Mycophenolic acid production by Penicillium brevicompactum in two media

1979 ◽  
Vol 25 (8) ◽  
pp. 940-943 ◽  
Author(s):  
D. L. Doerfler ◽  
C. D. Bartman ◽  
I. M. Campbell
Keyword(s):  
Secondary Metabolism ◽  
Mycophenolic Acid ◽  
Acid Production ◽  
Solid Culture ◽  
Simple Medium ◽  
Penicillium Brevicompactum ◽  
Fungal Secondary Metabolism

Penicillium brevicompactum produces mycophenolic acid as it grows vegetatively, not only on a simple medium where growth is slow but also on a richer medium where growth is less restricted. The implications of this finding on the association of fungal secondary metabolism with the idiophase in liquid and solid culture are discussed.

Mycophenolic acid is produced during balanced growth of Penicillium brevicompactum

1977 ◽  
Vol 23 (1) ◽  
pp. 20-27 ◽  
Author(s):  
Carter P. Nulton ◽  
Iain M. Campbell
Keyword(s):  
Dilution Rate ◽  
Secondary Metabolite ◽  
Mycophenolic Acid ◽  
Continuous Flow ◽  
Acid Production ◽  
Flow System ◽  
Metabolic System ◽  
Balanced Growth ◽  
Secondary Metabolite Biosynthesis ◽  
Penicillium Brevicompactum

When Penicillium brevicompactum is grown on Czapek Dox medium in the surface or sub merged mode as batch or continuous-flow cultures, mycophenolic acid is produced. Unlike the classical secondary metabolic system, 6-methylsalicylic acid production by P. patulum, mycophenolic acid is formed independently of dilution rate in a flow system. Discounting the possibility that strains of P. brevicompactum that produce mycophenolic acid are mutants defective in the control of secondary metabolite biosynthesis, we conclude that mycophenolic acid production is not regulated as part of a non-vegetative genome. An invertase (EC 3.2.1.26) activity has been encountered in both P. brevicompactum and P. patulum.

Evaluation of mycophenolic acid production by Penicillium brevicompactum MUCL 19011 in batch and continuous submerged cultures

2010 ◽  
Vol 50 (3) ◽  
pp. 99-103 ◽  
Author(s):  
Fatemeh Ardestani ◽  
Seyed Safa-ali Fatemi ◽  
Bagher Yakhchali ◽  
Seyed Morteza Hosseyni ◽  
Ghasem Najafpour
Keyword(s):  
Mycophenolic Acid ◽  
Acid Production ◽  
Submerged Cultures ◽  
Penicillium Brevicompactum

scholarly journals Mycophenolic Acid Production by Penicillium brevicompactum on Solid Media

1981 ◽  
Vol 41 (3) ◽  
pp. 729-736 ◽  
Author(s):  
C. D. Bartman ◽  
D. L. Doerfler ◽  
B. A. Bird ◽  
A. T. Remaley ◽  
J. N. Peace ◽  
...  
Keyword(s):  
Mycophenolic Acid ◽  
Acid Production ◽  
Solid Media ◽  
Penicillium Brevicompactum

scholarly journals Mycophenolic acid production by drug-resistant and methionine or glutamic-acid requiring mutants of Penicillium brevicompactum.

1987 ◽  
Vol 51 (9) ◽  
pp. 2509-2514 ◽  
Author(s):  
Hachiro OZAKI ◽  
Masaru ISHIHARA ◽  
Takao KIDA ◽  
Shigeru YAMANAKA ◽  
Hiroshiro SHIBAI
Keyword(s):  
Glutamic Acid ◽  
Mycophenolic Acid ◽  
Acid Production ◽  
Drug Resistant ◽  
Penicillium Brevicompactum

First report of mycophenolic acid production by Eurotium repens isolated from agricultural and indoor environments

2014 ◽  
Vol 7 (3) ◽  
pp. 321-328 ◽  
Author(s):  
V. Séguin ◽  
S. Gente ◽  
N. Heutte ◽  
P. Vérité ◽  
V. Kientz-Bouchart ◽  
...  
Keyword(s):  
Mycophenolic Acid ◽  
Acid Production ◽  
Molecular Techniques ◽  
Indoor Environments ◽  
Future Studies ◽  
Fungal Identification ◽  
Gas And Liquid Chromatography ◽  
Penicillium Brevicompactum ◽  
Major Producer ◽  
First Time

Mycophenolic acid is considered as an immunosuppressive pharmaceutical drug, but also as a mycotoxin due to its undesirable presence in various feedstuffs. Although Penicillium brevicompactum has been historically identified as the major producer of mycophenolic acid, this study reports for the first time the production of mycophenolic acid by isolates of Eurotium repens collected from agricultural and indoor environments. Fungal identification was achieved by scanning electron microscopy and molecular techniques, and mycophenolic acid production was confirmed by two analytical methods (gas and liquid chromatography coupled to mass spectrometry). These findings suggest that mycophenolic acid production and immunosuppressive properties of other closely related Eurotium should be evaluated in future studies.

scholarly journals Comparative Study for the Production of Mycophenolic Acid using Penicillium brevicompactum in Batch, Fed-batch and Continuous Fermentation Process

2021 ◽  
Vol 12 (1) ◽  
pp. 366-376
Keyword(s):  
Mycophenolic Acid ◽  
Acid Production ◽  
Batch Fermentation ◽  
Fermentation Process ◽  
Continuous Fermentation ◽  
Operating Conditions ◽  
Submerged Cultivation ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Penicillium Brevicompactum

Mycophenolic acid (MPA) is, due to its immunosuppressive and biological activities, a potential compound. It is the secondary metabolite produced in submerged cultivation by the microfungus Penicillium brevicompactum. Batch, fed-batch, and continuous mode of cultivation for mycophenolic acid production were performed and compared in the current work. To increase productivity, mycophenolic acid production was studied in batch, fed-batch, and continuous bioreactors. These experiments were conducted with a 2.5 L working volume in a 3.7 L continuous stirred tank bioreactor. In all cases, operating conditions such as temperature, pH, agitation, and aeration, 28 °C, 5.5, 200 rpm, and 2 vvm, respectively, were the same. In fed-batch fermentation, the MPA concentration obtained was 1.91 g/L higher than the value obtained in batch culture, 1.55 g/L, while in continuous fermentation, 1.67 g/L was obtained. The mycophenolic acid productivity obtained in the continuous fermentation process was 0.025 g/L/h, which was maximum MPA productivity, compared to 0.007 g/L/h in the fed-batch fermentation process and 0.006 g/L/h in the batch fermentation process. The impact of substrate inhibition on the product formation can effectively bring down by continuous fermentation processes. The MPA productivity was increased in continuous fermentation relative to batch and fed-batch processing. The finding indicates that continuous culture of Penicillium brevicompactum is a promising strategy for the synthesis of mycophenolic acid.

Screening of tenuazonic acid production-inducing compounds and identification of NPD938 as a regulator of fungal secondary metabolism

2021 ◽  
Author(s):  
Takayuki Motoyama ◽  
Tomoaki Ishii ◽  
Takashi Kamakura ◽  
Hiroyuki Osada
Keyword(s):  
Secondary Metabolism ◽  
Gene Clusters ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene ◽  
Tenuazonic Acid ◽  
Cellular Functions ◽  
Biosynthetic Gene Clusters ◽  
Chemical Library ◽  
Tea Production ◽  
Fungal Secondary Metabolism

Abstract The control of secondary metabolism in fungi is essential for the regulation of various cellular functions. In this study, we searched the RIKEN Natural Products Depository (NPDepo) chemical library for inducers of tenuazonic acid (TeA) production in the rice blast fungus Pyricularia oryzae and identified NPD938. NPD938 transcriptionally induced TeA production. We explored the mode of action of NPD938 and observed that this compound enhanced TeA production via LAE1, a global regulator of fungal secondary metabolism. NPD938 could also induce production of terpendoles and pyridoxatins in Tolypocladium album RK99-F33. Terpendole production was induced transcriptionally. We identified the pyridoxatin biosynthetic gene cluster among transcriptionally induced secondary metabolite biosynthetic gene clusters. Therefore, NPD938 is useful for the control of fungal secondary metabolism.

Sign in / Sign up

Export Citation Format

Share Document