8 Coordination of Fungal Secondary Metabolism and Development

2020 ◽  
pp. 173-205 ◽  
Author(s):  
Jennifer Gerke ◽  
Anna M. Köhler ◽  
Cindy Meister ◽  
Karl G. Thieme ◽  
Hugo Amoedo ◽  
...  
Keyword(s):  
Secondary Metabolism ◽  
Fungal Secondary Metabolism

Screening of tenuazonic acid production-inducing compounds and identification of NPD938 as a regulator of fungal secondary metabolism

2021 ◽  
Author(s):  
Takayuki Motoyama ◽  
Tomoaki Ishii ◽  
Takashi Kamakura ◽  
Hiroyuki Osada
Keyword(s):  
Secondary Metabolism ◽  
Gene Clusters ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene ◽  
Tenuazonic Acid ◽  
Cellular Functions ◽  
Biosynthetic Gene Clusters ◽  
Chemical Library ◽  
Tea Production ◽  
Fungal Secondary Metabolism

Abstract The control of secondary metabolism in fungi is essential for the regulation of various cellular functions. In this study, we searched the RIKEN Natural Products Depository (NPDepo) chemical library for inducers of tenuazonic acid (TeA) production in the rice blast fungus Pyricularia oryzae and identified NPD938. NPD938 transcriptionally induced TeA production. We explored the mode of action of NPD938 and observed that this compound enhanced TeA production via LAE1, a global regulator of fungal secondary metabolism. NPD938 could also induce production of terpendoles and pyridoxatins in Tolypocladium album RK99-F33. Terpendole production was induced transcriptionally. We identified the pyridoxatin biosynthetic gene cluster among transcriptionally induced secondary metabolite biosynthetic gene clusters. Therefore, NPD938 is useful for the control of fungal secondary metabolism.

A float culture method for fungal secondary metabolism study using hydrophilic polyvinylidene fluoride membranes

2020 ◽  
Vol 599 ◽  
pp. 113722
Author(s):  
Fei Tian ◽  
Sang Yoo Lee ◽  
So Young Woo ◽  
Hwa Young Choi ◽  
Hyang Sook Chun
Keyword(s):  
Secondary Metabolism ◽  
Polyvinylidene Fluoride ◽  
Culture Method ◽  
Fungal Secondary Metabolism ◽  
Metabolism Study

scholarly journals Interspecies Genomic Variation and Transcriptional Activeness of Secondary Metabolism-Related Genes in Aspergillus Section Fumigati

2021 ◽  
Vol 2 ◽  
Author(s):  
Hiroki Takahashi ◽  
Maiko Umemura ◽  
Akihiro Ninomiya ◽  
Yoko Kusuya ◽  
Masaaki Shimizu ◽  
...  
Keyword(s):  
Secondary Metabolism ◽  
Gene Clusters ◽  
Culture Conditions ◽  
Genomic Variation ◽  
Comparative Genomic ◽  
Closely Related Species ◽  
Unknown Reason ◽  
Neosartorya Fischeri ◽  
Fungal Secondary Metabolism ◽  
Respective Species

Filamentous fungi produce various bioactive compounds that are biosynthesized by sets of proteins encoded in biosynthesis gene clusters (BGCs). For an unknown reason, many BGCs are transcriptionally silent in laboratory conditions, which has hampered the discovery of novel fungal compounds. The transcriptional reactiveness of fungal secondary metabolism is not fully understood. To gain the comprehensive view, we conducted comparative genomic and transcriptomic analyses of nine closely-related species of Aspergillus section Fumigati (A. fumigatus, A. fumigatiaffinis, A. novofumigatus, A. thermomutatus, A. viridinutans, A. pseudoviridinutans, A. lentulus, A. udagawae, and Neosartorya fischeri). For expanding our knowledge, we newly sequenced genomes of A. viridinutans and A. pseudoviridinutans, and reassembled and reannotated the previously released genomes of A. lentulus and A. udagawae. Between 34 and 84 secondary metabolite (SM) backbone genes were identified in the genomes of these nine respective species, with 8.7–51.2% being unique to the species. A total of 247 SM backbone gene types were identified in the nine fungi. Ten BGCs are shared by all nine species. Transcriptomic analysis using A. fumigatus, A. lentulus, A. udagawae, A. viridinutans, and N. fischeri was conducted to compare expression levels of all SM backbone genes in four different culture conditions; 32–83% of SM backbone genes in these species were not expressed in the tested conditions, which reconfirmed that large part of fungal SM genes are hard to be expressed. The species-unique SM genes of the five species were expressed with lower frequency (18.8% in total) than the SM genes that are conserved in all five species (56%). These results suggest that the expression tendency of BGCs is correlated with their interspecies distribution pattern. Our findings increase understanding of the evolutionary processes associated with the regulation of fungal secondary metabolism.

Fungal Secondary Metabolism

2021 ◽  
Author(s):  
Francesco Vinale ◽  
Krishnapillai Sivasithamparam ◽  
Susanne Zeilinger ◽  
Santiago Gutiérrez
Keyword(s):  
Secondary Metabolism ◽  
Fungal Secondary Metabolism

Mycophenolic acid production by Penicillium brevicompactum in two media

1979 ◽  
Vol 25 (8) ◽  
pp. 940-943 ◽  
Author(s):  
D. L. Doerfler ◽  
C. D. Bartman ◽  
I. M. Campbell
Keyword(s):  
Secondary Metabolism ◽  
Mycophenolic Acid ◽  
Acid Production ◽  
Solid Culture ◽  
Simple Medium ◽  
Penicillium Brevicompactum ◽  
Fungal Secondary Metabolism

Penicillium brevicompactum produces mycophenolic acid as it grows vegetatively, not only on a simple medium where growth is slow but also on a richer medium where growth is less restricted. The implications of this finding on the association of fungal secondary metabolism with the idiophase in liquid and solid culture are discussed.

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