Single-Cell Metabolomics by Mass Spectrometry Imaging

2021 ◽  
pp. 69-82
Author(s):  
Maria Emilia Dueñas ◽  
Young Jin Lee
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Mass Spectrometry Imaging ◽  
Cell Metabolomics

Single-cell Metabolomics Analysis by Microfluidics and Mass Spectrometry: Recent New Advances

2020 ◽  
Vol 4 (3) ◽  
pp. 198-209 ◽  
Author(s):  
Disheng Feng ◽  
Tianrun Xu ◽  
Hang Li ◽  
Xianzhe Shi ◽  
Guowang Xu
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Metabolomics Analysis ◽  
Cell Metabolomics

scholarly journals Morphometric Cell Classification for Single‐Cell MALDI‐Mass Spectrometry Imaging

2020 ◽  
Vol 59 (40) ◽  
pp. 17447-17450 ◽  
Author(s):  
Klára Ščupáková ◽  
Frédéric Dewez ◽  
Axel K. Walch ◽  
Ron M. A. Heeren ◽  
Benjamin Balluff
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Mass Spectrometry Imaging ◽  
Maldi Mass Spectrometry ◽  
Cell Classification ◽  
Maldi Mass Spectrometry Imaging

scholarly journals Advances in mass spectrometry based single-cell metabolomics

The Analyst ◽  
2019 ◽  
Vol 144 (3) ◽  
pp. 782-793 ◽  
Author(s):  
Kyle D. Duncan ◽  
Jonas Fyrestam ◽  
Ingela Lanekoff
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Biological Activities ◽  
Health And Disease ◽  
Cell Metabolomics

Single cell metabolomics using mass spectrometry can contribute to understanding biological activities in health and disease.

scholarly journals Recent advances in single-cell MALDI mass spectrometry imaging and potential clinical impact

2011 ◽  
Vol 8 (5) ◽  
pp. 591-604 ◽  
Author(s):  
Kristin J Boggio ◽  
Emmanuel Obasuyi ◽  
Ken Sugino ◽  
Sacha B Nelson ◽  
Nathalie YR Agar ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Mass Spectrometry Imaging ◽  
Maldi Mass Spectrometry ◽  
Clinical Impact ◽  
Recent Advances ◽  
Potential Clinical Impact ◽  
Maldi Mass Spectrometry Imaging

Metabolic Imaging at the Single-Cell Scale: Recent Advances in Mass Spectrometry Imaging

2019 ◽  
Vol 12 (1) ◽  
pp. 201-224 ◽  
Author(s):  
Ian S. Gilmore ◽  
Sven Heiles ◽  
Cornelius L. Pieterse
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Mass Spectrometry Imaging ◽  
Secondary Ion Mass Spectrometry ◽  
Cell Types ◽  
Metabolic Imaging ◽  
Spatially Resolved ◽  
Recent Advances ◽  
Challenges And Opportunities ◽  
Future Challenges

There is an increasing appreciation that every cell, even of the same type, is different. This complexity, when additionally combined with the variety of different cell types in tissue, is driving the need for spatially resolved omics at the single-cell scale. Rapid advances are being made in genomics and transcriptomics, but progress in metabolomics lags. This is partly because amplification and tagging strategies are not suited to dynamically created metabolite molecules. Mass spectrometry imaging has excellent potential for metabolic imaging. This review summarizes the recent advances in two of these techniques: matrix-assisted laser desorption ionization (MALDI) and secondary ion mass spectrometry (SIMS) and their convergence in subcellular spatial resolution and molecular information. The barriers that have held back progress such as lack of sensitivity and the breakthroughs that have been made including laser-postionization are highlighted as well as the future challenges and opportunities for metabolic imaging at the single-cell scale.

Sign in / Sign up

Export Citation Format

Share Document