal burns are frequent causes of hospitalization and require immediate interventions and care. Various surgical and pharmacological treatment strategies are employed according to damage severity. Controlling inflammation and neovascularization while promoting normal ocular surface anatomy and function restoration is the principal aim. In the most severe cases, when epithelial healing is severely affected, reconstruction of the ocular surface may be a valid option, which, however, requires expertise, adequate instruments, and qualified donors. Numerous endogenous and exogenous strategies have been considered for corneal repair. Among these, stem cells and their derivatives have offered numerous attractive possibilities in finding an effective way in stimulating corneal regeneration. Limbal epithelial stem cells and mesenchymal cells from the ocular tissue as well as from various sources have demonstrated their effectiveness in dampening neovascularization, scarring, and inflammation, while promoting epithelialization of the injured cornea. Moreover, a plethora of cytokines and growth factors, and extracellular vesicles, which constitute the secretome of these cells, work in concert to enhance wound healing. In this review, we provide an update on the recent potential therapeutic avenues and clinical applications of stem cells and their products in corneal regeneration after burn injury, as well as current imaging strategies for monitoring therapeutic efficacy and damage resolution.

scholarly journals Corneal Stem Cells as a Source of Regenerative Cell-Based Therapy

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Jasmin S. Nurković ◽  
Radiša Vojinović ◽  
Zana Dolićanin
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Therapeutic Potential ◽  
Corneal Stroma ◽  
Epithelial Stem Cells ◽  
Corneal Surface ◽  
Cell Based Therapy ◽  
Corneal Regeneration ◽  
Successful Transplantation ◽  
Stromal Stem Cells

In the past few years, intensive research has focused on corneal stem cells as an unlimited source for cell-based therapy in regenerative ophthalmology. Today, it is known that the cornea has at least two types of stem cells: limbal epithelial stem cells (LESCs) and corneal stromal stem cells (CSSCs). LESCs are used for regeneration of corneal surface, while CSSCs are used for regeneration of corneal stroma. Until now, various approaches and methods for isolation of LESCs and CSSCs and their successful transplantation have been described and tested in several preclinical studies and clinical trials. This review describes in detail phenotypic characteristics of LESCs and CSSCs and discusses their therapeutic potential in corneal regeneration. Since efficient and safe corneal stem cell-based therapy is still a challenging issue that requires continuous cooperation between researchers, clinicians, and patients, this review addresses the important limitations and suggests possible strategies for improvement of corneal stem cell-based therapy.

1417: Mesenchymal Stem Cells Alone or Modified with ENOS Restore Erectile Function in the Aged Rat: Cell-Based Gene Therapy for Erectile Dysfunction

2004 ◽  
Vol 171 (4S) ◽  
pp. 373-373
Author(s):  
Trinity J. Bivalacqua ◽  
Mustafa F. Usta ◽  
Hunter C. Champion ◽  
Weiwen Deng ◽  
Philip J. Kadowitz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Mesenchymal Stem Cells ◽  
Erectile Dysfunction ◽  
Erectile Function ◽  
Aged Rat

A common mechanism links activities of butyrate in the colon

2017 ◽  
Author(s):  
Mohit S. Verma ◽  
Michael J. Fink ◽  
Gabriel L Salmon ◽  
Nadine Fornelos ◽  
Takahiro E. Ohara ◽  
...  
Keyword(s):  
Stem Cells ◽  
Histone Deacetylases ◽  
Biological Activities ◽  
Short Chain Fatty Acids ◽  
Common Mechanism ◽  
Epithelial Stem Cells ◽  
Degree Of Unsaturation ◽  
Structure Activity ◽  
Starting Point ◽  
New Compounds

Two biological activities of butyrate in the colon (suppression of proliferation of colonic epithelial stem cells and inflammation) correlate with inhibition of histone deacetylases. Cellular and biochemical studies of molecules similar in structure to butyrate, but different in molecular details (functional groups, chain-length, deuteration, oxidation level, fluorination, or degree of unsaturation) demonstrated that these activities were sensitive to molecular structure, and were compatible with the hypothesis that butyrate acts by binding to the Zn<sup>2+</sup> in the catalytic site of histone deacetylases. Structure-activity relationships drawn from a set of 36 compounds offer a starting point for the design of new compounds targeting the inhibition of histone deacetylases. The observation that butyrate was more potent than other short-chain fatty acids is compatible with the hypothesis that crypts evolved (at least in part), to separate stem cells at the base of crypts from butyrate produced by commensal bacteria.

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